Oligonucleotide microarray with a minimal number of probes for the detection and identification of thirteen genera of plant viruses

Zhang, Yongjiang; Yin, Jun; Li, Guifen; Li, Mingfu; Huang, Xin; Chen, Hongjun; Zhao, Wenran; Zhu, Shuifang

doi:10.1016/j.jviromet.2010.03.010

Cited by 23 publications

(20 citation statements)

References 25 publications

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“…When designed, a DNA microarray can be used routinely as a test for the presence of various phytopathogens (Zhang et al 2010;Tiberini and Barba 2012). However, to our knowledge, none of the described DNA microarrays is suitable for the detection of microbial, maize pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…In total, it gives more than 180 K probes; thereby, it is classified as a high-density microarray and is one of the most complex microarrays for the phytopathogen detection. For example, a DNA chip designed to detect and identify thirteen genera of plant viruses contained only 345 probes targeting 169 phytopathogen species (Zhang et al 2010). For plant viroid detection, only 103 probes were used to detect 37 different species (Zhang et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…For example, a DNA chip designed to detect and identify thirteen genera of plant viruses contained only 345 probes targeting 169 phytopathogen species (Zhang et al 2010). For plant viroid detection, only 103 probes were used to detect 37 different species (Zhang et al 2010). Other authors (Engel et al 2010;Nicolaisen 2011) have also used a small number of probes designed only for a chosen region of genome of the tested microorganism.…”

Section: Discussionmentioning

confidence: 99%

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DNA microarray-based detection and identification of bacterial and viral pathogens of maize

et al. 2017

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We describe the construction and evaluation of DNA microarray for simultaneous detection and identification of five microbial pathogens of maize: Pantoea ananatis, P. agglomerans, Enterobacter cloaceae subsp. dissolvens, Maize dwarf mosaic virus (MDMV) and Sugarcane mosaic virus (SCMV). Unlike other DNA microarrays described, our microarray comprises probes targeting the whole genomes of the tested pathogens. Control probes are complementary to genomes of closely related microorganisms that are nonpathogenic to maize and against maize and human genome sequences in order to avoid the potential false-positive results. Obtained results indicate that the fluorescence signals from pathogen and control probes are well distinguished in all performed experiments. The microarray's performance was compared with classical PCR-based pathogen detection method, and the versatility of the assay was tested in silico.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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DNA microarray-based detection and identification of bacterial and viral pathogens of maize

et al. 2017

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“…This method was used detection for potato viruses such as Potato virus A (PVA), Potato virus M (PVM), Potato virus S (PVS), PVX, PVY and PLRV and cucurbit-infecting plant viruses (Bystricka et al, 2005;Lee et al, 2003). It is also able to identify any plant virus at the genus level and can differentiate relevant strains (Hammond, 2011;Wang et al, 2002;2003;Zhang et al, 2010). Recently, a large-scale oligonucleotide (LSON) chip were developed to detect and to identify 538 plant viruses (Nam et al, 2014).…”

Section: Microarray (Oligonucleotide Array)mentioning

confidence: 99%

A Review of Detection Methods for the Plant Viruses

Jeong¹,

Ju²,

Noh³

2014

Research in Plant Disease

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The early and accurate detection of plant viruses is an essential component to control those. Because the globalization of trade by free trade agreement (FTA) and the rapid climate change promote the country-tocountry transfer of viruses and their hosts and vectors, diagnosis of viral diseases is getting more important. Because symptoms of viral diseases are not distinct with great variety and are confused with those of abiotic stresses, symptomatic diagnosis may not be appropriate. From the last three decades, enzyme-linked immunosorbent assays (ELISAs), developed based on serological principle, have been widely used. However, ELISAs to detect plant viruses decrease due to some limitations such as availability of antibody for target virus, cost to produce antibody, requirement of large volume of sample, and time to complete ELISAs. Many advanced techniques allow overcoming demerits of ELISAs. Since the polymerase chain reaction (PCR) developed as a technique to amplify target DNA, PCR evolved to many variants with greater sensitivity than ELISAs. Many systems of plant virus detection are reviewed here, which includes immunological-based detection system, PCR techniques, and hybridization-based methods such as microarray. Some of techniques have been used in practical, while some are still under developing to get the level of confidence for actual use.

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“…Panax notoginseng virus (Zhang , 2010). 1982 (Lee , 1982), (Watermelon mosaic virus, WMV, WMV2 ) (Jung , 2013).…”

Section: Potyvirusmentioning

confidence: 99%

Characteristics of Watermelon Mosaic Virus Transmission Occurring in Korean Ginseng

Choi¹,

Cho²,

Chung³

et al. 2014

Research in Plant Disease

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Korean ginseng (Panax ginseng) is the most popular herb for medical purpose in Korea. Recently, viral diseases from Korean ginseng showing various degrees of severe mottling, variegation and mosaic symptoms have caused quantity losses of Korean ginseng in a large number of farms. Watermelon mosaic virus (named WMVgin) was identified as a causal agent for the disease of Korean ginseng. Interestingly, WMV-gin failed to infect both Korean ginseng plant and susceptible host species including cucurbitaceous plants by mechanical inoculation. However, WMV-gin could successfully infect Korean ginseng by transmission of two aphid species (Myzus persicae and Aphis gossypii). It is likely that transmission of WMV-gin was done by both the aphid species during feeding behavior of the two aphid species on Korean ginseng, though the aphids dislike feeding in Korea ginseng. Similarly, a strain of WMV (WMV-wm) isolated from watermelon was transmitted successfully to Korean ginseng plant by the two aphid species, but not by mechanical inoculations. Transmission assays using M. persicae and A. gossypii clearly showed both WMV-gin and WMV-wm were not transmitted from infected Korean ginseng plant to cucurbit species that are good host species for WMV. These results suggest WMV disease occurring in Korean ginseng plant can be controlled by ecological approaches.

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Oligonucleotide microarray with a minimal number of probes for the detection and identification of thirteen genera of plant viruses

Cited by 23 publications

References 25 publications

DNA microarray-based detection and identification of bacterial and viral pathogens of maize

DNA microarray-based detection and identification of bacterial and viral pathogens of maize

A Review of Detection Methods for the Plant Viruses

Characteristics of Watermelon Mosaic Virus Transmission Occurring in Korean Ginseng

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