Oligonucleotides‐decorated‐poly(<i>N</i>‐vinyl pyrrolidone) nanogels for gene delivery

Dispenza, Clelia; Adamo, Giorgia; Sabatino, Maria Antonietta; Grimaldi, Natascia; Bulone, Donatella; Bondı̀, Maria Luisa; Rigogliuso, Salvatrice; Ghersi, Giulio

doi:10.1002/app.39774

Cited by 28 publications

(20 citation statements)

References 25 publications

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“…As a result, HA shielding of PEI/pDNA nanogels ensured an effectively internalization of nanogels into human mesenchymal stem cells (hMSCs) and HeLa cells via receptors located in the plasma membrane, recommending these nanocarriers as gene delivery vehicles to induce stem cell differentiation. Dispenza et al (2014) reported the formulation and characterization of biocompatible PVP-AA nanogels surface bioconjugated with a single strand oligonucleotide (ODN). The bioconjugated nanogels exhibited colloidal stability and an excellent ability/capacity to bypass cell membrane, accumulating in perinuclear area of the cytoplasm.…”

Section: Bioconjugated Nanogels As Versatile Nanocarriersmentioning

confidence: 99%

Basic concepts and recent advances in nanogels as carriers for medical applications

Neamţu¹,

Rusu²,

Diaconu³

et al. 2017

Drug Delivery

368

219

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Nanogels in biomedical field are promising and innovative materials as dispersions of hydrogel nanoparticles based on crosslinked polymeric networks that have been called as next generation drug delivery systems due to their relatively high drug encapsulation capacity, uniformity, tunable size, ease of preparation, minimal toxicity, stability in the presence of serum, and stimuli responsiveness. Nanogels show a great potential in chemotherapy, diagnosis, organ targeting and delivery of bioactive substances. The main subjects reviewed in this article concentrates on: (i) Nanogel assimilation in the nanomedicine domain; (ii) Features and advantages of nanogels, the main characteristics, such as: swelling capacity, stimuli sensitivity, the great surface area, functionalization, bioconjugation and encapsulation of bioactive substances, which are taken into account in designing the structures according to the application; some data on the advantages and limitations of the preparation techniques; (iii) Recent progress in nanogels as a carrier of genetic material, protein and vaccine. The majority of the scientific literature presents the multivalency potential of bioconjugated nanogels in various conditions. Today's research focuses over the overcoming of the restrictions imposed by cost, some medical requirements and technological issues, for nanogels' commercial scale production and their integration as a new platform in biomedicine.

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Section: Bioconjugated Nanogels As Versatile Nanocarriersmentioning

confidence: 99%

Basic concepts and recent advances in nanogels as carriers for medical applications

Neamţu¹,

Rusu²,

Diaconu³

et al. 2017

Drug Delivery

368

219

View full text Add to dashboard Cite

show abstract

“…Since the first appearance of this family of nanoscale materials, their application as biocompatible carriers for a large plethora of biomedical and biotechnological applications has been explored [3][4][5]. In particular, these soft materials have gained growing interest for drug delivery because of their high loading capacity and stability [6][7][8][9][10], showing also potential use as pH and temperature sensors [11,12]. They are also employed as in vivo imaging tracers [13], in water treatment [14] and catalysis [15], as smart gating membranes for the fabrication of active photonic crystals and coatings, and for generating bioactive scaffolds in regenerative medicine [16].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of polymer nanogels by electro-Fenton process: investigation of the effect of main operation parameters

Lanzalaco

Sirés

Sabatino

et al. 2017

Electrochimica Acta

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Recently, electro-Fenton (EF) process has been shown as a promising, facile, effective, low cost and environmentally-friendly alternative for synthesizing polymer nanogels suitable as biocompatible nanocarriers for emerging biomedical applications. Here, the electrochemically-assisted modification of poly(vinylpyrrolidone) (PVP) by EF process was studied to assess the role of key operation parameters for a precise modulation of polymer crosslinking and its functionalization with [sbnd]COOH and succinimide groups. The dimensions of the nanogels, in terms of hydrodynamic radius (Rh) and weight-average molecular weight (Mw), can be tuned up by controlling the electrolysis time, current density (j) and PVP and Fe2+concentrations, as demonstrated via dynamic and static light scattering and gel permeation chromatography analysis. Using PVP at 0.25Â wt.%, Fe2+at 0.5-1.0Â mmolÂ dmâ\u88\u923and low j, short treatment times induced intramolecular crosslinking with chain scission, allowing size reduction of PVP particles from 24 to 9â\u80\u9310Â nm. Longer reaction times and higher PVP and Fe2+contents favored intermolecular crosslinking ending in Mwvalues higher than the initial 3.95Â Ã\u97Â 105Â gÂ molâ\u88\u921. An excessive [rad]OH dose from a too high circulated charge (Q), i.e., too prolonged electrolysis time even at low j or too high j even for short time, promoted intramolecular crosslinking (RhÂ â\u88¼Â 10â\u80\u9312Â nm) along with a very significant chain scission probably owing to the loss of mobility of the three-dimensional nanogel network. In conclusion, EF allowed transforming the architecture of linear, inert PVP chains into a functionalized nanogel with [sbnd]COOH and succinimide groups that have great potential for further conjugation

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“…Preliminary studies using a "non-functional" ODN have shown that the covalent bond to the nanogel does not prevent the ODN binding ability to the complementary sequence, probably due to the exibility of the particle and its link to the ODN. 27 This result was never demonstrated on a cellular model and provides motivation for the present investigation. Moreover, the ability of NG-AntimiR-31 to permeate cell membrane was investigated in the metastatic SW620 colon cancer cell line, which expresses high levels of miR-31.…”

Section: Introductionmentioning

confidence: 78%