Oligonucleotides Isolation and Separation—A Review on Adsorbent Selection

Studzińska, Sylwia; Nuckowski, Łukasz; Buszewski, Bogusław

doi:10.3390/ijms23179546

Cited by 17 publications

(3 citation statements)

References 107 publications

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“…When coupled with liquid chromatography, it achieves a selective separation of the components and a precise quantitative and qualitative determination of complex oligonucleotide mixtures. Together, they have become the primary tool for its analysis 62 . Moreover, due to the growing diversity of products and their applications, it is necessary to seek input from the global regulatory agency throughout the development on a case-by-case basis.…”

Section: Quality Controlmentioning

confidence: 99%

Antisense Oligonucleotides: Concepts and Pharmaceutical Applications

Araya,

Arias,

Coto

et al. 2023

Borneo J Pharm

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Antisense oligonucleotides are drugs whose mechanism is based on binding to RNA target sequences. For this purpose, they modify the protein expression through steric hindrance and exon omission. Its production involves several steps: synthesis, purification, and lyophilization. Usually, the most complicated procedure is synthesis due to the chemical reactions necessary to add the required oligonucleotide bases. BP1001, inotersen, nusinersen, eteplirsen, and golodirsen are a few antisense drugs developed for treating neurodegenerative and neuromuscular diseases. Although antisense oligonucleotides present off-target reactions, multiple studies are being performed. The following review shows information regarding the pharmaceutical characteristics for industrial production and the current state of applicability in clinical practice. In conclusion, some molecules have already been approved for commercialization (inotersen, nusinersen, ataluren, eteplirsen, and golodirsen), showing them as promising therapeutic solutions in the short and medium term for disorders developed by specific genetic factors.

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Section: Quality Controlmentioning

confidence: 99%

Antisense Oligonucleotides: Concepts and Pharmaceutical Applications

Araya,

Arias,

Coto

et al. 2023

Borneo J Pharm

View full text Add to dashboard Cite

show abstract

“…Unfortunately, LLE methods exhibit low recovery and limited specificity towards the target analyte [ 16 , 17 ]. Appropriate SPE processes can efficiently enrich the compounds [ 18 , 19 ]. However, commercial SPE columns usually lack active sites for the specified targets, which might reduce the separation rate and diminish reusability or specificity.…”

Section: Introductionmentioning

confidence: 99%

Highly Efficient Separation of Ethanol Amines and Cyanides via Ionic Magnetic Mesoporous Nanomaterials

Zhao,

Yang,

et al. 2024

IJMS

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Simple and efficient sample pretreatment methods are important for analysis and detection of chemical warfare agents (CWAs) in environmental and biological samples. Despite many commercial materials or reagents that have been already applied in sample preparation, such as SPE columns, few materials with specificity have been utilized for purification or enrichment. In this study, ionic magnetic mesoporous nanomaterials such as poly(4-VB)@M-MSNs (magnetic mesoporous silicon nanoparticles modified by 4-vinyl benzene sulfonic acid) and Co2+@M-MSNs (magnetic mesoporous silicon nanoparticles modified by cobalt ions) with high absorptivity for ethanol amines (EAs, nitrogen mustard degradation products) and cyanide were successfully synthesized. The special nanomaterials were obtained by modification of magnetic mesoporous particles prepared based on co-precipitation using -SO3H and Co2+. The materials were fully characterized in terms of their composition and structure. The results indicated that poly(4-VB)@M-MSNs or Co2+@M-MSNs had an unambiguous core-shell structure with a BET of 341.7 m2·g−1 and a saturation magnetization intensity of 60.66 emu·g−1 which indicated the good thermal stability. Poly(4-VB)@M-MSNs showed selective adsorption for EAs while the Co2+@M-MSNs were for cyanide, respectively. The adsorption capacity quickly reached the adsorption equilibrium within the 90 s. The saturated adsorption amounts were MDEA = 35.83 mg·g−1, EDEA = 35.00 mg·g−1, TEA = 17.90 mg·g−1 and CN−= 31.48 mg·g−1, respectively. Meanwhile, the adsorption capacities could be maintained at 50–70% after three adsorption–desorption cycles. The adsorption isotherms were confirmed as the Langmuir equation and the Freundlich equation, respectively, and the adsorption mechanism was determined by DFT calculation. The adsorbents were applied for enrichment of targets in actual samples, which showed great potential for the verification of chemical weapons and the destruction of toxic chemicals.

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“…The short length of the DNA (<100 bp) means that a spin column cannot be used to purify the polynucleotide kinase (PNK) reaction products when adding 5 phosphorylation to the primers [25]. If commercial 5 phosphorylated primers are ordered, overnight ethanol precipitation is required for PNK products with such short lengths [26]. We attempted to modify the inverse PCR-based site-directed mutagenesis protocol to avoid using 5 phosphorylated primers and compared three different kinds of DNA proofreading polymerases used for inverse PCR.…”

Section: Introductionmentioning

confidence: 99%

A Simple and Affordable Method to Create Nonsense Mutation Clones of p53 for Studying the Premature Termination Codon Readthrough Activity of PTC124

et al. 2023

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(1) Background: A premature termination codon (PTC) can be induced by a type of point mutation known as a nonsense mutation, which occurs within the coding region. Approximately 3.8% of human cancer patients have nonsense mutations of p53. However, the non-aminoglycoside drug PTC124 has shown potential to promote PTC readthrough and rescue full-length proteins. The COSMIC database contains 201 types of p53 nonsense mutations in cancers. We built a simple and affordable method to create different nonsense mutation clones of p53 for the study of the PTC readthrough activity of PTC124. (2) Methods: A modified inverse PCR-based site-directed mutagenesis method was used to clone the four nonsense mutations of p53, including W91X, S94X, R306X, and R342X. Each clone was transfected into p53 null H1299 cells and then treated with 50 μM of PTC124. (3) Results: PTC124 induced p53 re-expression in H1299-R306X and H1299-R342X clones but not in H1299-W91X and H1299-S94X clones. (4) Conclusions: Our data showed that PTC124 more effectively rescued the C-terminal of p53 nonsense mutations than the N-terminal of p53 nonsense mutations. We introduced a fast and low-cost site-directed mutagenesis method to clone the different nonsense mutations of p53 for drug screening.

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Oligonucleotides Isolation and Separation—A Review on Adsorbent Selection

Cited by 17 publications

References 107 publications

Antisense Oligonucleotides: Concepts and Pharmaceutical Applications

Antisense Oligonucleotides: Concepts and Pharmaceutical Applications

Highly Efficient Separation of Ethanol Amines and Cyanides via Ionic Magnetic Mesoporous Nanomaterials

A Simple and Affordable Method to Create Nonsense Mutation Clones of p53 for Studying the Premature Termination Codon Readthrough Activity of PTC124

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