Oligosaccharide synthesis with dextransucrase Kinetics and reaction engineering

Demuth, Kristin; Demuth, Bernd; Jördening, Hans‐Joachim; Buchholz, Klaus

doi:10.1016/s0921-0423(00)80059-3

Cited by 5 publications

(4 citation statements)

References 19 publications

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“…Fructose is formed as a by-product during this transfer and hence it can be used to monitor the process. Mostly dextransucrase is produced by Lactic Acid Bacteria (LAB) (Demuth et al 2000). Chagas et al (2007) reported that the use of cashew apple juice as substrate is an interesting alternative to grow Leuconostoc mesenteroides to produce dextransucrase.…”

Section: Prebiotic Oligosaccharidementioning

confidence: 99%

Utilization of Glycerol from Biodiesel Industry By-product into Several Higher Value Products

Mufrodi

Astuti

Budiman

et al. 2020

Applied Environmental Science and Engineering for a Sustainable Future

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material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, expressed or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Section: Prebiotic Oligosaccharidementioning

confidence: 99%

Utilization of Glycerol from Biodiesel Industry By-product into Several Higher Value Products

Mufrodi

Astuti

Budiman

et al. 2020

Applied Environmental Science and Engineering for a Sustainable Future

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“…Although there exist more detailed kinetic models for each single enzyme (Demuth et al, 2000;Zimmer, 1999), their integration is difficult due to various reasons. At first, each model has its own variances due to uncertainties, which would accumulate in the combined model.…”

Section: Kinetics and Stabilitymentioning

confidence: 99%

Co‐immobilization of dextransucrase and dextranase for the facilitated synthesis of isomalto‐oligosaccharides: Preparation, characterization and modeling

Erhardt

Kügler

Chakravarthula

et al. 2008

Biotech & Bioengineering

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Co-Immobilization of dextransucrase (DS) and dextranase (DN) into calcium alginate includes the co-entrapment of soluble DS and adsorbed DN. DS converts sucrose into dextran, which is the substrate for DN, so that isomalto-oligosaccharides (IMOs) are follow-up products of dextran hydrolysis. The boundary conditions for the successful preparation are investigated with respect to choice of DN adsorbate, surface modifications using blotting agents and optimal enzyme activity ratios. Further, repetitive batch experiments suggest the selection of medium activity ratios for continuous use (0.3 U(DN)U(-1) (DS), e.g.). Product formation at various cosubstrate:substrate concentrations as well as at different DN:DS ratios are discussed. Moreover, the complexity of the bi-enzymatic system can be reduced considering the molar ratios of cosubstrate:substrate (glucose:sucrose). Based on these factors, a mechanistic kinetic model is developed, which distinguishes the corresponding contributions of the two enzymes upon overall product formation. In general, at low glucose:sucrose ratios isomaltose synthesis is featured primarily by DN action. Yet with increasing amounts of glucose both the quantity and quality of DN substrate changes, so that its contribution to product formation decreases in an exponential manner; still the overall product yield continuously increases due to enhanced DS contribution.

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“…Based on the kinetic model of dextransucrase [5,6] the experimental data can be described with good accuracy, and it is possible to predict the initial reaction course at defined conditions. An important point for continuous operation of the enzyme is the weakly repressed dextran formation occurring with weak acceptors like glucose.…”

Section: Biocatalystmentioning

confidence: 99%

“…It was further used by Heincke et al [13] for modeling the first acceptor product in the presence of the strong acceptor maltose. Later, Demuth et al and Berensmeier et al [6,14] modeled glucose transfer to a homologous series of acceptor products:…”

Section: Modelmentioning

confidence: 99%

Integrated Enzymatic Synthesis and Adsorption of Isomaltose in a Multiphase Fluidized Bed Reactor

Ergezinger

Bohnet

Berensmeier³

et al. 2006

Engineering in Life Sciences

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Dextransucrase catalyzes the formation of dextran, but also of numerous oligosaccharides from sucrose and different acceptors, if appropriate conditions are chosen. A process on a technical scale with immobilized enzyme was established to produce isomaltose, a disaccharide of industrial interest. Isomaltose is also a reactant for dextransucrase and has to be quickly taken out of the reaction solution. This was realized by integrated adsorption of isomaltose on zeolites. In the case of biotransformation the reactor works with a fluidized bed of immobilized enzyme and the in situ separation is realized with a suspension flow of adsorbent. This process was investigated experimentally and theoretically. With a design model consisting of hydrodynamics, kinetics of enzymatic synthesis, and thermodynamics of adsorption, a comparison was made between experimental and calculated data.

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Oligosaccharide synthesis with dextransucrase Kinetics and reaction engineering

Cited by 5 publications

References 19 publications

Utilization of Glycerol from Biodiesel Industry By-product into Several Higher Value Products

Utilization of Glycerol from Biodiesel Industry By-product into Several Higher Value Products

Co‐immobilization of dextransucrase and dextranase for the facilitated synthesis of isomalto‐oligosaccharides: Preparation, characterization and modeling

Integrated Enzymatic Synthesis and Adsorption of Isomaltose in a Multiphase Fluidized Bed Reactor

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