Oligosaccharides and galactosyl cyclitols in seed desiccation tolerance

Obendorf, Ralph L.

doi:10.1017/s096025850000341x

Cited by 240 publications

(205 citation statements)

References 100 publications

Supporting

Mentioning

197

Contrasting

Unclassified

Order By: Relevance

“…The contents of total soluble sugars and of sucrose and the ratio O:S in C. echinata seeds were not correlated with the tolerance to desiccation observed for this species (Barbedo et al, 2002). According to Obendorf (1997) in orthodox seeds, the lower the mass ratio of sucrose to soluble oligosaccharides, the lower the storability. Results obtained for C. echinata suggest that the proportions of sucrose and galactosyl sucrose oligosaccharides alone do not explain its tolerance to seed desiccation.…”

Section: Resultsmentioning

confidence: 79%

“…Soluble carbohydrates are an important component involved in desiccation tolerance during seed maturation and storage (Obendorf, 1997). Raffinose, stachyose and verbascose are galactosyl sucrose oligosaccharides of the raffinose family (RFO) that rank next to sucrose in their distribution in the plant kingdom and are accumulated during seed development in a number of food and feed seed crops (Amuti & Pollard, 1977).…”

Section: Caesalpinia Echinatamentioning

confidence: 99%

“…therein). Free cyclitols and mainly galactosyl cyclitols are also accumulated in some seeds, as are raffinose and stachyose (Obendorf, 1997;Peterbauer & Richter, 2001).…”

mentioning

confidence: 99%

“…Methyl-ether derivatives of cyclitols may also form liquid crystals (Obendorf, 1997;Peterbauer & Richter, 2001).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Changes in soluble carbohydrates during storage of Caesalpinia echinata LAM. (Brazilwood) seeds, an endangered leguminous tree from the Brazilian Atlantic Forest

et al. 2006

View full text Add to dashboard Cite

Caesalpinia echinata seeds stored in laboratory environmental conditions lose their viability in one month whilst under low temperatures germination is maintained for 18 months of storage. These seeds are tolerant to desiccation, keeping their viability up to 0.08 gH 2 O.gDW -1 . Since soluble carbohydrates are believed to be involved with desiccation tolerance and seed storability, the aim of this work is to analyze the content and composition of soluble carbohydrates in C. echinata seeds during storage in paper bags (PB) and glass flasks (GF) at laboratory room (RT) and cool (CT) temperatures. In freshly harvested seeds, total soluble carbohydrates comprised approximately 10% of the dry weight, decreasing to ca. 8% over 18 months of storage at RT. In seeds stored at CT, sugars varied differently decreasing initially and being restored at the end of the analysis period. The main neutral sugars in seeds from all treatments were sucrose, fructose and glucose. Raffinose and stachyose were present as traces. Free myo-inositol and other cyclitols were also detected. The main tendency observed was the variation in levels of both glucose and fructose in relation to sucrose, the highest levels of monosaccharides which were found in seeds stored at CT. The values of glucose and fructose were practically constant in seeds stored in paper bags for 18 months at CT, decreasing consistently in the other treatments, mainly at RT. Sucrose contents remained relatively stable. Changes in soluble sugars during storage suggest that the loss of germinability of seeds of C. echinata could be associated with low levels of glucose and fructose in relation to sucrose.Keywords: seed longevity, desiccation tolerance, pernambuco, soluble sugars, cyclitols. RESUMO Modificações nos carboidratos solúveis durante o armazenamento de sementes de Caesalpinia echinata LAM. (pau-brasil), uma leguminosa arbórea da Mata Atlântica em risco de extinçãoSementes de Caesalpinia echinata (pau-brasil) perdem a viabilidade em um mês quando armazenadas no ambiente de laboratório, enquanto a capacidade germinativa é mantida quando armazenadas sob temperturas baixas. O presente trabalho teve como objetivos analisar o conteúdo e a composição dos carboidratos de sementes de C. echinata armazenadas em câmara fria (CT) e em temperatura ambiente do laboratório (RT), em duas embalagens distintas (permeável e impermeável), visando a avaliar o envolvimento desses compostos com a capacidade germinativa das sementes. Os resultados mostraram que os carboidratos solúveis são constituídos principalmente de sacarose, glicose, frutose, myo-inositol e traços de rafinose e estaquiose, totalizando cerca de 10% da massa seca das sementes. As variações nos carboidratos solúveis foram semelhantes nos dois tipos de embalagem, mas diferentes quanto à temperatura de armazenamento. Em CT, as proporções dos monossacarídeos encontradas nas sementes recém-colhidas foram mantidas por cerca de 18 meses de armazenamento, coincidindo com alta porcentagem de germinação (80%). Nas arma...

show abstract

Section: Resultsmentioning

confidence: 79%

Section: Caesalpinia Echinatamentioning

confidence: 99%

See 2 more Smart Citations

Changes in soluble carbohydrates during storage of Caesalpinia echinata LAM. (Brazilwood) seeds, an endangered leguminous tree from the Brazilian Atlantic Forest

et al. 2006

View full text Add to dashboard Cite

show abstract

“…It has been reported that the raffinose family of oligosaccharides (RFOs), which accumulate during the last phase of seed maturation, can act as "water replacement" molecules that provide the hydrogen bonds required for membrane and protein stabilization as well as for protecting DNA against hydrolytic damage (24,25). Therefore, to complement our transcriptional study, we performed carbohydrate profiling of desiccation-tolerant and -intolerant mutants using the same seed developmental stages chosen for RNA-seq analysis (15,17, and 21 DAF).…”

Section: Carbohydrate Profiling Of Seeds From Desiccation-tolerant Anmentioning

confidence: 99%

Regulatory network analysis reveals novel regulators of seed desiccation tolerance in Arabidopsis thaliana

González-Morales

Montes

Hayano-Kanashiro

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

125

100

View full text Add to dashboard Cite

Desiccation tolerance (DT) is a remarkable process that allows seeds in the dry state to remain viable for long periods of time that in some instances exceed 1,000 y. It has been postulated that seed DT evolved by rewiring the regulatory and signaling networks that controlled vegetative DT, which itself emerged as a crucial adaptive trait of early land plants. Understanding the networks that regulate seed desiccation tolerance in model plant systems would provide the tools to understand an evolutionary process that played a crucial role in the diversification of flowering plants. In this work, we used an integrated approach that included genomics, bioinformatics, metabolomics, and molecular genetics to identify and validate molecular networks that control the acquisition of DT in Arabidopsis seeds. Two DT-specific transcriptional subnetworks were identified related to storage of reserve compounds and cellular protection mechanisms that act downstream of the embryo development master regulators LEAFY COTYLEDON 1 and 2, FUSCA 3, and ABSCICIC ACID INSENSI-TIVE 3. Among the transcription factors identified as major nodes in the DT regulatory subnetworks, PLATZ1, PLATZ2, and AGL67 were confirmed by knockout mutants and overexpression in a desiccationintolerant mutant background to play an important role in seed DT. Additionally, we found that constitutive expression of PLATZ1 in WT plants confers partial DT in vegetative tissues.regulatory network | desiccation tolerance | drought tolerance | seed development | oligosaccharides D esiccation tolerance (DT) can be operationally defined as the ability of an organism to dry to equilibrium with moderately dry air (50 to 70% relative humidity at 20 to 30°C) and then resume normal function when rehydrated (1). DT organisms orchestrate a complex number of responses to protect cellular structures and prevent damage to proteins and nucleic acids. Early land plants evolved mechanisms to survive harsh drying environments to successfully exploit different ecosystems on land. Therefore, it has been postulated that the initial evolution of vegetative DT, in both vegetative and reproductive stages, was a crucial step required for the colonization of land by primitive plants of a fresh water origin (2).Seed DT, a trait that allows terrestrial plants to survive long periods of sparse water until favorable conditions are present for germination, is probably part of the answer to Darwin's "abominable mystery," the sudden appearance of great angiosperm diversity in the fossil record. In angiosperms, DT is acquired at the seed maturation stage, which involves a complex regulatory network (3, 4) that activates a large subset of genes involved in a number of mechanisms that influence seed survival in the dry state. The set of genes required for seed DT includes genes encoding protective proteins such as late embryogenesis abundant (LEA) (5, 6) and heat shock proteins (HSPs) (7), enzymes involved in scavenging reactive oxygen species (8) and the biosynthesis of protective compounds such as o...

show abstract