Oligosaccharides from Polygonatum Cyrtonema Hua: Structural characterization and treatment of LPS-induced peritonitis in mice

He, Lili; Yan, Bing-Xiong; Yao, Caiyun; Chen, Xiaoyi; Li, Longwei; Wu, Youjiao; Song, Zhijun; Song, Shanshan; Zhang, Zhifeng; Luo, Pei

doi:10.1016/j.carbpol.2020.117392

Cited by 53 publications

(36 citation statements)

References 30 publications

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“…FT‐IR spectroscopy is a tool commonly used to characterize the functional groups of carbohydrates (He et al., 2021; Zhao et al., 2020). As shown in Figure 8, RYRO1 has eight absorption bands, and the broad‐stretching intense peak near 3253 cm –1 is assigned to the stretching vibration of the hydroxyl group (He et al., 2016).…”

Section: Resultsmentioning

confidence: 99%

“…A weak‐stretching band at 2929 cm –1 is ascribed to the C–H‐stretching vibration (You et al., 2021). The peak at 1024 cm –1 is the characteristic absorption peak of asymmetric stretching vibrations of the ether bond on the sugar ring, indicating that there are pyranose units in RYRO1 (He et al., 2021; You et al., 2021). The signal at 1623 cm –1 was the characteristic absorption of bonded water, indicating the N–H of primary amine (Xiang et al., 2019).…”

Section: Resultsmentioning

confidence: 99%

“…The image at 2000 magnification shows that its surface is relatively intact and smooth, but with some folds and holes. This may be due to the low degree of branching and short chain structures of RYRO1 (He et al., 2021).…”

Section: Resultsmentioning

confidence: 99%

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Ultrasonic extraction, structural characterization, and antioxidant activity of oligosaccharides from red yeast rice

Liu

Xie

et al. 2021

Food Science & Nutrition

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Red yeast rice is consumed as a medicinal food to lower blood lipids. Besides, it is used to color food, make wine, etc. In this study, water‐soluble oligosaccharides in red yeast rice were extracted by ultrasonic‐assisted extraction method. The parameters to extract oligosaccharides from red yeast rice were optimized by the Box‐Behnken design under the following optimal extraction conditions: extraction temperature, 60°C; extraction time, 97 min; and liquid/material ratio, 25 ml/g. The structure and the antioxidant activity of the new oligosaccharide were preliminarily investigated. Total carbohydrates extracted from red yeast rice with 80% ethanol–water solution (v/v) were first removed from pigments using D101 macroporous adsorption resin. The total sugar contents were then purified by DE52 resins and Sephadex G‐25 resins to obtain red yeast rice oligosaccharides, coded as RYRO1. Structural characterization experiments indicated that RYRO1 is an oligosaccharide with a weight average molecular weight of 874 Da and a theoretical degree of polymerization of 4.86. RYRO1 is composed of mannose, glucosamine, glucose, and galactose with a molar ratio of 0.248:0.019:1:0.026. The ABTS, DPPH, and hydroxyl free radical scavenging assays showed antioxidant nature of RYRO1.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Ultrasonic extraction, structural characterization, and antioxidant activity of oligosaccharides from red yeast rice

Liu

Xie

et al. 2021

Food Science & Nutrition

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“…Previous evidence has shown that some oligosaccharides exert various biological effects, including anti-inflammatory, antioxidant capacity, and antibacterial (Sutthasupha and Lungkaphin, 2020;Wang et al, 2020;Bi et al, 2021;He et al, 2021). However, these studies were limited to that the vast majority of oligosaccharides are mixture contained oligosaccharides with different degrees of polymerization.…”

Section: Discussionmentioning

confidence: 99%

Riclinoctaose Attenuates Renal Ischemia-Reperfusion Injury by the Regulation of Macrophage Polarization

Zhao

Ding

et al. 2021

Front. Pharmacol.

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Renal ischemia-reperfusion injury is a major trigger of acute kidney injury and leads to permanent renal impairment, and effective therapies remain unresolved. Riclinoctaose is an immunomodulatory octasaccharide composed of glucose and galactose monomers. Here we investigated whether riclinoctaose protects against renal ischemia-reperfusion injury. In mice, pretreatment with riclinoctaose significantly improved renal function, structure, and the inflammatory response after renal ischemia-reperfusion. Flow cytometry analysis revealed that riclinoctaose inhibited ischemia-reperfusion-induced M1 macrophage polarization and facilitated M2 macrophage recruitment into the kidneys. In isolated mouse bone marrow-derived macrophages, pretreatment with riclinoctaose promoted the macrophage polarization toward M2-like phenotype. The inhibitor of Nrf-2/HO-1 brusatol diminished the effects of riclinoctaose on macrophage polarization. In mice, intravenous injection with riclinoctaose-pretreated bone marrow-derived macrophages also protected against renal ischemia-reperfusion injury. Fluorescence-labeled riclinoctaose specifically bound to the membrane of macrophages. Interfering with mDC-SIGN blocked the riclinoctaose function on M2 polarization of macrophages, consequently impairing the renoprotective effect of riclinoctaose. Our results revealed that riclinoctaose is a potential therapeutic agent in preventing renal ischemia-reperfusion injury.

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“…Injecting LPS into the abdominal cavity of mice can cause peritonitis and a significant increase in cytokines in mice, including IL-6, IFN-γ, IL-1β, nitric oxide (NO), and TNF-α [ 5 , 6 , 7 , 8 , 9 ]. An increasing number of therapeutic molecules have been found to be effective in attenuating LPS-mediated peritonitis.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomics Changes in the Peritoneum of Mice with Lipopolysaccharide-Induced Peritonitis

Liu

Zhang

Sun

et al. 2021

IJMS

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Peritonitis caused by LPS is a severe clinical challenge, which causes organ damage and death. However, the mechanism of LPS-induced peritonitis has not been fully revealed yet. Here, we investigated the transcriptome profile of the peritoneal tissue of LPS-induced peritonitis in mice. A model of LPS-induced peritonitis in mice was established (LPS 10 mg/kg, i.p.), and the influence of TAK 242 (TLR4 inhibitor) on the level of inflammatory cytokines in mouse peritoneal lavage fluid was investigated by using an ELISA test. Next, the peritoneal tissues of the three groups of mice (Control, LPS, and LPS+TAK 242) (n = 6) were isolated and subjected to RNA-seq, followed by a series of bioinformatics analyses, including differentially expressed genes (DEGs), enrichment pathway, protein-protein interaction, and transcription factor pathway. Then, qPCR verified-hub genes that may interact with TAK 242 were obtained. Subsequently, the three-dimensional structure of hub proteins was obtained by using homology modeling and molecular dynamics optimization (300 ns). Finally, the virtual docking between TAK 242 and hub proteins was analyzed. Our results showed that TAK 242 significantly inhibited the production of inflammatory cytokines in the peritoneal lavage fluid of mice with peritonitis, including IL-6, IFN-γ, IL-1β, NO, and TNF-α. Compared with the Control group, LPS treatment induced 4201 DEGs (2442 down-regulated DEGs and 1759 up-regulated DEGs). Compared with the LPS group, 30 DEGs were affected by TAK 242 (8 down-regulated DEGs and 22 up-regulated DEGs). A total of 10 TAK 242-triggered hub genes were obtained, and the possible docking modes between TAK 242 and hub proteins were acquired. Overall, our data demonstrated that a large number of DEGs were affected in LPS-triggered peritonitis mice. Moreover, the TLR4 inhibitor TAK 242 is capable of suppressing the inflammatory response of LPS-induced peritonitis. Our work provides clues for understanding the pathogenesis of LPS-induced peritonitis in mice.

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Oligosaccharides from Polygonatum Cyrtonema Hua: Structural characterization and treatment of LPS-induced peritonitis in mice

Cited by 53 publications

References 30 publications

Ultrasonic extraction, structural characterization, and antioxidant activity of oligosaccharides from red yeast rice

Ultrasonic extraction, structural characterization, and antioxidant activity of oligosaccharides from red yeast rice

Riclinoctaose Attenuates Renal Ischemia-Reperfusion Injury by the Regulation of Macrophage Polarization

Transcriptomics Changes in the Peritoneum of Mice with Lipopolysaccharide-Induced Peritonitis

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