Olive allergen‐specific IgE responses in patients with Olea europaea pollinosis

Quiralte, J.; Florido, Fernando; Saavedra, José Manuel Arias de; Gómez, Adalfa Chang; Pedro, B. Sáenz de San; González, Eva; Rodrı́guez, Rosalı́a

doi:10.1034/j.1398-9995.2002.057s71047.x

Cited by 25 publications

(25 citation statements)

References 15 publications

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“…4 of the 6 allergens analyzed are major allergens in this population (Ole e 1, 2, 7 and 10), and not in populations with a lower antigenic exposure. These results are in accordance with a different clinical phenotype as we previously described [8, 9]. These special characteristics together with our previous results [11,12,13] led us to analyze genetic polymorphisms related mainly to asthma and IgE regulation although the sample size was small.…”

Section: Discussionsupporting

confidence: 71%

“…Among them, Ole e 1 is the most frequent sensitizing allergen [5, 6]. Besides Ole e 1, 9 additional allergens have also been isolated and purified from O. europaea pollen extract [7], some of which, e.g., Ole e 2 and Ole e 10, are major allergens in areas with high levels of pollen in the atmosphere [8, 9]. Furthermore, our previous studies have demonstrated a strong association between Ole e 10- and Ole e 2-specific sensitization and the bronchial asthma clinical phenotype as well as relevant associations among specific sensitizations and HLA class II antigens: Ole e 2 with DR7-DQ2, and Ole e 10 with DR2(15) antigens [9].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Polymorphisms in Olive Pollen Allergy: IL13, IL4RA, IL5 and ADRB2 Genes

Llanes

Quiralte

López

et al. 2008

Int Arch Allergy Immunol

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Background: Previous results demonstrated that sensitization to specific olive pollen allergens could be related with a different clinical pattern (asthma and/or rhinitis), and that specific patterns of sensitization are regulated by different HLA class II antigens. The authors analyze the possible implication of 7 genetic polymorphisms described as asthma susceptibility genes: IL13 (C–1112T and R130Q), IL4RA (I50V, Q551R), IL5 (C–746T) and ADRB2 (Q27E and R16G) in specific olive pollen allergic sensitization. Methods: The authors genotyped seven polymorphisms of the IL13, IL4RA, IL5 and ADRB2 genes in 146 patients allergic to olive pollen with seasonal rhinitis/asthma and 50 controls using the polymerase chain reaction-restriction fragment length polymorphism and real-time polymerase chain reaction techniques. Results: Two polymorphisms of IL13 were associated with allergy to olive pollen: the TT genotype of IL13 C–1112T was decreased (odds ratio, OR = 0.35, p = 0.006) whereas the RQ heterozygous genotype of IL13 R130Q increased in patients allergic to olive pollen (OR = 3.12, p = 0.009). The combined analysis of two IL4RA single nucleotide polymorphisms (SNPs) (I50V and Q551R) showed an association with asthma: IL4RA V50/Q551 was associated with risk (OR = 2.48, p = 0.007) whereas the IL4RA V50R551 haplotype was associated with protection (OR = 0.31, p = 0.003). Conclusions: The IL13 polymorphisms under study were associated with specific allergy to olive pollen: the IL13 C–1112T polymorphism as a protective factor and the IL13 R130Q polymorphism as a risk factor. Interestingly, although single polymorphisms of IL4RA are not associated with any phenotype analyzed, the interaction between IL4RA I50V/Q551R was strongly associated with the asthma phenotype. IL13 and IL4RA could be relevant markers for allergy to olive pollen and asthma development.

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Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Analysis of Polymorphisms in Olive Pollen Allergy: IL13, IL4RA, IL5 and ADRB2 Genes

Llanes

Quiralte

López

et al. 2008

Int Arch Allergy Immunol

View full text Add to dashboard Cite

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“…19 In addition, a population of 119 sera was assayed in ELISAs and prick tests, and prevalences of 31.9% and 37.8% were found, respectively. 35 In this context, Che a 3 is an atypical polcalcin because it showed reactivity to 46% of sera from individuals with chenopod allergy. The high prevalence of this panallergen in these patients might have a relationship with their characteristic polysensitization.…”

Section: Discussionmentioning

confidence: 99%

Profilin (Che a 2) and polcalcin (Che a 3) are relevant allergens of Chenopodium album pollen: Isolation, amino acid sequences, and immunologic properties

Barderas

Villalba

Pascual³

et al. 2004

Journal of Allergy and Clinical Immunology

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“…Thus, the existence of seasonal rhinitis and/or bronchial asthma as well as a positive skin prick test to olive pollen allergen were the primary inclusion criteria for the study. The majority of olive allergic patients (ϳ90%) present associated taxonomically unrelated pollen allergies (31). However, inhibition assays of the IgE binding of olive-allergic sera to different pollen extracts suggest that O. europaea allergy should be a primary sensitization in those patients who are intensely exposed to its pollen (32).…”

Section: Discussionmentioning

confidence: 99%

A Major Allergen from Pollen Defines a Novel Family of Plant Proteins and Shows Intra- and Interspecie Cross-Reactivity

Barral

Batanero

Palomares

et al. 2004

The Journal of Immunology

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Olive tree (Olea europaea) pollen is a main cause of allergy associated with extensive areas of Europe and North America. Ole e 10, a small (10.8 kDa) and acidic (pI 5.8) protein, has been identified as a major allergen from the olive pollen, isolated, and characterized. Circular dichroism analysis gave 17% α helix, 33% β sheet, and 21% β turn for its secondary structure. Based on amino acid sequences of tryptic peptides, the protein was cloned and sequenced. The allergen consists of a single polypeptide chain of 102 aa, with a signal peptide of 21 residues. Ole e 10 showed homology with the C-terminal domain of another olive allergen, Ole e 9 (1,3-β-glucanase, 53% identity), with deduced sequences from Arabidopsis thaliana genes (42–46% identity) and with polypeptide segments (Cys boxes) of proteins involved in yeast development (Epd1/Gas-1p/Phr2 families; 42–43% similarity). Ole e 10 showed 55% prevalence for olive-allergic patients and exhibited an IgE response dependent on its conformation. Remarkable IgE cross-reactivity was detected with Ole e 9, but no correlation was observed between the individual IgE responses to both allergens. Ole e 10 shares IgE B cell epitopes with proteins from Oleaceae, Gramineae, Betulaceae, Chenopodiaceae, Cupressaceae, Ambrosia, and Parietaria pollens, latex, and vegetable foods, such as tomato, kiwi, potato, and peach. These data indicate that Ole e 10 is a new pan-allergenic plant protein that shows notable intra- and interspecie IgE cross-reactivity and is a powerful candidate to be involved in pollen-latex-fruit syndrome.

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Olive allergen‐specific IgE responses in patients with Olea europaea pollinosis

Abstract: These results indicate that Ole e 1 and Ole e 2 are major allergens in patients with O. europaea pollinosis in our population. A combination of a few olive pollen allergens can substitute the crude extract for in vivo as well as in vitro diagnostic purposes.

Cited by 25 publications

References 15 publications

Analysis of Polymorphisms in Olive Pollen Allergy: <i>IL13, IL4RA, IL5 </i>and <i>ADRB2</i> Genes

Analysis of Polymorphisms in Olive Pollen Allergy: <i>IL13, IL4RA, IL5 </i>and <i>ADRB2</i> Genes

Profilin (Che a 2) and polcalcin (Che a 3) are relevant allergens of Chenopodium album pollen: Isolation, amino acid sequences, and immunologic properties

A Major Allergen from Pollen Defines a Novel Family of Plant Proteins and Shows Intra- and Interspecie Cross-Reactivity

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