Olive leaf extract attenuates adriamycin‐induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis

Abstract

Olive (Olea europaea L.) leaf extract (OLE) possesses powerful antioxidant, antihyperlipidemic, and anti-inflammatory properties. The aim was to investigated the effects of OLE on the hyperlipidemia, antioxidant defense, heme oxygenase/biliverdin reductase (HO/BVR) pathway, inflammation, and fibrosis in spontaneously hypertensive rats with focal segmental glomerulosclerosis (FSGS, a progressive form of chronic kidney disease) induced by adriamycin (2 mg/kg, i.v., twice in a 21-day period). Daily treatment of OLE (80 mg/kg, p.o.) for 6 weeks suppressed protein oxidation and lipid peroxidation (p < .01 and p < .001, respectively), significantly increased antioxidant enzymes activities and normalized antioxidant capacity, leading to the improvement of antioxidant defense independently of the HO/BVR pathway. Furthermore, the values of triglycerides (p < .01), total, and low-density lipoprotein cholesterol (p < .05, both) were improved by OLE. OLE strongly prevented glomerulosclerosis, interstitial inflammation, and fibrosis (renal injury score, FSGS: 8 ± 0.45 vs. FSGS+OLE: 4.20 ± 1.07; p < .01), as evidenced by normalized fibronectin content (p < .001), suppressed interstitial inflammatory cells infiltration and collagen deposition, without changing cytokines expressions. OLE decreased blood pressure with a tendency to reduce urine albumin loss. These data suggest that OLE may be effective in slowing down the progression of FSGS.

Therapeutic role of Stem cells pretreated with Hyaluronic acid against chronic kidney disease in rat model

“…In the current study we did not investigate the renoprotective effect of BMSCs pretreated with HA only, but also we investigate the protective mechanisms that may include antioxidant and anti-apoptotic mechanisms. In agreement with previous study by Karanovic et al (2021) [17], our results manifested excessive concentration of lipid peroxidation and low activity of antioxidant enzymes SOD and CAT in ADR group, suggesting that ADR induced free radical formation and disturb the antioxidant defense in the kidney.…”

Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury

“…The renal tissues were homogenized in lysis buffer, as previously described [ 59 ]. Equal amounts of protein samples (concentration determined by BCA assay, Thermo Fisher scientific, USA) were separated by 12% SDS-PAGE and transferred to nitrocellulose membrane (AppliChem, Darmstadt, Germany).…”

Antioxidant Efficacy of Olive By-Product Extracts in Human Colon HCT8 Cells

“…Olive oil and olive by-products are thus a natural source of antioxidant compounds and their beneficial effects have been tested in several in vitro and in vivo experimentations [36][37][38]. Interestingly, it has been proposed that the concentration of polyphenols in the intestinal tract is higher than elsewhere because phenols can reach the colon directly or through the bile.…”