Olive oil as an alternative to boar semen cryopreservation

Silva, Estela Fernandes e; Cardoso, Tainã Figueiredo; Dutra, Fabiana Lemos Goulart; Varela, Antônio Sérgio; Corcini, Carine Dahl

doi:10.21615/cesmvz.11.1.1

Cited by 2 publications

(1 citation statement)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To protect sperm cells from the oxidative stress induced by free radicals, a robust array of antioxidants and fatty acids has been used in semen cryopreservation. It has been reported earlier that those fatty acids from various sources, such as DHA from fish oil [ 11 – 13 ], DHA from different egg yolks, and olive oil, [ 14 ] influence fatty acids on the sperm plasma membrane and consequently improve post-thawed boar semen quality [ 15 ]. Besides supplementation of fatty acid into freezing extender, palm kernel meal protein hydrolysate with its bioactive peptides [ 16 , 17 ], curcumin [ 18 ], and resveratrol [ 19 ] have also been applied to improve frozen boar semen quality, such as total motility, progressive motility and viability as well as minimize lipid peroxidation.…”

Section: Introductionmentioning

confidence: 99%

Camellia oil with its rich in fatty acids enhances post-thawed boar sperm quality

Khophloiklang,

Chanapiwat,

Kaeoket

2024

Acta Vet Scand

View full text Add to dashboard Cite

Background Boar sperm are highly susceptible to specific conditions during cryopreservation, leading to a significant decrease in their fertilizing potential due to damage to their membranes. Camellia oil, known for its fatty acids with antioxidant and biological properties, has not been previously explored for the cryopreservation of boar semen. This study aimed to examine the effects of camellia oil on post-thawed boar sperm quality. Boar semen ejaculates (n = 9) were collected and divided into six equal aliquots based on camellia oil concentrations (0, 0.5, 1, 1.5, 2 and 2.5% v/v) in the freezing extender. Semen samples were processed and cryopreserved using the liquid nitrogen vapor method. Thereafter, frozen semen samples were thawed at 50 °C for 12 s and evaluated for sperm morphology by scanning electron microscope, sperm motility using a computer-assisted sperm analyzer, sperm viability, acrosome integrity, mitochondrial function, MDA level and total antioxidant capacity. Results The results demonstrated that the supplementation of 1.5% (v/v) camellia oil showed superior post-thaw sperm qualities such as improved sperm morphology, motility, acrosome integrity and mitochondrial function by 14.3%, 14.3% and 11.7%, respectively, when compared to the control group. Camellia oil at a concentration of 1.5% (v/v) showed the lowest level of MDA (18.3 ± 2.1 µmol/L) compared to the other groups. Conclusions In conclusion, adding 1.5% (v/v) camellia oil in the freezing extender reduced the oxidative damage associated with cryopreservation and resulted in a higher post-thawed sperm quality.

show abstract

Section: Introductionmentioning

confidence: 99%

Camellia oil with its rich in fatty acids enhances post-thawed boar sperm quality

Khophloiklang,

Chanapiwat,

Kaeoket

2024

Acta Vet Scand

View full text Add to dashboard Cite

show abstract

Crude Garden Cress Seed Oil (Lepidium sativum Linn.) Enhances Post-Thawed Boar Sperm Quality

Khophloiklang,

Chanapiwat,

Kaeoket

2024

Animals

View full text Add to dashboard Cite

This study aimed to examine the effects of crude garden cress seed oil (CGCSO) on frozen–thawed boar sperm qualities. Semen ejaculates (n = 12) were collected and further divided into six equal aliquots based on CGCSO concentrations (0, 0.5, 1, 1.5, 2, and 2.5% v/v) in the freezing extender. Semen samples were processed and cryopreserved utilizing the traditional liquid nitrogen vapor technique. Subsequently, semen samples were thawed in a thermos with warm water at 50 °C for 12 s and evaluated for sperm morphology using scanning electron microscopy, sperm motility using a CASA, sperm viability, acrosome integrity, mitochondrial function, MDA level, total antioxidant capacity (TAC), glutathione peroxidase (GSH-Px), and catalase (CAT) activity. The results indicated that 1% CGCSO resulted in superior post-thaw sperm characteristics, including enhanced sperm morphology, motility, viability, acrosome integrity, and mitochondrial function. Particularly, the total motile sperm increased by 16.5%, progressive motile sperm increased by 13.0%, viability improved by 15.1%, acrosome integrity increased by 14%, and mitochondrial function improved by 14.1% compared to the control group. CGCSO treatment at 1% and 1.5% exhibited the lowest level of MDA (45.73 ± 11.2 and 45.73 ± 11.3 µmol/L, respectively) compared to the other groups. The CGCSO-supplemented groups showed higher values of TAC, GSH-Px, and CAT than the control group but not significantly.

show abstract

Olive oil as an alternative to boar semen cryopreservation

Cited by 2 publications

References 40 publications

Camellia oil with its rich in fatty acids enhances post-thawed boar sperm quality

Camellia oil with its rich in fatty acids enhances post-thawed boar sperm quality

Crude Garden Cress Seed Oil (Lepidium sativum Linn.) Enhances Post-Thawed Boar Sperm Quality

Contact Info

Product

Resources

About