Olive (olea europea) pollen allergens—I immunochemical characterization by immunoblotting, crie and immunodetection by a monoclonal antibody

An olive allergen‐like protein has been isolated from lilac (Syringa vulgaris) pollen extract. The protein can be considered as an allergen since is recognized by IgE from olive hypersensitive human sera, and has been called Syr v I (IUIS nomenclature). This protein consists of a glycosylated polypeptide of 20 kDa, which has an amino acid composition, spectroscopic properties, and an N‐terminal sequence similar to the major allergen from olive pollen, Ole e I. The lilac allergen is recognized by rabbit polyclonal antisera raised against olive allergen as well as by an Ole e I‐specific monoclonal antibody. Using a polymerase chain reaction strategy, based on the similarities observed between these olive and lilac proteins, three cDNA clones encoding Syr v I have been isolated and sequenced. These clones code for a polymorphic protein of 145 residues with a derived molecular mass of about 16400Da, which contains a potential N‐glycosylation site. Comparison of the deduced amino acid sequences of these Syr v I isoforms to each other revealed identities of 90–97%. Moreover, these sequences showed a high degree of similarity (85.5–89.6% identity) with Ole e I. The structural and immunological characterization of Syr v I justify the cross‐reactions observed between olive and lilac pollen extracts. The molecular cloning of Syr v I is relevant for the epitope mapping in Oleacease allergens, and may contribute to an improvement in the design of reagents for diagnosis and therapy of IgE‐dependent allergic reactions.

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“…2b) suggests that this Asnlll is glycosylated. This residue has been demonstrated to be glycosylated in the olive allergen [14].…”

Section: Isolation Of Total Rna From Lilac Pollen Cdna Preparation mentioning

confidence: 99%

“…The Ole e I-specific monoclonal antibody 19G6 was kindly donated by Dr Lahoz. It was obtained by immunizing eight BALB/c mice with olive pollen crude extract as previously described [14].…”

Section: Electrophoresis Immunoblotting and Carbohydrate Detectionmentioning

confidence: 99%

Isolation and characterization of an olive allergen‐like protein from lilac pollen

Batanero

Villalba

López-Otı́n

et al. 1994

European Journal of Biochemistry

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“…The distribution of Ole e 1 isoforms on 2-D polyacrylamide gels was studied using an antiOle e 1 antibody, namely 10H1 (Lauzurica et al 1988). This antibody was able to recognize up to 12 different immunoreactive protein spots in the molecular weight range of 18-22 kDa ( Fig.…”

Section: Dilution Secondary Antibody (Source) Dilutionmentioning

confidence: 99%

“…Ole e 1 Mouse anti-olive Ole e 1 mAb (Lauzurica et al 1988) 1:5,000 Goat anti-mouse IgG Ab, DyLight 633-conjugated (Agrisera, Vännäs, Sweden)…”

Section: Dilution Secondary Antibody (Source) Dilutionmentioning

confidence: 99%

Detection and Quantitation of Olive Pollen Allergen Isoforms Using 2-D Western Blotting

Zienkiewicz¹,

García-Quirós²,

Alché³

et al. 2012

Current Insights in Pollen Allergens

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“…Immunoblots were performed independently in the case of Ole e 1 ( The membranes were probed with antibodies to the following allergenic proteins: Ole e 1, (olive pollen major allergen), Ole e 2 (profilin), Ole e 5 (Cu,Zn superoxide dismutase) and Ole e 9 (1,3-β-glucanase). The anti-Ole e 1 mAb was kindly provided by Dr. Carlos Lahoz (Fundación Jiménez Díaz, Madrid, Spain) (Lauzurica et al 1988). The anti-Ole e 2 polyclonal antibody (PoAb) was produced by immunization of rabbits with a keyhole limpet hemocyanin (KLH)-linked synthetic peptide (AQSATFPQFKPEEM) designed from the predicted amino acid sequence of an olive profilin (Ole e 2).…”

Section: Immunoblottingmentioning

confidence: 99%

Clustering of Olive Pollens into Model Cultivars on the Basis of Their Allergenic Content

Morales¹,

Castro²,

Salmerón³

et al. 2012

Current Insights in Pollen Allergens

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Olive (olea europea) pollen allergens—I immunochemical characterization by immunoblotting, crie and immunodetection by a monoclonal antibody

Cited by 62 publications

References 31 publications

Isolation and characterization of an olive allergen‐like protein from lilac pollen

Isolation and characterization of an olive allergen‐like protein from lilac pollen

Detection and Quantitation of Olive Pollen Allergen Isoforms Using 2-D Western Blotting

Clustering of Olive Pollens into Model Cultivars on the Basis of Their Allergenic Content

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