We conducted a series of hydroxylation experiments using mm-sized cuboids cut from six different crystals of San Carlos olivine with a range of trace-element concentrations. The cuboids were pre-annealed and then hydroxylated under identical conditions, ensuring that variation in the amounts of H incorporated depended only on the compositional variables. The pre-anneal was at 1400 °C, atmospheric pressure and an oxygen fugacity equivalent to Δlog FMQ + 1, with the subsequent hydroxylation at 800 °C and 1.5 GPa, for 3 days. Hydrogen was incorporated into all six crystals by the four main substitution mechanisms [Si], [Mg], [Ti] and [triv], with homogeneous H contents in the cores of the crystals, indicating H diffusion rates faster than 10 − 11 m 2 /s. Total H as H 2 O in the homogeneous cores calculated by summing all the infrared absorbance bands ranges from 13 to 27 wt. ppm. The total H 2 O in the six pre-annealed crystals is poorly correlated with any measured compositional variable. However, when the H 2 O associated with individual infrared bands is compared, clear trends emerge. The intensity of absorption bands at 3572 and 3525 cm − 1 are strongly correlated with Ti concentrations, whose range in the six crystals exceeds an order of magnitude. Bands between 3400 and 3300 cm − 1 , correlate negatively with Na + , but are positively correlated with the difference between molar Cr 3+ and Na + . This highlights a previously unrecognised role for Na in suppressing H incorporation in natural olivines. The results confirm the important role that the trace constituents of olivine play in H incorporation. Two of these trace elements, Na and Ti, tend to be similarly enriched or depleted by partial melting or metasomatism of the mantle, but have opposite effects on H incorporation, with Ti enhancing it but Na suppressing it. Models estimating the effect of H in olivine on mantle rheology must, therefore, consider carefully the availability of these trace elements.