Omega-3- and Resveratrol-Loaded Lipid Nanosystems for Potential Use as Topical Formulations in Autoimmune, Inflammatory, and Cancerous Skin Diseases

Abstract

Resveratrol (RSV) and omega 3 (ω3), because of their biological favorable properties, have become subjects of interest for researchers in dermocosmetic and pharmaceutical industries; however, these bioactives present technological limitations that hinder their effective delivery to the target skin layer. To overcome the stability and skin permeation limitations of free bioactives, this work proposes a combined strategy involving two different lipid nanosystems (liposomes and lipid nanoparticles) that include ω3 in their lipid matrix. Additionaly, RSV is only encapsulated in liposomes that provid an adequate amphiphilic environment. Each formulation is thoroughly characterized regarding their physical–chemical properties. Subsequently, the therapeutic performance of the lipid nanosystems is evaluated based on their protective roles against lipid peroxidation, as well as inhibition of cicloxygenase (COX) and nitric oxid (NO) production in the RWA264.7 cell line. Finally, the lipid nanosystems are incorporated in hydrogel to allow their topical administration, then rheology, occlusion, and RSV release–diffusion assays are performed. Lipid nanoparticles provide occlusive effects at the skin surface. Liposomes provide sustained RSV release and their flexibility conferred by edge activator components enhances RSV diffusion, which is required to reach NO production cells and COX cell membrane enzymes. Overall, the inclusion of both lipid nanosystems in the same semisolid base constitutes a promising strategy for autoimmune, inflammatory, and cancerous skin diseases.

Resveratrol-Loaded Levan Nanoparticles Produced by Electrohydrodynamic Atomization Technique

“…Then, a linear standard calibration curve was constructed by plotting the graph of the absorbance at 309 nm of RS against concentration (R 2 = 0.99) for the quantitative determination of release data ( Figure 5 b). The release of RS from nanoparticles was carried out in PBS pH 7.4 to mimic the physiological conditions in the body [ 33 ], and pH 5.0 to mimic the natural environment of skin and wound [ 53 , 63 , 64 ] at a controlled temperature of 37 °C. In Figure 5 c), a burst effect was observed in all formulations.…”

pH-Responsive Hybrid Nanoassemblies for Cancer Treatment: Formulation Development, Optimization, and In Vitro Therapeutic Performance

“…Encapsulation efficiency (EE) was determined by ultrafiltration technique [ 35 ]. In brief, DOX-LNLCs previously diluted in water (1:2, v/v ) were transferred to filter units with 50 kDa pores (Amicon ® Ultra-15, Millipore Corporation, Burlington, MA, USA) and centrifuged at 3000 rpm for 10 min (Hettich ® Universal 320 centrifuge, Kirchlengern, Germany).…”

“…In vitro drug release studies were performed by the dialysis method [ 35 ]. DOX-LNLCs (1.0 mL) with DOX concentration of 0.03 mg/mL were added to dialysis membranes (Float-A-Lyzer ® , 3.5 kD, VWR International, Radnor, PA, USA) that were immersed in 40 mL of dissolution medium, ensuring sink conditions.…”

Modulating Inflammation-Mediated Diseases via Natural Phenolic Compounds Loaded in Nanocarrier Systems

“…An optimized co-loading of resveratrol and omega-3 (ω3) in lipid nanosystem has strengthened their anti-inflammatory potential in skin lesion diseases. The said formulation displayed longer circulation time and better inhibition of COX-2 and NO production in LPS-induced cells [ 129 ]. The incorporation of resveratrol in peptide hydrogel also boosted its wound-healing effectiveness by hindering the release of macrophage cytokines and promoting collagen restructuration [ 130 ].…”