Omega-3-fatty acid adds to the protective effect of flax lignan concentrate in pressure overload-induced myocardial hypertrophy in rats via modulation of oxidative stress and apoptosis

Ghule, Arvindkumar E.; Kandhare, Amit D.; Jadhav, Suresh; Zanwar, Anand A.; Bodhankar, Subhash L.

doi:10.1016/j.intimp.2015.08.005

Cited by 55 publications

(33 citation statements)

References 79 publications

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“…Reactive Oxygen Species (ROS) production was quantified by using dichlorodihydrofluorescein diacetate (H 2 DCFDA) dye method based on the ROS-dependent oxidation of DCFH-DA to DCF according to the method described elsewhere (Ghule et al, 2015[26]). …”

Section: Methodsmentioning

confidence: 99%

Neuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in rats: evidence from anti-inflammatory, antioxidant and anti-apoptotic studies

Kandhare

Mukherjee

Bodhankar

2017

EXCLI Journal; 16:Doc546; ISSN 1611-2156

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Section: Methodsmentioning

confidence: 99%

Neuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in rats: evidence from anti-inflammatory, antioxidant and anti-apoptotic studies

Kandhare

Mukherjee

Bodhankar

2017

EXCLI Journal; 16:Doc546; ISSN 1611-2156

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“…The limb leads were placed on the right forelimb (negative electrode), left forelimb (positive electrode) and right hind leg (neutral electrode). Electrocardiographic changes were recorded using an 8-channels Power Lab System (ADInstruments) [30]. …”

Section: Methodsmentioning

confidence: 99%

The effects of a 50-Hz magnetic field on the cardiovascular system in rats

Zhou¹,

Wan²,

Liu³

et al. 2016

Journal of Radiation Research

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A 50-Hz magnetic field (MF) is a potential health-risk factor. Its effects on the cardiovascular system have not been fully investigated. This study was conducted to explore the effects of long-term exposure to a 50-Hz MF on the cardiovascular system. In the study, an exposure system was constructed, and the distribution of the 50-Hz MF was determined. Sixty-four Sprague-Dawley (SD) rats were exposed to a 50-Hz MF at 100 μT for 24 weeks, 20 h per day, while another 64 rats were sham exposed. During the exposure, blood pressure was measured every 4 weeks. After 24 weeks, echocardiography, cardiac catheterization and electrocardiography were performed. Moreover, heart and body weight were recorded, and haematoxylin–eosin staining and real-time PCR were conducted. The results showed that compared with the sham group, exposure to a 50-Hz MF did not exert any effects on blood pressure, pulse rate, heart rate or cardiac rhythm. Furthermore, echocardiography and cardiac catheterization showed that there were no significant differences in the cardiac morphology or haemodynamics. In addition, histopathological examination showed that exposure to a 50-Hz MF had no effects on the structure of the heart. Finally, expression of the cardiac hypertrophy–related genes did not show any significant differences between the 50-Hz MF exposure group and the sham group. Taken together, in SD rats, exposure to a 50-Hz/100 μT MF for 24 weeks did not show any obvious effects on the cardiovascular system.

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“…Studies have shown that ω‐3 PUFAs can protect cells against apoptosis; including smooth muscle cells, cardiomyocytes during pressure overload‐induced cardiac hypertrophy, and neuronal apoptosis following hyperoxic injury . Therefore, ω‐3 PUFAs may have anti‐apoptotic effects on cardiomyocytes following MI.…”

Section: Introductionmentioning

confidence: 99%

“…9,10 Reversal of cardiomyocyte apoptosis during early stage of MI is crucial for maintaining cardiac function. 11 Studies have shown that ω-3 PUFAs can protect cells against apoptosis; including smooth muscle cells, 12 cardiomyocytes during pressure overload-induced cardiac hypertrophy, 13 and neuronal apoptosis following hyperoxic injury. 14 proliferation, and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Modulation of apoptosis‐related microRNAs following myocardial infarction in fat‐1 transgenic mice vs wild‐type mice

Chen

Sang

et al. 2018

J Cellular Molecular Medi

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BackgroundmicroRNAs (miRNAs) post‐transcriptionally regulate cardiac repair following myocardial infarction (MI). Omega‐3 polyunsaturated fatty acid (ω‐3 PUFAs) may support cardiac healing after MI, but the mechanism is unclear.MethodsThe fat‐1 transgenic mouse expresses a ω‐3 fatty acid desaturase which converts ω‐6 PUFAs to ω‐3 PUFAs in vivo. MI was induced in fat‐1 transgenic (n = 30) and wild‐type (WT) mice (n = 30) using permanent ligation. Other transgenic and WT mice underwent sham procedure (n = 30 and n = 30, respectively). One week after occlusion, cardiac function was measured by echocardiography and the infarct size was assessed using histology and miRNA microarray profiling. Expression of selected miRNA was confirmed using quantitative real‐time PCR.ResultsOne week following MI, the fat‐1 transgenic myocardium had better cardiac function, a smaller fibrotic area, and fewer apoptotic cardiomyocytes than WT myocardium. Post‐MI profiling showed 33 miRNAs that were significantly up‐regulated, and 35 were down‐regulated, in fat‐1 group compared to the WT group (n = 3 and n = 2 mice, respectively). Among selected apoptosis‐associated miRNAs, 9 miRNAs were up‐regulated (miR‐101a‐3p, miR‐128‐3p,miR‐133a‐5p,miR‐149‐5p,miR‐192‐5p,miR‐1a‐3p,miR‐208a‐3p,miR‐29c‐5p,miR‐30c‐2‐3p), and 3 were down‐regulated (miR‐210‐3p,miR‐21a‐3p,miR‐214‐3p) in fat‐1 transgenic mice compared with WT mice. Kyoto encyclopaedia of genes and genomes (KEGG) pathway analysis indicated likely roles for these miRNAs in MI. Furthermore, Bcl‐2 expression was increased, and caspase‐3 decreased, in infarcted fat‐1 transgenic mouse hearts compared to WT hearts.Conclusionsω‐3 PUFAs may have a protective effect on cardiomyocytes following MI through their modulation of apoptosis‐related miRNAs and target genes.

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Omega-3-fatty acid adds to the protective effect of flax lignan concentrate in pressure overload-induced myocardial hypertrophy in rats via modulation of oxidative stress and apoptosis

Cited by 55 publications

References 79 publications

Neuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in rats: evidence from anti-inflammatory, antioxidant and anti-apoptotic studies

Neuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in rats: evidence from anti-inflammatory, antioxidant and anti-apoptotic studies

The effects of a 50-Hz magnetic field on the cardiovascular system in rats

Modulation of apoptosis‐related microRNAs following myocardial infarction in fat‐1 transgenic mice vs wild‐type mice

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