Omega-3 fatty acid production from enzyme saccharified hemp hydrolysate using a novel marine thraustochytrid strain

Gupta, Adarsha; Abraham, Reinu E.; Barrow, Colin J.; Puri, Munish

doi:10.1016/j.biortech.2014.11.031

Cited by 40 publications

(9 citation statements)

References 35 publications

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“…Similarly, it is predicted that the growth of thraustochytrid cells in fructose or glycerol would have led to the complete consumption of the carbon source added initially. The growth rates achieved in this study are higher than the published productivities for most of the characterized members of the thraustochytrids family [2,10,21,22]. However, higher growth rates, 26 gDW/L and 55 gDW/L, were documented when cultures were batch-fed with higher concentrations of the carbon source [23,24].…”

Section: Biomass Productioncontrasting

confidence: 70%

The Nutritional and Pharmacological Potential of New Australian Thraustochytrids Isolated from Mangrove Sediments

et al. 2020

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Mangrove sediments represent unique microbial ecosystems that act as a buffer zone, biogeochemically recycling marine waste into nutrient-rich depositions for marine and terrestrial species. Marine unicellular protists, thraustochytrids, colonizing mangrove sediments have received attention due to their ability to produce large amounts of long-chain ω3-polyunsaturated fatty acids. This paper represents a comprehensive study of two new thraustochytrids for their production of valuable biomolecules in biomass, de-oiled cakes, supernatants, extracellular polysaccharide matrixes, and recovered oil bodies. Extracted lipids (up to 40% of DW) rich in polyunsaturated fatty acids (up to 80% of total fatty acids) were mainly represented by docosahexaenoic acid (75% of polyunsaturated fatty acids). Cells also showed accumulation of squalene (up to 13 mg/g DW) and carotenoids (up to 72 µg/g DW represented by astaxanthin, canthaxanthin, echinenone, and β-carotene). Both strains showed a high concentration of protein in biomass (29% DW) and supernatants (2.7 g/L) as part of extracellular polysaccharide matrixes. Alkalinization of collected biomass represents a new and easy way to recover lipid-rich oil bodies in the form of an aqueous emulsion. The ability to produce added-value molecules makes thraustochytrids an important alternative to microalgae and plants dominating in the food, pharmacological, nutraceutical, and cosmetics industries.

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Section: Biomass Productioncontrasting

confidence: 70%

The Nutritional and Pharmacological Potential of New Australian Thraustochytrids Isolated from Mangrove Sediments

et al. 2020

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“…A suitable method for the use of the hydrolysed sugars for producing omega-3 fatty acids has been developed in a previous study. Our results indicated that these sugars are suitable for growing marine microalgae for producing bioactives [44]. The yield of reducing sugars lowered in samples obtained after sequential alkaline and acid treatment.…”

Section: Resultsmentioning

confidence: 87%

Enrichment of Cellulosic Waste Hemp (Cannabis sativa) Hurd into Non-Toxic Microfibres

2016

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In this study a largely available lignocellulose feedstock hemp (Cannabis sativa), obtained as an industrial waste, was used for cellulose extraction. The extraction of cellulose microfibres from hemp biomass was conducted by alkaline treatment and an acidification process. The extracted cellulose microfibres were characterised using Fourier-transformed infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and X-ray diffraction (XRD). The viability of the study was determined by growing human fibroblasts on the preparation which resulted in being non-toxic; indicating its potential in preparing biological scaffolds. Upon enzymatic hydrolysis of the cellulose microfibre using cellulase from Trichoderma reesei, a maximum of 909 mg/g of reducing sugars were obtained, which endorses its suitability for biofuel production.

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“…Microbial oils can potentially augment fossil fuel use, since over the long term fuel supply is unsustainable . Additionally, some of these microbial oils contain polyunsaturated fatty acids (PUFAs), particularly omega‐3 fatty acids that offer a more controllable and renewable replacement for omega‐3s from fish oil .…”

Section: Introductionmentioning

confidence: 99%

Exploring omega‐3 fatty acids, enzymes and biodiesel producing thraustochytrids from Australian and Indian marine biodiversity

Gupta

Singh

Byreddy

et al. 2015

Biotechnology Journal

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The marine environment harbours a vast diversity of microorganisms, many of which are unique, and have potential to produce commercially useful materials. Therefore, marine biodiversity from Australian and Indian habitat has been explored to produce novel bioactives, and enzymes. Among these, thraustochytrids collected from Indian habitats were shown to be rich in saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs), together constituting 51-76% of total fatty acids (TFA). Indian and Australian thraustochytrids occupy separate positions in the dendrogram, showing significant differences exist in the fatty acid profiles in these two sets of thraustochytrid strains. In general, Australian strains had a higher docosahexaenoic acid (DHA) content than Indian strains with DHA at 17-31% of TFA. A range of enzyme activities were observed in the strains, with Australian strains showing overall higher levels of enzyme activity, with the exception of one Indian strain (DBTIOC-1). Comparative analysis of the fatty acid profile of 34 strains revealed that Indian thraustochytrids are more suitable for biodiesel production since these strains have higher fatty acids content for biodiesel (FAB, 76%) production than Australian thraustochytrids, while the Australian strains are more suitable for omega-3 (40%) production.

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Omega-3 fatty acid production from enzyme saccharified hemp hydrolysate using a novel marine thraustochytrid strain

Cited by 40 publications

References 35 publications

The Nutritional and Pharmacological Potential of New Australian Thraustochytrids Isolated from Mangrove Sediments

The Nutritional and Pharmacological Potential of New Australian Thraustochytrids Isolated from Mangrove Sediments

Enrichment of Cellulosic Waste Hemp (Cannabis sativa) Hurd into Non-Toxic Microfibres

Exploring omega‐3 fatty acids, enzymes and biodiesel producing thraustochytrids from Australian and Indian marine biodiversity

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