Omega-3 Fatty Acids Produced from Microalgae

Puri, Munish; Thyagarajan, T. R.; Gupta, Adarsha; Barrow, Colin J.

doi:10.1007/978-3-642-53971-8_45

Cited by 4 publications

(4 citation statements)

References 116 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is therefore a need to find novel and sustainable sources of these bioactive compounds. In this sense, microalgae are considering potential sustainable sources of n-3 LC-PUFAs [ 11 , 12 ] due to their advantages over terrestrial crops such as higher growth rate, higher biomass production and smaller cultivation area [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Advanced Extraction of Lipids with DHA from Isochrysis galbana with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions

2020

View full text Add to dashboard Cite

Microalgal biomass is a sustainable and valuable source of lipids with omega-3 fatty acids. The efficient extraction of lipids from microalgae requires fast and alternative extraction methods, frequently combined with biomass pre-treatment by different procedures. In this work, Pressurized liquid extraction (PLE) was optimized and compared with traditional lipid extraction methods, Folch and Bligh and Dyer, and with a new Ultrasound Assisted Extraction (UAE) method for lipids from microalgae Isochrysis galbana. To further optimize PLE and UAE, enzymatic pre-treatment of microalga Isochrysis galbana was studied with commercial enzymes Viscozyme and Celluclast. No significant differences were found for lipid yields among different extraction techniques used. However, advanced extraction techniques with or without pre-treatment are a green, fast, and toxic solvent free alternative to traditional techniques. Lipid composition of Isochrysis was determined by HPLC-ELSD and included neutral and polar lipids, showing that each fraction comprised different contents in omega-3 polyunsaturated fatty acids (PUFA). The highest polar lipids content was achieved with UAE (50 °C and 15 min) and PLE (100 °C) techniques. Moreover, the highest omega-3 PUFA (33.2%), eicosapentaenoic acid (EPA) (3.3%) and docosahexaenoic acid (DHA) (12.0%) contents were achieved with the advanced technique UAE, showing the optimized method as a practical alternative to produce valuable lipids for food and nutraceutical applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Advanced Extraction of Lipids with DHA from Isochrysis galbana with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions

2020

View full text Add to dashboard Cite

show abstract

“…Because fish obtain most PUFAs from phytoplankton (5), the production of PUFAs by microalgae has been studied for decades. Many algae strains can produce DHA and EPA (6), and some eukaryotic microalgae, such as Botryococcus braunii or Nannochloropsis sp., can accumulate up to 70% of lipids in their biomass (7). Marine microalgae, such as Nannochloropsis sp.…”

Section: Introductionmentioning

confidence: 99%

“…Of all marine microalgae, thraustochytrids, strains from the order Thraustochytrida are considered to be the most suitable candidates for PUFA production (6). During heterotrophic cultivation, Thraustochytrida can accumulate PUFAs at rates as high as 10 g L −1 d −1 (10), which is significantly higher compared with other microalgae such as Nannochloropsis (11) or Tisochrysis (12) during autotrophic or mixotrophic cultivation, as shown in Supplementary Table S1.…”

Section: Introductionmentioning

confidence: 99%

Marine microalgae Schizochytrium demonstrates strong production of essential fatty acids in various cultivation conditions, advancing dietary self-sufficiency

Literáková,

Zavřel,

Búzová

et al. 2024

Front. Nutr.

View full text Add to dashboard Cite

IntroductionPolyunsaturated fatty acids (PUFAs) are essential nutrients that humans obtain from their diet, primarily through fish oil consumption. However, fish oil production is no longer sustainable. An alternative approach is to produce PUFAs through marine microalgae. Despite the potential of algae strains to accumulate high concentrations of PUFAs, including essential fatty acids (EFAs), many aspects of PUFA production by microalgae remain unexplored and their current production outputs are frequently suboptimal.MethodsIn this study, we optimized biomass and selected ω-3 PUFAs production in two strains of algae, Schizochytrium marinum AN-4 and Schizochytrium limacinum CO3H. We examined a broad range of cultivation conditions, including pH, temperature, stirring intensity, nutrient concentrations, and their combinations.ResultsWe found that both strains grew well at low pH levels (4.5), which could reduce bacterial contamination and facilitate the use of industrial waste products as substrate supplements. Intensive stirring was necessary for rapid biomass accumulation but caused cell disruption during lipid accumulation. Docosahexaenoic acid (DHA) yield was independent of cultivation temperature within a range of 28–34°C. We also achieved high cell densities (up to 9 g/L) and stable DHA production (average around 0.1 g/L/d) under diverse conditions and nutrient concentrations, with minimal nutrients required for stable production including standard sea salt, glucose or glycerol, and yeast extract.DiscussionOur findings demonstrate the potential of Schizochytrium strains to boost industrial-scale PUFA production and make it more economically viable. Additionally, these results may pave the way for smaller-scale production of essential fatty acids in a domestic setting. The development of a new minimal culturing medium with reduced ionic strength and antibacterial pH could further enhance the feasibility of this approach.

show abstract

“…However, there are some issues with using microorganisms in animal diets due to low digestibility, heavy metals, and toxicity [ 12 ]. Although the cell wall is non-digestible in pigs, bioactive components could be extracted to enhance intestinal health in pigs and broilers [ 59 , 60 , 61 ].…”

Section: Introductionmentioning

confidence: 99%

Use of Microorganisms as Nutritional and Functional Feedstuffs for Nursery Pigs and Broilers

Cheng

Kim

2022

Animals

View full text Add to dashboard Cite

The objectives of this review paper are to introduce the structures and composition of various microorganisms, to show some applications of single cells as alternative protein supplements or energy feeds in swine and poultry diets, and to discuss the functional effects of microorganisms as feed additives on the growth performance and intestinal health of nursery pigs and broilers. Microorganisms, including bacteria, yeasts, and microalgae, have been commonly supplemented in animal diets because they are cost-effective, stable, and have quantitative production that provides nutritional and functional benefits to pigs and broilers. Microorganisms could be alternative antibiotics to enhance intestinal health due to bioactive components from cell wall components, which interact with receptors on epithelial and immune cells. In addition, bioactive components could be digested by intestinal microbiota to produce short-chain fatty acids and enhance energy utilization. Otherwise, microorganisms such as single-cell protein (SCP) and single-cell oils (SCOs) are sustainable and economic choices to replace conventional protein supplements and energy feeds. Supplementing microorganisms as feedstuffs and feed additives improved the average daily gain by 1.83%, the daily feed intake by 0.24%, and the feed efficiency by 1.46% in pigs and broilers. Based on the properties of each microorganism, traditional protein supplements, energy feeds, and functional feed additives could be replaced by microorganisms, which have shown benefits to animal’s growth and health. Therefore, specific microorganisms could be promising alternatives as nutritional and functional feedstuffs in animal diets.

show abstract

Omega-3 Fatty Acids Produced from Microalgae

Cited by 4 publications

References 116 publications

Advanced Extraction of Lipids with DHA from Isochrysis galbana with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions

Advanced Extraction of Lipids with DHA from Isochrysis galbana with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions

Marine microalgae Schizochytrium demonstrates strong production of essential fatty acids in various cultivation conditions, advancing dietary self-sufficiency

Use of Microorganisms as Nutritional and Functional Feedstuffs for Nursery Pigs and Broilers

Contact Info

Product

Resources

About