Omega-3 polyunsaturated fatty acids inhibit the increase in cytokines and chemotactic factors induced in vitro by lymph fluid from an intestinal ischemia-reperfusion injury model

Zhang, Rui; He, Guizhen; Wang, Yu-kang; Zhou, Kai-guo; Ma, Enlong

doi:10.1016/j.nut.2014.10.015

Cited by 24 publications

(14 citation statements)

References 31 publications

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“…The activation of signal transduction by TLRs and the expression of target gene play a key role in inflammation. A few studies suggested that n-3 PUFAs have anti-inflammatory effects [ 22 , 23 ]. TLRs combine with pathogen-related molecules non-specifically and activate NF-kB, thus leading to the expression of TNF-α, IL-1β, IL-6, IL-8 and cyclooxygenase-2 (COX-2).…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: The effect of n-3/n-6 polyunsaturated fatty acids on acute reflux esophagitis in rats

et al. 2016

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BackgroundPolyunsaturated fatty acids (PUFAs) play various roles in inflammation. However, the effect of PUFAs in the development of reflux esophagitis (RE) is unclear. This study is to investigate the potential effect of n-3/n-6 PUFAs on acute RE in rats along with the underlying protective mechanisms.MethodsForty Sprague Dawley rats were randomly divided into four groups (n = 10 in each group). RE model was established by pyloric clip and section ligation. Fish oil- and soybean oil-based fatty emulsion (n-3 and n-6 groups), or normal saline (control and sham operation groups) was injected intraperitoneally 2 h prior to surgery and 24 h postoperatively (2 mL/kg, respectively). The expressions of interleukin (IL)-1β, IL-8, IL-6 and myeloid differentiation primary response gene 88 (MyD88) in esophageal tissues were evaluated by Western blot and immunohistochemistry after 72 h. The malondialdehyde (MDA) and superoxide dismutase (SOD) expression in the esophageal tissues were determined to assess the oxidative stress.ResultsThe mildest macroscopic/microscopic esophagitis was found in the n-3 group (P < 0.05). The expression of IL-1β, IL-8, IL-6 and MyD88 were increased in all RE groups, while the lowest and highest expression were found in n-3 and n-6 group, respectively (P < 0.05). The MDA levels were increased in all groups (P < 0.05), in an ascending trend from n-3, n-6 groups to control group. The lowest and highest SOD levels were found in the control and n-3 group, respectively (P < 0.05).Conclusionn-3 PUFAs may reduce acute RE in rats, which may be due to inhibition of the MyD88-NF-kB pathway and limit oxidative damage.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: The effect of n-3/n-6 polyunsaturated fatty acids on acute reflux esophagitis in rats

et al. 2016

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“…Several animal studies have demonstrated that ischaemic myalgia is driven by IR-induced increase in muscle interleukin-1 beta (IL-1β) and characterised by local mechanical hypersensitivity, decreased muscle strength, and decreased voluntary activity [31,32]. Conversely, n-3 PUFA inhibits the increase in IL-1β after IR injury [33][34][35]. In the present study, n-3 PUFA seemingly attenuated quadriceps muscle mechanical hypersensitivity through inhibiting IL-1β, increased by tourniquet-induced IR injury.…”

Section: Discussionmentioning

confidence: 99%

Association between serum n-3 polyunsaturated fatty acids and quadriceps weakness immediately after total knee arthroplasty

Kubo¹,

Sugiyama²,

Takachu³

et al. 2020

PLoS ONE

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ObjectivesQuadriceps weakness (QW) following total knee arthroplasty (TKA) can be elicited by tourniquet-induced ischaemia reperfusion (IR), which causes a vigorous acute inflammatory response. Dietary n-3 polyunsaturated fatty acids (PUFA) are important determinants of organ and tissue protection from IR. This study aimed to examine the association between serum n-3 PUFA levels and QW, knee pain, and knee swelling immediately after TKA. MethodsA total of 32 patients who underwent unilateral TKA participated in this prospective study. On Postoperative Day 1, serum n-3 PUFA (eicosapentaenoic acid and docosahexaenoic acid) levels were measured. Preoperatively and on Postoperative Day 4, quadriceps strength, knee pain during quadriceps testing, and knee circumference were measured. QW, knee pain, and knee swelling were defined as changes in quadriceps strength, knee pain during quadriceps testing, and knee circumference, respectively, between the preoperative to the postoperative measurement. ResultsMean serum n-3 PUFA levels were 192 μg/mL (standard deviation, 58 μg/mL) on Postoperative Day 1. All measured variables changed significantly between the preoperative and the postoperative measurement time-points (P <0.01). Quadriceps strength decreased from 1.2 to 0.4 Nm/kg (QW = −65%). Knee pain during quadriceps testing increased from 1.1 to 6.0 (knee pain = 4.0). Knee circumference increased from 40 to 44 cm (knee swelling = 10%). Multivariate analysis showed that lower serum n-3 PUFA levels were independently associated with an increased QW after adjusting for the Kellgren-Lawrence grade and the PLOS ONE | https://doi.

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“…Among the lipids, Omega‐3 polyunsaturated fatty acids, especially eicosapentaenoic acid and DHA, were proved to provide a protective effect during IIR injury. They may reduce the inflammatory response caused by IIR through Toll‐like receptor 4 and nuclear factor‐κB p65 …”

Section: Discussionmentioning

confidence: 99%

“…They may reduce the inflammatory response caused by IIR through Toll-like receptor 4 and nuclear factor-κB p65. 23 Increasing evidences [24][25][26] have proven that the production of reactive oxygen intermediates, which may lead to mucosal cell injury, has massive impact on intestinal ischemia reperfusion injury. A neutrophil proteomics research 27 of intestinal ischemia and reperfusion revealed significantly down regulated transferases and up regulated oxidoreductases.…”

Section: Discussionmentioning

confidence: 99%

Dynamic metabolomic analysis of intestinal ischemia–reperfusion injury in rats

et al. 2020

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Intestinal ischemia–reperfusion injury (IIR) is a life‐threatening abdominal emergency. Compared to traditional steady‐state works, we profiled the blood of rats over 72 hr (15 time points) and examined dynamic changes in molecular pathways during IIR. Using a series of methods designed for dynamic datasets analysis (batch effects corrections, metabolomics data reduction, and different features selection), we identified 39 significant different metabolites and discovered the trends of these molecules. Four main patterns were uncovered by a longitudinal pattern recognition method. Furthermore, pathway networks were explored to uncover the possible mechanisms of IIR. We found that IIR is a complex physiological process involved in multiple pathways, such as biosynthesis of amino acids, 2‐oxocarboxylic acid metabolism, arginine‐related metabolism, and glutathione metabolism. Among which, metabolites related with phenylalanine tyrosine and tryptophan metabolism reached a peak during the early stage of reperfusion, while molecules in biosynthesis of unsaturated fatty acids metabolism declined. Our work provides a feasible scheme to understand dynamic molecule variation and will provide new explications about the effect of intestinal ischemia reperfusion from a dynamic perspective.

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Omega-3 polyunsaturated fatty acids inhibit the increase in cytokines and chemotactic factors induced in vitro by lymph fluid from an intestinal ischemia-reperfusion injury model

Cited by 24 publications

References 31 publications

RETRACTED ARTICLE: The effect of n-3/n-6 polyunsaturated fatty acids on acute reflux esophagitis in rats

RETRACTED ARTICLE: The effect of n-3/n-6 polyunsaturated fatty acids on acute reflux esophagitis in rats

Association between serum n-3 polyunsaturated fatty acids and quadriceps weakness immediately after total knee arthroplasty

Dynamic metabolomic analysis of intestinal ischemia–reperfusion injury in rats

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