Omentum patch substitute for facilitating endoscopic repair of GI perforations: an early laparoscopic pilot study with a foam matrix plug (with video)

Bonin, Eduardo A.; Bingener, Juliane; Rajan, Elizabeth; Knipschield, Mary A.; Gostout, Christopher J.

doi:10.1016/j.gie.2012.09.020

Cited by 7 publications

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, it is reported ADSCs derived from subcutaneous adipose tissue can be regenerated and used by dermatology and in plastic surgery 17,18 . However, abdominal surgeons generally use omentum (visceral adipose tissue) for the repair of gastrointestinal perforations 19,20 . The authors have also experienced that these perforative points were completely replaced with regenerated tissues by an omental patch, thus digestive surgeons often have the impression that omentum has superior self-renewal ability.…”

Section: Discussionmentioning

confidence: 99%

The Differences in the Characteristics of Insulin-producing Cells Using Human Adipose-tissue Derived Mesenchymal Stem Cells from Subcutaneous and Visceral Tissues

Wada

Ikemoto

Morine

et al. 2019

Sci Rep

View full text Add to dashboard Cite

The aim of this study was to investigate the characteristics of insulin producing cells (IPCs) differentiated from adipose-tissue derived stem cells (ADSCs) isolated from human subcutaneous and visceral adipose tissues and identify ADSCs suitable for differentiation into efficient and functional IPCs. Subcutaneous and visceral adipose tissues collected from four (4) patients who underwent digestive surgeries at The Tokushima University (000035546) were included in this study. The insulin secretion of the generated IPCs was investigated using surface markers by: fluorescence activated cell sorting (FACS) analysis; cytokine release; proliferation ability of ADSCs; in vitro (glucose-stimulated insulin secretion: (GSIS) test/in vivo (transplantation into streptozotocin-induced diabetic nude mice). The less fat-related inflammatory cytokines secretions were observed (P < 0.05), and the proliferation ability was higher in the subcutaneous ADSCs (P < 0.05). Insulin expression and GISI were higher in the subcutaneous IPCs (P < 0.01 and P < 0.05, respectively). The hyperglycaemic state of all mice that received IPCs from subcutaneous fat tissue converted into normo-glycaemia in thirty (30) days post-transplantation (4/4,100%). Transplanted IPCs were stained using anti-insulin and anti-human leukocyte antigen antibodies. The IPCs generated from the ADSCs freshly isolated from the human fat tissue had sufficient insulin secreting ability in vitro and in vivo.

show abstract