Omeprazole determination using HPLC with coulometric detection

Sluggett, Gregory W.; Stong, John D.; Adams, James; Zhao, Zheng

doi:10.1016/s0731-7085(00)00519-7

Cited by 42 publications

(19 citation statements)

References 24 publications

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“…Therefore, determining of these drugs in pharmaceutical formulations and biological samples is very important. Many analytical methods, such as highperformance liquid chromatography (HPLC) [5][6][7][8][9][10][11][12], microdialysis and HPLC [13], HPLC-mass spectrometry [14], HPLC-tandem mass spectrometry [15], HPLC with coulometric detection [16], liquid chromatography-electrospray quadrupole liner ion trap mass spectrometry [17], solid phase extraction and micellar electrokinetic capillary chromatography [18], liquid chromatography with liquidliquid extraction [19], UV-visible spectrophotometry [20][21][22], and derivative spectroscopy [23,24], have been used for quantitative determination of pantoperazole and omoprazole in drug formulations, plasma, and other biological fluids. Although the chromatographic methods have sufficient sensitivity for determination of OMZ and PNZ in pharmaceutical dosage forms and in biological fluids, they require sample pretreatment and time-consuming extraction steps prior to assay of the drugs.…”

Section: Introductionmentioning

confidence: 99%

Sensitive amperometric detection of omeprazole and pantoperazole at electrodeposited nickel oxide nanoparticles modified glassy carbon electrode

Salimi

Enferadi

Noorbakhash

et al. 2011

J Solid State Electrochem

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Glassy carbon electrode modified with electrodeposited nickel oxide nanoparticles (NiOxNPs) was used as electrocatalyst for oxidation of omeprazole and pentoperazole in alkaline solution. The modified electrode exhibited efficient electrocatalytic activity for the oxidation of omeprazole and pentoperazole with relatively high sensitivity, excellent stability, and long lifetime. Hydrodynamic amperometric method is used for determination of selected analytes. Under optimized condition, the linear concentration range, detection limit, and sensitivity of modified electrode toward omeprazole detection are 4.5-120 μM, 0.4 μM (at signal to noise 3), and 40.1 nA μM −1 cm −2 , respectively. For pantoperazole, hydrodynamic amperometric determination yielded calibration curve with linear range of 2.5-180 μM, detection limit of 0.2 μM, and sensitivity of 39.2 nA μM −1 cm −2 , respectively. The proposed method was successfully applied to pentoperazole and omeprazole determination in drug samples.

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Section: Introductionmentioning

confidence: 99%

Sensitive amperometric detection of omeprazole and pantoperazole at electrodeposited nickel oxide nanoparticles modified glassy carbon electrode

Salimi

Enferadi

Noorbakhash

et al. 2011

J Solid State Electrochem

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“…This not only allows for a more comprehensive profile of omeprazole metabolism but also, as Sluggett et al determined [18], omeprazole sulfide is an acidic degrade, and it's measurement may be helpful in evaluation sample handling.…”

Section: Discussionmentioning

confidence: 99%

A simple and sensitive bioanalytical assay for simultaneous determination of omeprazole and its three major metabolites in human blood plasma using RP-HPLC after a simple liquid–liquid extraction procedure

Rezk

Brown

Kashuba

2006

Journal of Chromatography B

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“…To date, plasma OME levels have been analyzed using high-performance liquid chromatography (HPLC) with coulometric detection 12 and ultraviolet detection (UV)…”

Section: Introductionmentioning

confidence: 99%

Improvement and Validation of a High-Performance Liquid Chromatography in Tandem Mass Spectrometry Method for Monitoring of Omeprazole in Plasma

Wojnicz

García

Román-Martínez

et al. 2015

Therapeutic Drug Monitoring

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Esta es la versión de autor del artículo publicado en: This is an author produced version of a paper published in:Therapeutic Drug Monitoring 37.3 (2015) Omeprazole (OME) is a proton pump inhibitor (PPI) with 58% bioavailability after single oral dose, presenting large inter-individual variations and significant drug-drug interactions. A simple and rapid liquid chromatography in tandem with mass spectrometry (LC/MS-MS) with solid phase extraction (SPE) and isotope-labelled internal standard (IS) method was developed to monitor the plasma levels of OME for application in pharmacokinetics and drug-drug interactions studies. OME and its IS (OME-D3), were eluted with Zorbax extend C-18 rapid resolution (4.6 mm x 50 mm, 3.5 μm) at 25ºC, under isocratic conditions through a mobile phase consisting of 1 mM ammonium acetate, pH 8.5 (55%), and acetonitrile (ACN, 45%). The flow rate was 0.8 mL/min and the run time of chromatogram was 1.2 min. OME was detected and quantified by LC-MS/MS with positive electrospray ionization (ESI) that operates in multiple-reaction monitoring (MRM) mode. The method was linear in the range of 1.5-2000 ng/mL for OME. The validation assays of accuracy and precision, matrix effect, extraction recovery and stability of the samples for OME did not deviate more than 20% for the lower limit of quantification (LLOQ) and no more than 15% for other quality controls (QCs), according to regulatory agencies. Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation AbstractOmeprazole (OME) is a proton pump inhibitor (PPI) with 58% bioavailability after a single oral dose. It is subject to marked inter-individual variations and significant drugdrug interactions. The authors developed a simple and rapid method based on liquid chromatography in tandem with mass spectrometry (LC/MS-MS) with solid phase extraction (SPE) and isotope-labelled internal standard (IS) to monitor plasma levels of OME in pharmacokinetics and drug-drug interaction studies. OME and its IS (OME-D3) were eluted with the Zorbax Extend C-18 rapid resolution column (4.6 mm x 50 mm, 3.5 μm) at 25ºC, under isocratic conditions through a mobile phase consisting of 1 mM ammonium acetate, pH 8.5 (55%), and acetonitrile (ACN, 45%). The flow rate was 0.8 mL/min, and the chromatogram run time was 1.2 min. OME was detected and quantified by LC-MS/MS with positive electrospray ionization (ESI), which operates in multiple-reaction monitoring (MRM) mode. The method was linear in the range of 1.5-2000 ng/mL for OME. The validation assays for accuracy and precision, matrix effect, extraction recovery, and stability of the samples for OME did not deviate more than 20% for the lower limit of quantification (LLOQ) and no more than 15% for other quality controls (QCs). These findings are consistent with the requirements of regulatory agencies.

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Omeprazole determination using HPLC with coulometric detection

Cited by 42 publications

References 24 publications

Sensitive amperometric detection of omeprazole and pantoperazole at electrodeposited nickel oxide nanoparticles modified glassy carbon electrode

Sensitive amperometric detection of omeprazole and pantoperazole at electrodeposited nickel oxide nanoparticles modified glassy carbon electrode

A simple and sensitive bioanalytical assay for simultaneous determination of omeprazole and its three major metabolites in human blood plasma using RP-HPLC after a simple liquid–liquid extraction procedure

Improvement and Validation of a High-Performance Liquid Chromatography in Tandem Mass Spectrometry Method for Monitoring of Omeprazole in Plasma

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