Omics data reveals the phenolic fingerprint of Brazilian whole wheat flours of different technological qualities

Santos, Millena Cristina Barros; D’Almeida, Carolina Thomaz dos Santos; Cameron, Luiz Cláudio; Gutkoski, Luiz Carlos; Ferreira, Mariana Simões Larraz

doi:10.1007/s13197-023-05665-8

Cited by 2 publications

(2 citation statements)

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“…The total phenolic content was calculated by estimating the total reducing capacity of the extracts using the Folin-Ciocalteu reagent based on the classical method 21 adapted to 96-well microplates. 19 The absorbance was read at 750 nm on a microplate reader (FlexStation III, Molecular Devices, San Jose, California). Gallic acid was used as the standard (0−250 μg/mL; R 2 = 0,999).…”

Section: Metabolite Extractionmentioning

confidence: 99%

“…In the last years, metabolomics approaches have been employed to promote a comprehensive characterization of specialized metabolites in cereal samples. ,− Most of the metabolomics-based studies focus on major crops, such as maize, rice, and wheat, while other crops, such as triticale, millet, and even sorghum, are still underexplored. According to the Web of Science platform, searching by “metabolomics” and the common name of cereal crops, the following results were obtained: “rice”, 1068 articles; “wheat”, 725; “maize” 508; “corn”, 269; “barley”, 263; “oat”, 93; “sorghum”, 85; “rye”, 75; “pearl millet”, 7, and “triticale”, 1.…”

Section: Introductionmentioning

confidence: 99%

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Overview of the Metabolite Composition and Antioxidant Capacity of Seven Major and Minor Cereal Crops and Their Milling Fractions

Ribeiro da Silva Lima,

Barros Santos,

P. Gomes

et al. 2024

J. Agric. Food Chem.

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Cereal grains play an important role in human health as a source of macro-and micronutrients, besides phytochemicals. The metabolite diversity was investigated in cereal crops and their milling fractions by untargeted metabolomics ultra-high-performance liquid chromatography−tandem mass spectrometry (UHPLC−MS/MS) of 69 samples: 7 species (barley, oat, pearl millet, rye, sorghum, triticale, and wheat), 23 genotypes, and 4 milling fractions (husk, bran, flour, and wholegrain). Samples were also analyzed by in vitro antioxidant activity. UHPLC−MS/MS signals were processed using XCMS, and metabolite annotation was based on SIRIUS and GNPS libraries. Bran and husk showed the highest antioxidant capacity and phenolic content/ diversity. The major metabolite classes were phenolic acids, flavonoids, fatty acyls, and organic acids. Sorghum, millet, barley, and oats showed distinct metabolite profiles, especially related to the bran fraction. Molecular networking and chemometrics provided a comprehensive insight into the metabolic profiling of cereal crops, unveiling the potential of coproducts and super cereals such as sorghum and millet as sources of polyphenols.

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Section: Metabolite Extractionmentioning

confidence: 99%