Omics of antimicrobials and antimicrobial resistance

Чернов, В. М.; Чернова, О. А.; Mouzykantov, Alexey A.; Lopukhov, L. V.; Aminov, Rustam

doi:10.1080/17460441.2019.1588880

Cited by 63 publications

(49 citation statements)

References 148 publications

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“…The emergence of drug resistance among pathogens has assumed alarming proportions in recent times, causing rapid depletion in the current armamentarium of drugs to fight such infections [1]. This has posed a serious threat to human health globally and required the rapid development of new and effective drugs at a pace faster than resistance to achieve desirable outcomes in the treatment of infectious diseases.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a New Endophytic Actinobacterium Streptomyces californicus Strain ADR1 as a Promising Source of Anti-Bacterial, Anti-Biofilm and Antioxidant Metabolites

Singh

Dubey

2020

Microorganisms

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In view of the fast depleting armamentarium of drugs against significant pathogens, like methicillin-resistant Staphylococcus aureus (MRSA) and others due to rapidly emerging drug-resistance, the discovery and development of new drugs need urgent action. In this endeavor, a new strain of endophytic actinobacterium was isolated from the plant Datura metel, which produced secondary metabolites with potent anti-infective activities. The isolate was identified as Streptomyces californicus strain ADR1 based on 16S rRNA gene sequence analysis. Metabolites produced by the isolate had been investigated for their antibacterial attributes against important pathogens: S. aureus, MRSA, S. epidermis, Enterococcus faecium and E. faecalis. Minimum inhibitory concentration (MIC90) values against these pathogens varied from 0.23 ± 0.01 to 5.68 ± 0.20 μg/mL. The metabolites inhibited biofilm formation by the strains of S. aureus and MRSA (Biofilm inhibitory concentration [BIC90] values: 0.74 ± 0.08–4.92 ± 0.49 μg/mL). The BIC90 values increased in the case of pre-formed biofilms. Additionally, the metabolites possessed good antioxidant properties, with an inhibitory concentration (IC90) value of 217.24 ± 6.77 µg/mL for 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging. An insight into different classes of compounds produced by the strain ADR1 was obtained by chemical profiling and GC-MS analysis, wherein several therapeutic classes, for example, alkaloids, phenolics, terpenes, terpenoids and glycosides, were discovered.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a New Endophytic Actinobacterium Streptomyces californicus Strain ADR1 as a Promising Source of Anti-Bacterial, Anti-Biofilm and Antioxidant Metabolites

Singh

Dubey

2020

Microorganisms

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“…The analysis of modifications in the pattern of protein expression in response to antimicrobial agents and the classification of the differentially expressed microbial proteins leads to disclosure of specific responses that may occur after drug administration constructing the molecular networks and pathways. Detection of post-translational modifications associated with antimicrobial resistance supports the identification of potential targets for preventing the development of resistance [ 231 ]. Recent studies have shown that protein post-translational modifications can play a role in bacterial antibiotic resistance.…”

Section: Proteomics Applications In Antibiotic Resistancementioning

confidence: 99%

Applied Proteomics in ‘One Health’

Katsarou

Billinis

Galamatis³

et al. 2021

Proteomes

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‘One Health’ summarises the idea that human health and animal health are interdependent and bound to the health of ecosystems. The purpose of proteomics methodologies and studies is to determine proteins present in samples of interest and to quantify changes in protein expression during pathological conditions. The objectives of this paper are to review the application of proteomics technologies within the One Health concept and to appraise their role in the elucidation of diseases and situations relevant to One Health. The paper develops in three sections. Proteomics Applications in Zoonotic Infections part discusses proteomics applications in zoonotic infections and explores the use of proteomics for studying pathogenetic pathways, transmission dynamics, diagnostic biomarkers and novel vaccines in prion, viral, bacterial, protozoan and metazoan zoonotic infections. Proteomics Applications in Antibiotic Resistance part discusses proteomics applications in mechanisms of resistance development and discovery of novel treatments for antibiotic resistance. Proteomics Applications in Food Safety part discusses the detection of allergens, exposure of adulteration, identification of pathogens and toxins, study of product traits and characterisation of proteins in food safety. Sensitive analysis of proteins, including low-abundant ones in complex biological samples, will be achieved in the future, thus enabling implementation of targeted proteomics in clinical settings, shedding light on biomarker research and promoting the One Health concept.

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“…As is evident from Figure 9b plot of F0/F versus concentration of sm-DNA shows a linear relationship. The slope of this curve 7.73×10 6 gives Ksv. On average the fluorescence lifetime for a macromolecule is approximately 10 -8 seconds.…”

Section: Fluorescence Spectroscopymentioning

confidence: 99%

“…Imidazole is a versatile ring with paramount biological importance [5][6][7] which is evident from the fact that it constitutes skeleton of many commercial drugs ( Figure 1) viz. metronidazole (antimicrobial), antifungal imidazole drugs, cimetidine (Histamine H2-receptor antagonist) and flumazenil (GABAA receptor antagonist).…”

Section: Introductionmentioning

confidence: 99%

Rationale design and synthesis of some novel imidazole linked thiazolidinone hybrid molecules as DNA minor groove binders

War

Srivastava

2020

Eur J Chem

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A new series of imidazole linked thiazolidinone hybrid molecules was designed and subsequently synthesized through a feasible, three step reaction protocol. The structures of these molecules were established using FT-IR, 1H NMR, 13C NMR and HRMS techniques. In vitro susceptibility tests against some Gram positive (Staphylococcus aureus and Bacillus subtilis) and Gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa) exhibited broad spectrum potency of the molecules. The most potent molecule (S2A7) amongst the screened molecules, showed minimum inhibitory concentration (MIC) value not less than 2.0 µg/mL which was at par with the reference drug Streptomycin. Structure activity relationships revealed nitro and chloro groups being crucial for bioactivity when present at meta position of arylidene ring in 3-(3-(imidazol-1-yl)propyl)-5-(benzylidene)-2-(phenylimino)thiazolidin-4-one. Deoxyribonucleic acid (DNA)and bovine serum albumin (BSA) binding studies for S2A7 under simulated physiological pH were probed using UV-Visible, fluorescence quenching, gel electrophoresis and molecular docking techniques. These studies established that S2A7 has strong binding affinity towards DNA and binds at the minor groove of DNA with binding constant (Kb) of 0.1287×102 L/mol. Molecular docking simulations of S2A7 with DNA and BSA predicted binding affinity of -9.2 and -7.2 kcal/mol, respectively. Van der Waals forces and hydrogen bonding interactions were predicted as the main forces of interaction. With DNA, S2A7 exhibited specific binding affinity towards adenine-thiamine base pairs. The compound S2A7 forms a stable complex with BSA by binding at subdomain IIIA implying high bio-distribution of the compound.

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Omics of antimicrobials and antimicrobial resistance

Cited by 63 publications

References 148 publications

Isolation and Characterization of a New Endophytic Actinobacterium Streptomyces californicus Strain ADR1 as a Promising Source of Anti-Bacterial, Anti-Biofilm and Antioxidant Metabolites

Isolation and Characterization of a New Endophytic Actinobacterium Streptomyces californicus Strain ADR1 as a Promising Source of Anti-Bacterial, Anti-Biofilm and Antioxidant Metabolites

Applied Proteomics in ‘One Health’

Rationale design and synthesis of some novel imidazole linked thiazolidinone hybrid molecules as DNA minor groove binders

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