Omics‐prediction of bioactive peptides from the edible cyanobacterium Arthrospira platensis proteome

Abstract

BACKGROUND Bioinformatics approaches are widely used to evaluate the prospects of novel protein sources in bioactive peptide research. Edible cyanobacteria are considered as potential protein precursors. However, the abundance of unicellular cyanobacterial proteins is largely unknown and highly dynamic according to the cultivation conditions, which need to be considered in this research field. The objective of this work was to evaluate the protein abundance of Arthrospira platensis, as well as to map the bioactive peptide sequences from the high‐abundance proteins of the A. platensis proteome. RESULTS The high‐abundance proteins of the A. platensis proteome were identified with a high‐performance liquid chromatography–tandem mass spectrometry‐based method. A total of 593 proteins were detected and quantified. The occurrence frequency of the bioactive peptides in A. platensis proteome was calculated according to the amino acid sequences via the bioinformatics approaches. Further in silico digested by trypsin, pepsin and chymotrypsin, these proteins liberated 78, 99, and 96 bioactive peptides, respectively. In each case, angiotensin‐converting enzyme inhibitors and dipeptidyl peptidase IV inhibitors were enriched. CONCLUSION This work will help rationally design the protocols for cyanobacterial cultivation, protein pre‐treatment and peptide separation, and further produce more peptides with specific functions. © 2017 Society of Chemical Industry

Conventional and in silico approaches to select promising food-derived bioactive peptides: A review

“…Therefore, the use of these in silico tools avoids the expense of laboratory time and reagent money. Additionally, they have allowed to find new BP from alternative sources, e.g., kefir milk ( Amorim et al, 2019a ), collagen ( Nuñez, Guzmán, Valencia, Almonacid & Cárdenas, 2020 ), fish skin ( Elaziz, Hemdan, Hassanien, Oliva & Xiong, 2017 ), invasive sea grass Halophila stipulacea ( Kandemir-Cavas, Pérez-Sánchez, Mert-Ozupek & Cavas, 2019 ), Chlorella sorokiniana ( Tejano, Peralta, Yap, Panjaitan & Chang, 2019 ), cyanobacterium Arthrospira platensis ( Ji et al, 2018 ), among many others.…”

In Silico Analysis of Bioactive Peptides in Invasive Sea Grass Halophila stipulacea

“…According to the BP database called BIOPEP [12], there are more than 3500 different BPs. The sources of natural BPs can be land-, marine- or food-derived, and include seaweeds [13,14] tropical amphibians [15], cyanobacterium [16], fermented soybean meal [17], sea cucumber [18], cereal crops [19] and milk [20]. BPs are of great importance because of their positive impact on human health.…”

“…Other production processes include separation and purification techniques such as gel filtration, ultrafiltration [25,26], reverse phase ultra-flow liquid chromatography (RP-UFLC) [25], and reverse phase high performance liquid chromatography (RP-HPLC) and characterization methods such as ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) [27]. Since these production, isolation, purification and characterization protocols are time- and solvent-consuming, bioinformatics tools are increasingly used [13,16]. The role of database-aided bioinformatics tools is to quantitatively predict the structure–activity relationship.…”

Effects of Spirulina platensis on insulin secretion, dipeptidyl peptidase IV activity and both carbohydrate digestion and absorption indicate potential as an adjunctive therapy for diabetes

“…and peptides that have health benefits and reduce the risk of disease (13) . In this way, many aquatic species have been studied with the generation of new and useful bioactive peptide sources.…”

“…Edible microalgal and cyanobacterial proteins have been established as suitable precursors for the production of biologically active peptides (14) . Thus, a series of peptides isolated from microalgal or cyanobacterial hydrolysates have been demonstrated to exhibit desirable bioactivities, such as antioxidative, anticancer, anti-inflammatory and antihypertensive properties (13,(16)(17)(18) .…”