Omp2b Porin Alteration in the Course of Evolution of Brucella spp.

Cloeckaert, Axel; Vergnaud, Gilles; Zygmunt, Michel S.

doi:10.3389/fmicb.2020.00284

Cited by 3 publications

(3 citation statements)

References 46 publications

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“…Brucella is a Gram-negative, facultative intracellular parasitic bacteria. Brucella can infect humans and a variety of animals, causing brucellosis ( Cloeckaert et al, 2020 ). Different Brucella species have unique host preferences, virulence, and pathogenicity ( Rajendhran, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Nonclassical intracellular Brucella , more distantly related phylogenetically to classic species, includes isolates from red foxes, Australian rodents, several frog species, rays, and human cases. These nonclassical intracellular Brucella represent several potential new species, with B. inopinata isolated from human cases being the sole representative ( Cloeckaert et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Comparative genomic analysis provides insights into the genetic diversity and pathogenicity of the genus Brucella

Yang,

Chai,

Wang

et al. 2024

Front. Microbiol.

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Some Brucella spp. are important pathogens. According to the latest prokaryotic taxonomy, the Brucella genus consists of facultative intracellular parasitic Brucella species and extracellular opportunistic or environmental Brucella species. Intracellular Brucella species include classical and nonclassical types, with different species generally exhibiting host preferences. Some classical intracellular Brucella species can cause zoonotic brucellosis, including B. melitensis, B. abortus, B. suis, and B. canis. Extracellular Brucella species comprise opportunistic or environmental species which belonged formerly to the genus Ochrobactrum and thus nowadays renamed as for example Brucella intermedia or Brucella anthropi, which are the most frequent opportunistic human pathogens within the recently expanded genus Brucella. The cause of the diverse phenotypic characteristics of different Brucella species is still unclear. To further investigate the genetic evolutionary characteristics of the Brucella genus and elucidate the relationship between its genomic composition and prediction of phenotypic traits, we collected the genomic data of Brucella from the NCBI Genome database and conducted a comparative genomics study. We found that classical and nonclassical intracellular Brucella species and extracellular Brucella species exhibited differences in phylogenetic relationships, horizontal gene transfer and distribution patterns of mobile genetic elements, virulence factor genes, and antibiotic resistance genes, showing the close relationship between the genetic variations and prediction of phenotypic traits of different Brucella species. Furthermore, we found significant differences in horizontal gene transfer and the distribution patterns of mobile genetic elements, virulence factor genes, and antibiotic resistance genes between the two chromosomes of Brucella, indicating that the two chromosomes had distinct dynamics and plasticity and played different roles in the survival and evolution of Brucella. These findings provide new directions for exploring the genetic evolutionary characteristics of the Brucella genus and could offer new clues to elucidate the factors influencing the phenotypic diversity of the Brucella genus.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative genomic analysis provides insights into the genetic diversity and pathogenicity of the genus Brucella

Yang,

Chai,

Wang

et al. 2024

Front. Microbiol.

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“…Brucellosis is a zoonotic disease caused by Brucella species. The number of species in this genus has been gradually expanded by the discovery of strains from wildlife animal species, such as amphibians and fish (1). Four major pathogenic Brucella species causing disease in humans are Brucella abortus (cattle, buffalo), Brucella melitensis (goats, sheep, camels), Brucella suis (pigs), and Brucella canis (dogs) (2).…”

Section: Introductionmentioning

confidence: 99%

A specific reverse complement sequence for distinguishing Brucella canis from other Brucella species

Yang²,

Dong-Liang³

et al. 2022

Front. Vet. Sci.

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Canine brucellosis is primarily caused by Brucella canis, but other Brucella species can also cause the disease. Identifying sequences specific to B. canis and establishing PCR assays that can distinguish between B. canis and other Brucella species is essential to determine the etiology of canine brucellosis and the source of infection and to achieve effective control. We analyzed the gaps and SNPs of genomes I and II from B. canis strain RM6/66 and B. melitensis strain 16M using the Mauve genome alignment software, and the specificity of each of these differential regions was analyzed by BLAST. A 132 bp specific sequence was found between the DK60_915 (glycosyl hydrolase 108 family protein) and DK60_917 (aldose 1-epimerase) loci in B. canis chromosome 1. Further comparative analysis revealed that this is a reverse complement sequence between B. canis and other Brucella species. Then, three primers were designed based on the sequence that could detect B. canis with a 310 bp amplification product or other Brucella species with a 413 bp product. The PCR based on these primers had reasonable specificity and a sensitivity of 100 copies of Brucella DNA. The detection results for the blood samples of the aborted dogs showed a favorable accordance with the Bruce-ladder multiplex PCR assay. In conclusion, we found a specific reverse complement sequence between B. canis and other Brucella and developed a PCR method that allows a more comprehensive identification of the pathogen involved in canine brucellosis. These findings provide an effective means for preventing and controlling brucellosis.

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Prevalence of Brucella Infections among Students of Amran University, Yemen

Al-Hadheq,

Al-Mahbashi,

Al-Wajeeh

et al. 2024

Preprint

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Background and Aim: Brucella infection in animals is considered a significant problem in most countries around the world and is regarded as one of the important pathogenic zoonotic infections affecting humans. This study aimed to determine the prevalence of Brucella infections among students at Amran University, Yemen. Methods: This is a cross-sectional study carried out among 247 students between January 2023 and November 2023. Blood samples were collected and analyzed using immunochromatographic technology. Results: Out of 247 human samples, 2 (1.75%) were positive among males, and 15 (12.5%) were positive among females. Additionally, 49 (19.8%) of the positive cases were from Amran city, while 198 (80.2%) were from rural areas, with significant differences (P < 0.05). The risk factors for Brucella infection included ranching, consuming unpasteurized milk, and direct contact with livestock. Conclusion: The prevalence of brucellosis was low among the study participants; however, it could become a serious problem that threatens the healthcare system in Yemen.

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Omp2b Porin Alteration in the Course of Evolution of Brucella spp.

Cited by 3 publications

References 46 publications

Comparative genomic analysis provides insights into the genetic diversity and pathogenicity of the genus Brucella

Comparative genomic analysis provides insights into the genetic diversity and pathogenicity of the genus Brucella

A specific reverse complement sequence for distinguishing Brucella canis from other Brucella species

Prevalence of Brucella Infections among Students of Amran University, Yemen

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