OmpA of Klebsiella pneumoniae ATCC 13883 induces pyroptosis in HEp-2 cells, leading to cell-cycle arrest and apoptosis

You, Hee Sang; Lee, Song Hee; Kang, Sang Sun; Hyun, Sung Hee

doi:10.1016/j.micinf.2020.06.002

Cited by 7 publications

(7 citation statements)

References 41 publications

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“…In addition to the attenuated lung injury, in this thesis, our study explored the problem that PSPAs regulated the innate immune system through pyroptosis. Pyroptosis, different from apoptosis, autophagy, and anoikis, is a pro-inflammatory death mode (membrane permeabilizing) with necrotic morphological characteristics, which is the innate immune response to the pathogen in infected host cells, and it is an important regulated cell death in KP-infected macrophage [ 12 , 46 ]. However, KP, as a facultative intracellular pathogen, induces necrosis (membrane permeabilizing) in bacteria-burdened cells to promote the release of bacteria, infect new cells, and drive further immune cell recruitment and tissue damage.…”

Section: Discussionmentioning

confidence: 99%

“…Pyroptosis is the innate immune response to the pathogen in infected host cells. Accumulating evidence indicates that pyroptosis in alveolar macrophages (AMs) is related to NLRP3 inflammasome, which is activated by invading of KP [ 11 ], and then induces the releasing of the secondary pro-inflammatory factors to participate in the elimination of invasive bacteria [ 12 ]. However, investigators found that pyroptosis induced by lethal toxin can lead to significant lung tissue injury [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in Klebsiella pneumoniae Infection

Dong

Wang

et al. 2021

IJMS

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Given the rise of morbidity and mortality caused by Klebsiella pneumoniae (KP), the increasing number of strains resistant to antibiotics, and the emergence of hypervirulent Klebsiella pneumonia, treatment of KP infection becomes difficult; thus, novel drugs are necessary for treatment. Anthocyanins, or natural flavonoids, have an extensive effect against bacterial infection. However, few studies on anti-KP are identified. Here, we evaluated the therapeutic effect of purple sweet potato anthocyanins (PSPAs) on KP, containing 98.7% delphinidin 3-sambubioside. Results showed that KP-infected mice after PSPAs treatment manifested decreased mortality, weakened lung injury, dampened inflammatory responses, and reduced bacterial systemic dissemination in vivo. In Vitro, PSPAs significantly suppressed pyroptosis and restricted NLRP3 inflammasome activation in alveolar macrophages infected with KP. As for the mechanism, PSPAs promote mitophagy by recruiting Parkin to the mitochondria. PSPAs-conferred mitophagy increased mitochondrial membrane potential and decreased mitochondrial reactive oxygen species and mitochondrial DNA, resulting in impaired NLRP3 inflammasome activation. In addition, the promotion of mitophagy by PSPAs required the Nrf2 signaling pathway. Collectively, these findings suggest that PSPAs are a potential option for the treatment of KP infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in Klebsiella pneumoniae Infection

Dong

Wang

et al. 2021

IJMS

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“…When HEp-2 cells were treated with OmpA, the transmembrane porin protein of Kp (ATCC 13883), OmpA induced cell cycle arrest, apoptosis, and pyroptosis. These events may act as host defense mechanisms against Kp infection ( 40 ). In addition, caspase-11-mediated IL-1α and IL-1β secretion, and pyroptosis were involved in the early stages of Kp infection ( 41 ).…”

Section: Discussionmentioning

confidence: 99%

Resistance of hypervirulent Klebsiella pneumoniae to cathepsin B-mediated pyroptosis in murine macrophages

et al. 2023

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IntroductionHypervirulent Klebsiella pneumoniae (hvKp) has emerged as a clinically significant global pathogen in the last decade. However, the host immune responses of the macrophages during hvKp infection are largely unknown. In the present study, we aimed to compare the cytotoxic effects of hvKp and classical K. pneumoniae (cKp) in murine macrophages.ResultsWe found that the activation of caspase-1 -dependent pyroptosis was higher in cKp-infected macrophages compared with that in hvKp-infected macrophages. In Caspase-1 deficiency macrophages, pyroptosis diminished during infection. Both hvKp and cKp strains led to nucleotide-binding and oligomerization domain-like receptor protein 3 (NLRP3) inflammasome formation and lysosomal cathepsin B activation, thus resulting in pyroptosis. Compared with the cKp strain, the hvKp strain inhibited these phenomena in murine macrophages.ConclusionHvKp infection resulted in different levels of pyroptosis via the activation of cathepsin B-NLRP3-caspase-1 in murine macrophages. Therefore, the manipulation of pyroptotic cell death is a potential target for host response during hvKp infection in macrophages.

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“…Outer membrane proteins (OMPs) are implicated in the modulation of immune response and antibiotic resistance as an essential factor. In Kpn infection, OmpA activates Toll-like receptor (TLR) 2, and OmpK36 contributes to resisting phagocytosis by neutrophils and macrophages [19][20][21]. On the other hand, OmpK35 and OmpK26 are associated with the efflux of antibiotics such as carbapenem [22].…”

Section: Kpn Virulence Factorsmentioning

confidence: 99%

Plant-Origin Components: New Players to Combat Antibiotic Resistance in Klebsiella pneumoniae

Luna-Pineda,

Rodríguez-Martínez,

Salazar-García

et al. 2024

IJMS

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Klebsiella pneumoniae (Kpn) is an opportunistic pathogen that causes intrahospital complications such as pneumonia, liver abscesses, soft tissue infections, urinary infections, bacteraemia, and, in some cases, death. Since this bacterium has a higher frequency than other Gram-negative pathogens, it has become an important pathogen to the health sector. The adaptative genome of Kpn likely facilitates increased survival of the pathogen in diverse situations. Therefore, several studies have been focused on developing new molecules, synergistic formulations, and biomaterials that make it possible to combat and control infections with and dispersion of this pathogen. Note that the uncontrolled antibiotic administration that occurred during the pandemic led to the emergence of new multidrug-resistant strains, and scientists were challenged to overcome them. This review aims to compile the latest information on Kpn that generates intrahospital infections, specifically their pathogenicity-associated factors. Furthermore, it explains the natural-product-based treatments (extracts and essential oils) developed for Kpn infection and dispersion control.

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OmpA of Klebsiella pneumoniae ATCC 13883 induces pyroptosis in HEp-2 cells, leading to cell-cycle arrest and apoptosis

Cited by 7 publications

References 41 publications

Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in Klebsiella pneumoniae Infection

Anthocyanin Extract from Purple Sweet Potato Exacerbate Mitophagy to Ameliorate Pyroptosis in Klebsiella pneumoniae Infection

Resistance of hypervirulent Klebsiella pneumoniae to cathepsin B-mediated pyroptosis in murine macrophages

Plant-Origin Components: New Players to Combat Antibiotic Resistance in Klebsiella pneumoniae

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