On and off responses of the photopic fullfield ERG in normal subjects and glaucoma patients

Horn, Folkert K.; Gottschalk, K.; Mardin, Christian Y.; Pangeni, Gobinda; Jünemann, Anselm; Kremers, Jan

doi:10.1007/s10633-011-9258-1

Cited by 29 publications

(41 citation statements)

References 27 publications

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“…[45][46][47] The broadening of the d-wave peak in the focal ERG and the presence of the 3PP in the full-field long ERG in participants with ADOA may be due to contributions from the cone receptor potential and/or depolarizing OFF-bipolar cell responses after light offset, which were unmasked in the relative absence of the negative going PhNR OFF . [48][49][50] The 2PP may be the i OFF -wave described by Horn et al, 51 although in contrast to their results, this study did not record a significant difference in amplitude between controls and participants with ADOA.…”

Section: Comparison Of Focal and Full-field Phnrscontrasting

confidence: 93%

Electrophysiological ON and OFF Responses in Autosomal Dominant Optic Atrophy

Morny

Margrain

Binns

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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METHODS. Twelve participants from six families with OPA1 ADOA and 16 age-matched controls were recruited. Electrophysiological assessment involved pattern ERGs (PERGs), focal (208) and full-field long-duration (250 ms) flash ERGs using a red light-emitting diode flash on a rod-saturating blue background, and full-field brief (300 ls) xenon flash ERGs using a red filter over a continuous rod saturating blue background. Amplitudes and implicit times of the ERG components were analyzed and the diagnostic potential of each electrophysiological technique was determined by generating receiver operating characteristic (ROC) curves.RESULTS. Mean amplitudes of the N95 and all PhNRs, except the full-field PhNR ON , were significantly reduced in participants with ADOA (P < 0.01). Subtraction of the group-averaged focal ERG of ADOA participants from that of controls showed an equal loss in the focal PhNR ON and PhNR OFF components, whereas in the full-field ERG the loss in the PhNR OFF was greater than that in the PhNR ON component. The areas under the ROC curve (AUC) for the focal PhNR ON (0.92), focal PhNR OFF (0.95), and full-field PhNR OFF (0.83), were not significantly different from that of the PERG N95 (0.99).CONCLUSIONS. In patients with ADOA, the PhNR ON and PhNR OFF components are nearly symmetrically reduced in the long-duration ERG, suggesting that ON-and OFF-RGC pathways may be equally affected.

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Section: Comparison Of Focal and Full-field Phnrscontrasting

confidence: 93%

Electrophysiological ON and OFF Responses in Autosomal Dominant Optic Atrophy

Morny

Margrain

Binns

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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“…There are less (and somewhat inconsistent) studies so far examining PhNR in ocular hypertension: North et al 29 and Viswanathan et al 18 found reduced PhNR amplitudes in patients with OHT. Colotto et al 50 and Horn et al 51 in Above the spectra, the response frequency in Hz with P value and amplitude in lV are given. P values were calculated based on the signal-to-noise-ratio.…”

Section: Comparison Of Phnr and Perg In Other Studiesmentioning

confidence: 99%

“…Different stimulus conditions might be one reason for these discrepancies: North 29 and Viswanathan 18 used short flashes (5 ms), Horn 51 and Colotto 50 used long flashes (200 ms). A second reason might be small sample sizes.…”

Section: Comparison Of Phnr and Perg In Other Studiesmentioning

confidence: 99%

Photopic Negative Response versus Pattern Electroretinogram in Early Glaucoma

Preiser¹,

Lagrèze

Bach³

et al. 2013

Invest. Ophthalmol. Vis. Sci.

110

103

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PURPOSE. Photopic negative response (PhNR) and pattern electroretinogram (PERG) are electrophysiological markers of retinal ganglion cell function; both are reduced in glaucoma. We compared PhNR and PERG in different stages of the disease.METHODS. Eleven eyes with preperimetric glaucoma (glaucomatous optic disc with normal field); 18 with manifest glaucoma; and 26 normals were included. We obtained PhNR (flash strength from 0.1-4 cdÁs/m 2 ) and steady-state PERG and analyzed PhNR amplitude (baseline to 72 ms trough); PhNR/ b-wave ratio; PERG amplitude; and PERG ratio (0.88/168).RESULTS. Identification of PhNR structure was only reliable ‡1 cdÁs/m 2 flash strength; amplitude and receiver operating characteristics (ROC) area under curve (AUC) changed little from 1 to 4 cdÁs/m 2 . Both PhNR and PERG (amplitude and ratio) were reduced in preperimetric and more so in manifest glaucoma. AUCs based on PhNR/PERG amplitudes were not significantly different from chance in preperimetric glaucoma (AUCs 0.61/0.59), but were significant in manifest glaucoma (0.78/0.76); ratios were significant in both glaucoma groups (0.80/0.73 and 0.80/0.79). In spite of that, PhNR ratio and PERG ratio were not significantly correlated (r ¼ 0.22 across all groups); an ROC based on a combination of both reached AUCs of 0.85/0.90 for preperimetric/manifest glaucoma.CONCLUSIONS. Both PhNR and PERG performed similarly to detect glaucoma; for both, ratios performed better than amplitudes. The PhNR has the advantage of not requiring clear optics and refractive correction; the PERG has the advantage of being recorded with natural pupils. (Invest Ophthalmol Vis Sci.

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“…It can be advantageous to study the on-and off-responses separately. To separate these responses, long-duration flashes have been used [11][12][13][14][15][16]. Owing to the flash duration in the long-flash ERG, fast adaptation mechanisms may cause some adaptation to the different light levels.…”

Section: Introductionmentioning

confidence: 99%

“…In clinical studies, the PhNR of the flash ERG has been reported to be an indicator of glaucomatous damage of the retinal ganglion cells [5,16,35].…”

Section: Introductionmentioning

confidence: 99%

On- and off-response ERGs elicited by sawtooth stimuli in normal subjects and glaucoma patients

et al. 2012

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The aim of this study is to measure the on- and off-responses and their response asymmetries elicited by sawtooth stimuli in normal subjects and glaucoma patients. Furthermore, the correlation between the ERGs and other functional and structural parameters are investigated. Full-field stimuli were produced using a Ganzfeld bowl with Light Emitting Diodes (LEDs) as light sources. On- and off-response ERGs were recorded from 17 healthy subjects, 12 pre-perimetric and 15 perimetric glaucoma patients using 4-Hz luminance rapid-on and rapid-off sawtooth stimuli (white light; mean luminance 55 cd/m(2)) at 100% contrast. The on- and off-responses were added to study response asymmetries. In addition, flash ERGs were elicited by red stimuli (200 cd/m(2)) on a blue background (10 cd/m(2)). The mean deviations (MD) of the visual field defects were obtained by standard automated perimetry. The retinal nerve fibre layer thickness (RNFLT) was measured with Spectral Domain Optical Coherence Tomography (SOCT). We studied the correlation between ERG response amplitudes, visual field mean deviation (MDs) and RNFLT values. The on-responses showed an initial negative (N-on) followed by a positive (P-on), a late positive (LP-on) and a late negative responses (LN-on). The off-responses showed an initial positive (P-off) a late positive (LP-off) and a late negative response (LN-off). The addition of on- and off-responses revealed an initial positive (P-add) and a late negative response (LN-add). The on-response components (N-on, P-on and LN-on) in the glaucoma patients were relatively similar to those of the control subjects. However, the LP-on was significantly elevated (p = 0.03) in perimetric patients. The LP-off was significantly elevated (p < 0.001), and the amplitude of LN-off was significantly reduced in perimetric patients (p = 0.02). The LN-add amplitude was significantly reduced (p < 0.001) and delayed (p = 0.03) in perimetric patients. The amplitudes of the LN-off and LN-add ERG components were significantly correlated with the PhNR in the flash ERG (LN-off: p = 0.01; LN-add: p < 0.001) and with RNFLT (LN-off: p = 0.006; LN-add: p = 0.001). On- and off-response ERGs and their response asymmetries, elicited by sawtooth stimuli, are altered in the glaucoma patients. The late components are affected. Changes in the late negative components are correlated with structural and other functional changes.

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On and off responses of the photopic fullfield ERG in normal subjects and glaucoma patients

Cited by 29 publications

References 27 publications

Electrophysiological ON and OFF Responses in Autosomal Dominant Optic Atrophy

Electrophysiological ON and OFF Responses in Autosomal Dominant Optic Atrophy

Photopic Negative Response versus Pattern Electroretinogram in Early Glaucoma

On- and off-response ERGs elicited by sawtooth stimuli in normal subjects and glaucoma patients

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