On-chip analysis of glycolysis and mitochondrial respiration in human induced pluripotent stem cells

Human In Vitro Models of Neuroenergetics and Neurometabolic Disturbances: Current Advances and Clinical Perspectives

“…Recent research efforts are aiming to either modifying complex models to suit the Seahorse XF analyzer (eg, re-plating of organoids 55 ) or transferring the principle of measuring OCR and ECAR to customized OoC platforms. 56 …”

Microphysiological pancreas-on-chip platform with integrated sensors to model endocrine function and metabolism

“…[35][36][37] Recent studies demonstrated that oxygen sensors integrated in microfluidic systems can be a promising read-out tool to assess cell functionality. 8,[38][39][40][41] Here, we introduce a novel pancreas-on-chip platform based on a tailored thermoplastic-based microfluidic system that addresses the key requirements on an in vitro model of the endocrine pancreas. The developed platform leverages a new and robust strategy based on hydrodynamic trapping for self-guided loading of spheroids, e.g.…”

“…35–37 Recent studies demonstrated that oxygen sensors integrated in microfluidic systems can be a promising read-out tool to assess cell functionality. 8,38–41…”

Design of dual peptide-conjugated hydrogels for proliferation and differentiation of human pluripotent stem cells

“…Several clinical trials have incorporated differentiated cells from hPSCs (human pluripotent stem cells), hiPSCs (human induced pluripotent stem cells) or hESCs (human embryonic stem cells) because any types of tissue cells in the human body can be derived from hPSCs with appropriate differentiation protocols [ [1] , [2] , [3] , [4] , [5] , [6] , [7] ]. For the maintenance and expansion of clinical-grade hPSCs, the cells should be cultured under xeno-free conditions.…”