On-chip bio-analyte detection utilizing the velocity of magnetic microparticles in a fluid

Giouroudi, Ioanna; Driesche, Sander van den; Kosel, Jürgen; Größinger, R.; Vellekoop, Michael J.

doi:10.1063/1.3556952

Cited by 11 publications

(12 citation statements)

References 14 publications

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“…Therefore, in this research, the emphasis is on the capacity of the MMC to isolate and perform selective treatments on cells tagged with SPBs on a simple and integrated platform. The approach we have taken in this paper differs from our previous work [26][27][28][29][30][31][32][33][34] and from other work in terms of simplicity of fabrication, operation of the device as well as using living cells and also by the integration of all components into a microfluidic chip. Moreover, compared to the commercially available products, the MMC is an integrated chip of small size (3.1 Â 2.3 cm 2 chip area) that enables both cell separation and experiments on-chip.…”

mentioning

confidence: 44%

“…F m , calculated for an SPB with a radius of 1.4 lm and a susceptibility of 1.2 46 in a solution with a viscosity of 1 Â 10 À3 PaÁs, has a maximum value of 2.2 pN at the edges of the disk, which is sufficient to trap SPBs. 8,9,13,15,[20][21][22][26][27][28][29][30][31][32][33][34][35]47 Fig . 2(c) shows the velocity of an SPB during the trapping process.…”

Section: -4mentioning

confidence: 99%

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Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

et al. 2014

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This study describes the development and testing of a magnetic microfluidic chip (MMC) for trapping and isolating cells tagged with superparamagnetic beads (SPBs) in a microfluidic environment for selective treatment and analysis. The trapping and isolation are done in two separate steps; first, the trapping of the tagged cells in a main channel is achieved by soft ferromagnetic disks and second, the transportation of the cells into side chambers for isolation is executed by tapered conductive paths made of Gold (Au). Numerical simulations were performed to analyze the magnetic flux and force distributions of the disks and conducting paths, for trapping and transporting SPBs. The MMC was fabricated using standard microfabrication processes. Experiments were performed with E. coli (K12 strand) tagged with 2.8 μm SPBs. The results showed that E. coli can be separated from a sample solution by trapping them at the disk sites, and then isolated into chambers by transporting them along the tapered conducting paths. Once the E. coli was trapped inside the side chambers, two selective treatments were performed. In one chamber, a solution with minimal nutrition content was added and, in another chamber, a solution with essential nutrition was added. The results showed that the growth of bacteria cultured in the second chamber containing nutrient was significantly higher, demonstrating that the E. coli was not affected by the magnetically driven transportation and the feasibility of performing different treatments on selectively isolated cells on a single microfluidic platform.

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confidence: 44%

Section: -4mentioning

confidence: 99%

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

et al. 2014

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“…A 300 nm silicon nitride passivation layer was then deposited. On top of the passivated sensors, ten-gold conducting MCs were fabricated using photolithography and sputtering techniques [22][23][24][25][26][27][28][29] . Each MC had a width of 10 µm and a thickness of 500 nm, and was separated by 10 µm from the nearest neighboring MC.…”

Section: Fabricationmentioning

confidence: 99%

GMR microfluidic biosensor for low concentration detection of Nanomag-D beads

et al. 2015

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This paper presents a novel microfluidic biosensor for in-vitro detection of biomolecules labeled by magnetic biomarkers (Nanomag-D beads) suspended in a static fluid in combination with giant magnetoresistance (GMR) sensors. While previous studies were focused mainly on exploring the MR change for biosensing of bacteria labeled with magnetic microparticles, we show that our biosensor can be used for the detection of much smaller pathogens in the range of a few hundred nanometers e.g., viruses labeled with Nanomag-D beads (MNPs). For the measurements we also used a novel method for signal acquisition and demodulation. Expensive function generators, data acquisition devices and lock-in amplifiers are substituted by a generic PC sound card and an algorithm combining the Fast Fourier Transform (FFT) of the signal with a peak detection routine. This way, costs are drastically reduced, portability is enabled, detection handson time is reduced, and sample throughput can be increased using automation and efficient data evaluation with the appropriate software.

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“…Researchers from the Vienna University of Technology in cooperation with the King Abdullah University of Science and Technology introduced a method utilizing microfludics and MNPs which does not rely on functionalization of the sensor surface [32,75,76]. This biosensing system utilizes the innovative concept of the velocity dependence of MNPs due to their volumetric change when bioanalyte is attached to their surface via antibody-antigen binding (see Figure 5).…”

Section: Microfluidic Biosensing Systems: State Of the Artmentioning

confidence: 99%

Microfluidic Biosensing Systems Using Magnetic Nanoparticles

Giouroudi

Keplinger

2013

IJMS

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In recent years, there has been rapidly growing interest in developing hand held, sensitive and cost-effective on-chip biosensing systems that directly translate the presence of certain bioanalytes (e.g., biomolecules, cells and viruses) into an electronic signal. The impressive and rapid progress in micro- and nanotechnology as well as in biotechnology enables the integration of a variety of analytical functions in a single chip. All necessary sample handling and analysis steps are then performed within the chip. Microfluidic systems for biomedical analysis usually consist of a set of units, which guarantees the manipulation, detection and recognition of bioanalytes in a reliable and flexible manner. Additionally, the use of magnetic fields for performing the aforementioned tasks has been steadily gaining interest. This is because magnetic fields can be well tuned and applied either externally or from a directly integrated solution in the biosensing system. In combination with these applied magnetic fields, magnetic nanoparticles are utilized. Some of the merits of magnetic nanoparticles are the possibility of manipulating them inside microfluidic channels by utilizing high gradient magnetic fields, their detection by integrated magnetic microsensors, and their flexibility due to functionalization by means of surface modification and specific binding. Their multi-functionality is what makes them ideal candidates as the active component in miniaturized on-chip biosensing systems. In this review, focus will be given to the type of biosening systems that use microfluidics in combination with magnetoresistive sensors and detect the presence of bioanalyte tagged with magnetic nanoparticles.

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On-chip bio-analyte detection utilizing the velocity of magnetic microparticles in a fluid

Cited by 11 publications

References 14 publications

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

GMR microfluidic biosensor for low concentration detection of Nanomag-D beads

Microfluidic Biosensing Systems Using Magnetic Nanoparticles

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