On-chip culturing of hepatocytes and monitoring their ammoniametabolism

Satoh, Wataru; Takahashi, Shintaro; Sassa, Fumihiro; Fukuda, Junji; Suzuki, Hiroaki

doi:10.1039/b810961c

Cited by 28 publications

(12 citation statements)

References 13 publications

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“…Processing of solutions on the device for the monitoring of ammonia metabolism of hepatocytes . [Color figure can be viewed at wileyonlinelibrary.com]…”

Section: Measurement Of Ammonia Metabolic Rate Of Hepatocytesmentioning

confidence: 99%

“…Monitoring of ammonia metabolism of hepatocytes . Hepatocytes were seeded at densities of 11 cells/mm 2 (○) or 115 cells/mm 2 (□) in the microsystem.…”

Section: Measurement Of Ammonia Metabolic Rate Of Hepatocytesmentioning

confidence: 99%

“…10. Processing of solutions on the device for the monitoring of ammonia metabolism of hepatocytes [14].…”

Section: Stress Evaluation By Measuring Neutrophil Activitymentioning

confidence: 99%

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Review of Microfabricated Electrochemical Devices for the Analysis of Cell Functions and Biological Samples

Koji

Yoshizumi

Tsuchiya

et al. 2017

Elect Comm in Japan

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SUMMARY There is a strong interest in onsite rapid analysis and monitoring of the activity of cells and biological samples. Such analyses contribute not only to the understanding of cell functions, but also to the improvement of point‐of‐care testing and quality‐of‐life. In promoting miniaturization and integration of sensing components, electrochemistry is advantageous. By coupling this technique with microfluidic techniques, additional functions that can never be realized with only independent sensors can be realized. In this review paper, we introduce our recent electrochemical devices, particularly focusing on analyses of cell functions and biological samples.

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“…Processing of solutions on the device for the monitoring of ammonia metabolism of hepatocytes . [Color figure can be viewed at wileyonlinelibrary.com]…”

Section: Measurement Of Ammonia Metabolic Rate Of Hepatocytesmentioning

confidence: 99%

“…Monitoring of ammonia metabolism of hepatocytes . Hepatocytes were seeded at densities of 11 cells/mm 2 (○) or 115 cells/mm 2 (□) in the microsystem.…”

Section: Measurement Of Ammonia Metabolic Rate Of Hepatocytesmentioning

confidence: 99%

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Review of Microfabricated Electrochemical Devices for the Analysis of Cell Functions and Biological Samples

Koji

Yoshizumi

Tsuchiya

et al. 2017

Elect Comm in Japan

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“…Fully integrated µTAS devices are capable of both exposing or stimulating the cells and measuring the inherent metabolic response. A microfluidic device for monitoring ammonia metabolism from hepatocytes cultured on-chip was presented by Satoh et al [70]. An iridium oxide pH-indicator electrode and Ag/AgCl reference electrode positioned at an air gap junction within the glass-PDMS microfluidic device served as the ammonia sensor.…”

Section: Microfluidics For Cellular Analysismentioning

confidence: 99%

Review: Microfluidic applications in metabolomics and metabolic profiling

Kraly

Holcomb

Guan

et al. 2009

Analytica Chimica Acta

120

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Metabolomics is an emerging area of research focused on measuring small molecules in biological samples. There are a number of different types of metabolomics, ranging from global profiling of all metabolites in a single sample to measurement of a selected group of analytes. Microfluidics and related technologies have been used in this research area with good success. The aim of this review article is to summarize the use of microfluidics in metabolomics. Direct application of microfluidics to the determination of small molecules is covered first. Next, important sample preparation methods developed for microfluidics and applicable to metabolomics are covered. Finally, a summary of metabolomic work as it relates to analysis of cellular events using microfluidics is covered. KeywordsMicrofluidics; metabolomics; metabolic profiling; review I INTRODUCTIONThe goal of this review is to summarize the use of microfluidics as a novel tool set for metabolomics, metabolic profiling, and other metabolite related biological studies. To the knowledge of the authors this is the first such review generated, and thus we will include articles dating back as far as 2000 to give the reader a comprehensive view of the state of the art. While this is the first review of this topic, a number of important reviews on topics ranging from microfluidic and general analytical methods applicable to metabolomics [1][2][3][4][5][6] to metabolomic and metabolic profiling [7][8][9][10][11][12] have been published previously. For specific topics, it is suggested the reader consult these works. These reviews are by no means a comprehensive list of all reviews in this field given that several dozen review articles have been published over the last nine years ranging in scope from very specific to more general.Before discussing specific applications of microfluidics to metabolomics we will first define metabolomics and the various subsets of this discipline. While many people are familiar with genomics and proteomics, the terminology associated with metabolomics is not as clearly defined. For the sake of this review, we define metabolomics as five separate categories: 1) metabolomics, 2) metabonomics, 3) metabolic fingerprinting, 4) metabolic profiling, and 5) targeted metabolic profiling [13]. Metabolomics is the overall study of metabolite expression Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Author ManuscriptAnal Chim Acta. Author manuscript; available in PMC 2010 October 19. Published in final edited form as:Anal Chim Acta. NIH-PA Author ManuscriptNIH-PA Author Manuscript ...

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“…Miniaturized systems offer many potential advantages over conventional assay platforms, including small sample volumes, low cost, short assay time, high throughput, and automation [3,4]. Systems with integrated electrochemical sensors, in particular, could provide new opportunities in bedside diagnosis and assays for use at home, because of their simplicity and ability to function without expensive equipment [5].…”

Section: Introductionmentioning

confidence: 99%

Synergistic effects of micro/nano modifications on electrodes for microfluidic electrochemical ELISA

Numthuam

Kakegawa

Anada

et al. 2011

Sensors and Actuators B: Chemical

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Microfluidic electrochemical sensing has been considered to be highly efficient. In this study, we showed, by using numerical simulations, that a planar electrode formed on the bottom of a microchannel is exposed to only a small fraction of analytes in amperometric detection. We also showed that three-dimensional (3D) micropillar electrodes significantly improve the detection current. The practical performance was evaluated using 3D micropillar electrodes fabricated by photolithography. The output current increased as the diameters of the micropillars decreased, as predicted by the simulations. It is noteworthy that the current enhancements obtained with the 3D electrodes were larger than those expected from an increase in the surface area. Further increase in current was achieved by electrical deposition of nanoporous gold-black onto the surface of the 3D electrode: when a 3D electrode with micropillars 30 µm in diameter was used, the output current was approximately 20 times that obtained with a 2D electrode without modification. The applicability of the micropillar electrodes was demonstrated in electrochemical enzyme-linked immunosorbent assay (ELISA) of bone metabolic marker proteins. Although an increase in the surface area of the electrode leads to more noise in general, there is no significant difference in the signal-to-noise ratio between the modified 3D electrode and the 2D electrode without modification in the ELISA experiments. This nanoporous micropillar electrode could potentially be a useful component for the development of on-site diagnosis systems.

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On-chip culturing of hepatocytes and monitoring their ammoniametabolism

Abstract: Ammonia metabolism of hepatocytes was monitored at nanolitre-scale volumes of a medium in a microsystem with microfluidic and sensing functions.

Cited by 28 publications

References 13 publications

Review of Microfabricated Electrochemical Devices for the Analysis of Cell Functions and Biological Samples

Review of Microfabricated Electrochemical Devices for the Analysis of Cell Functions and Biological Samples

Review: Microfluidic applications in metabolomics and metabolic profiling

Synergistic effects of micro/nano modifications on electrodes for microfluidic electrochemical ELISA

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