On chip detection of glial cell-derived neurotrophic factor secreted from dopaminergic cells under magnetic stimulation

Yang, Renyuan; Boldrey, Joseph; Jiles, David; Schneider, Ian C.; Que, Long

doi:10.1016/j.bios.2021.113179

Cited by 4 publications

(4 citation statements)

References 28 publications

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“…After that, the LDH antigens were added to the sensor. Then the second spectrum was recorded after 30 min incubation at room temperature followed by a rigorous phosphate-buffered saline (PBS) rinsing. , The operational principle of the sensor was reported in our previous publication . The peak shift (nm) of the interference fringes was obtained from those spectra as the transducing signal, which is summarized in Figure S2A,B in the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

“…The nanopore sensor was characterized by measuring the transducing signals of LDH samples of known concentrations. First, the sensor surface was functionalized by the LDH antibody using the protocol developed in our lab. , Then the optical spectrum was recorded using a spectrometer (OceanOptics, Inc.). After that, the LDH antigens were added to the sensor.…”

Section: Methodsmentioning

confidence: 99%

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Assessment of the Behaviors of an In Vitro Brain Model On-Chip under Shockwave Impacts

Rubby,

Fonder,

Uchayash

et al. 2024

ACS Appl. Mater. Interfaces

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Herein we report the assessment of the effects of shockwave (SW) impacts on adult rat hippocampal progenitor cell (AHPC) neurospheres (NSs), which are used as in vitro brain models, for enhancing our understanding of the mechanisms of traumatic brain injury (TBI). The assessment has been achieved by using culture dishes and a new microchip. The microchip allows the chemicals released from the brain models cultured inside the cell culture chamber under SW impacts to diffuse to the nanosensors in adjacent sensor chambers through built-in diffusion barriers, which are used to prevent the cells from entering the sensor chambers, thereby mitigating the biofouling issues of the sensor surface. Experiments showed the negative impact of the SW on the viability, proliferation, and differentiation of the cells within the NSs. A qPCR gene expression analysis was performed and appeared to confirm some of the immunocytochemistry (ICC) results. Finally, we demonstrated that the microchip can be used to monitor lactate dehydrogenase (LDH) released from the AHPC-NSs subjected to SW impacts. As expected, LDH levels changed when AHPC-NSs were injured by SW impacts, verifying this chip can be used for assessing the degrees of injuries to AHPC-NSs by monitoring LDH levels. Taken together, these results suggest the feasibility of using the chip to better understand the interactions between SW impacts and in vitro brain models, paving the way for potentially establishing in vitro TBI models on a chip.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Assessment of the Behaviors of an In Vitro Brain Model On-Chip under Shockwave Impacts

Rubby,

Fonder,

Uchayash

et al. 2024

ACS Appl. Mater. Interfaces

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“…An ultrasensitive nanosensor consisting of a Au-coated nanopore thin film was reported by Yang et al in 2021 [132]. This nanosensor was developed for the detection of N27 cells' Glial cell-derived neurotrophic factor (GDNF) secretion [132]. The GDNF is a small protein that strongly promotes the survival of dopaminergic and motor neurons, and the evaluation of its magnetic stimulation is valuable.…”

Section: Gold and Silver Nanoparticle-based Optical Sensorsmentioning

confidence: 99%

“…The time-dependent release of three different osteogenic differentiation markers (ALP, osteocalcin, and fibronectin) were detected up to the pg/mL levels without interference or crosstalk for three An ultrasensitive nanosensor consisting of a Au-coated nanopore thin film was reported by Yang et al in 2021 [132]. This nanosensor was developed for the detection of N27 cells' Glial cell-derived neurotrophic factor (GDNF) secretion [132]. The GDNF is a small protein that strongly promotes the survival of dopaminergic and motor neurons, and the evaluation of its magnetic stimulation is valuable.…”

Section: Gold and Silver Nanoparticle-based Optical Sensorsmentioning

confidence: 99%

Progress in Nano-Biosensors for Non-Invasive Monitoring of Stem Cell Differentiation

2023

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Non-invasive, non-destructive, and label-free sensing techniques are required to monitor real-time stem cell differentiation. However, conventional analysis methods, such as immunocytochemistry, polymerase chain reaction, and Western blot, involve invasive processes and are complicated and time-consuming. Unlike traditional cellular sensing methods, electrochemical and optical sensing techniques allow non-invasive qualitative identification of cellular phenotypes and quantitative analysis of stem cell differentiation. In addition, various nano- and micromaterials with cell-friendly properties can greatly improve the performance of existing sensors. This review focuses on nano- and micromaterials that have been reported to improve sensing capabilities, including sensitivity and selectivity, of biosensors towards target analytes associated with specific stem cell differentiation. The information presented aims to motivate further research into nano-and micromaterials with advantageous properties for developing or improving existing nano-biosensors to achieve the practical evaluation of stem cell differentiation and efficient stem cell-based therapies.

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In situ monitoring of neurotransmitters using a polymer nanostructured electrochemical sensing microchip

Rubby,

Fonder,

Uchayash

et al. 2024

Microchemical Journal

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On chip detection of glial cell-derived neurotrophic factor secreted from dopaminergic cells under magnetic stimulation

Cited by 4 publications

References 28 publications

Assessment of the Behaviors of an In Vitro Brain Model On-Chip under Shockwave Impacts

Assessment of the Behaviors of an In Vitro Brain Model On-Chip under Shockwave Impacts

Progress in Nano-Biosensors for Non-Invasive Monitoring of Stem Cell Differentiation

In situ monitoring of neurotransmitters using a polymer nanostructured electrochemical sensing microchip

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