On-chip fabrication of movable toroidal cell structures using photo-crosslinkable biodegradable hydrogel

Takeuchi, Masaru; Nakamura, Yuki; Ichikawa, Akihiko; Hasegawa, Akira; Hasegawa, Yasuhisa; Fukuda, Toshio

doi:10.1109/iros.2017.8206134

Cited by 4 publications

(6 citation statements)

References 21 publications

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“…The GelMA was synthesized as follows [22][23][24][25]. First, type-A porcine skin gelatin was added at 10% w/v to phosphate-buffered saline (PBS) and heated at 50 • C for 1 h with stirring.…”

Section: Preparation Of Biodegradable Microstructuresmentioning

confidence: 99%

“…In our method [22], cells were mixed in GelMA solution and encapsulated inside the GelMA microstructures by exposing them to patterned UV radiation. Such on-chip fabrication can be achieved by using patterned UV light through a mask.…”

Section: Cell Culture In Gelma Microstructuresmentioning

confidence: 99%

“…Such on-chip fabrication can be achieved by using patterned UV light through a mask. However, when the cells are encapsulated inside GelMA microstructures, only a few cells are stretched and grown [22]. In this study, we created cell cultures inside and on the GelMA microstructures to check the differences of cell growth.…”

Section: Cell Culture In Gelma Microstructuresmentioning

confidence: 99%

“…In our method, 2D toroidal microstructures were used to assemble 3D tube shapes. The fabricated microstructures were movable when the water-repellent coating was applied to the surface of the microchannel [22]. The fabrication and assembly of microstructures could thus be conducted using the same microfluidic chip.…”

Section: Movable Gelma Microstructures In a Microfluidic Devicementioning

confidence: 99%

“…The microfluidic chip was made of polydimethylsiloxane (PDMS) and fabricated using a general soft-lithography process. The channel was coated by a water-repellent coating material to peel off the microstructures after fabrication [22]. In the microchannel, toroidal GelMA microstructures were fabricated using patterned UV light.…”

Section: Movable Gelma Microstructures In a Microfluidic Devicementioning

confidence: 99%

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On-Chip Fabrication of Cell-Attached Microstructures using Photo-Cross-Linkable Biodegradable Hydrogel

Takeuchi

Kozuka

Kim

et al. 2020

JFB

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We developed a procedure for fabricating movable biological cell structures using biodegradable materials on a microfluidic chip. A photo-cross-linkable biodegradable hydrogel gelatin methacrylate (GelMA) was used to fabricate arbitrary microstructure shapes under a microscope using patterned ultraviolet light. The GelMA microstructures were movable inside the microfluidic channel after applying a hydrophobic coating material. The fabricated microstructures were self-assembled inside the microfluidic chip using our method of fluid forcing. The synthesis procedure of GelMA was optimized by changing the dialysis temperature, which kept the GelMA at a suitable pH for cell culture. RLC-18 rat liver cells (Riken BioResource Research Center, Tsukuba, Japan) were cultured inside the GelMA and on the GelMA microstructures to check cell growth. The cells were then stretched for 1 day in the cell culture and grew well on the GelMA microstructures. However, they did not grow well inside the GelMA microstructures. The GelMA microstructures were partially dissolved after 4 days of cell culture because of their biodegradability after the cells were placed on the microstructures. The results indicated that the proposed procedure used to fabricate cell structures using GelMA can be used as a building block to assemble three-dimensional tissue-like cell structures in vitro inside microfluidic devices.

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Section: Preparation Of Biodegradable Microstructuresmentioning

confidence: 99%

Section: Cell Culture In Gelma Microstructuresmentioning

confidence: 99%

Section: Cell Culture In Gelma Microstructuresmentioning

confidence: 99%

Section: Movable Gelma Microstructures In a Microfluidic Devicementioning

confidence: 99%

Section: Movable Gelma Microstructures In a Microfluidic Devicementioning

confidence: 99%

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