On-Chip Fluorescence-Activated Cell Sorting by an Integrated Miniaturized Ultrasonic Transducer

Johansson, Lotta; Nikolajeff, Fredrik; Johansson, Stefan; Thorslund, Sara

doi:10.1021/ac802681r

Cited by 70 publications

(56 citation statements)

References 35 publications

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“…Microfluidic platforms have been utilized for several years to improve efficiency, accessibility, and flexibility of systems designed to purify small particles [15][16][17][18][19] and have been utilized more recently to purify large particles based on particle size. 20,21 In particular, a microfluidic 1 mm glass capillary sorting mechanism was developed using diode laser bars to optically trap or deflect particles up to 200 lm in diameter.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic sorting of microtissues

et al. 2012

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Increasingly, in vitro culture of adherent cell types utilizes three-dimensional (3D) scaffolds or aggregate culture strategies to mimic tissue-like, microenvironmental conditions. In parallel, new flow cytometry-based technologies are emerging to accurately analyze the composition and function of these microtissues (i.e., large particles) in a non-invasive and high-throughput way. Lacking, however, is an accessible platform that can be used to effectively sort or purify large particles based on analysis parameters. Here we describe a microfluidic-based, electromechanical approach to sort large particles. Specifically, sheath-less asymmetric curving channels were employed to separate and hydrodynamically focus particles to be analyzed and subsequently sorted. This design was developed and characterized based on wall shear stress, tortuosity of the flow path, vorticity of the fluid in the channel, sorting efficiency and enrichment ratio. The large particle sorting device was capable of purifying fluorescently labelled embryoid bodies (EBs) from unlabelled EBs with an efficiency of 87.3% 6 13.5%, and enrichment ratio of 12.2 6 8.4 (n ¼ 8), while preserving cell viability, differentiation potential, and long-term function. V C 2012 American Institute of Physics. [http://dx

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Section: Introductionmentioning

confidence: 99%

Microfluidic sorting of microtissues

et al. 2012

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“…For the silicon-based devices, fabrication through standard photolithography and wet or dry etching are common approaches [61,94,69,95,47,96]. In the case of polymers and particularly PDMS devices, standard soft lithography methods [87,89,93] and rapid prototyping are commonly employed [97]. Typically, a transparent material with higher acoustic impedance such as glass or fused silica is used as a lid to form the channel.…”

Section: Materials and Fabricationmentioning

confidence: 99%

“…Typically, the power required for ACT applications is in the order of couple of Watts. However, in some high throughput studies with PDMS materials, it can go up to 10 W [97] which requires an additional external cooling of the piezoelectric material and the channel. The use of aluminum block as a heat sink may be applied [85,97,110].…”

Section: Hardwarementioning

confidence: 99%

Microfluidic bio-particle manipulation for biotechnology

Çetin

Özer

Solmaz

2014

Biochemical Engineering Journal

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a b s t r a c tMicrofluidics and lab-on-a-chip technology offers unique advantages for the next generation devices for diagnostic therapeutic applications. For chemical, biological and biomedical analysis in microfluidic systems, there are some fundamental operations such as separation, focusing, filtering, concentration, trapping, detection, sorting, counting, washing, lysis of bio-particles, and PCR-like reactions. The combination of these operations led to the complete analysis systems for specific applications. Manipulation of the bio-particles is the key ingredient for these applications. Therefore, microfluidic bio-particle manipulation has attracted a significant attention from the academic community. Considering the size of the bio-particles and the throughput of the practical applications, manipulation of the bio-particles is a challenging problem. Different techniques are available for the manipulation of bio-particles in microfluidic systems. In this review, some of the techniques for the manipulation of bio-particles; namely hydrodynamic based, electrokinetic-based, acoustic-based, magnetic-based and optical-based methods have been discussed. The comparison of different techniques and the recent applications regarding the microfluidic bio-particle manipulation for different biotechnology applications are presented. Finally, challenges and the future research directions for microfluidic bio-particle manipulation are addressed.

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“…The deflection can be tuned by controlling the magnitude and waveform of the input voltage, and sorting of more than 1,000 particles/s can be achieved. Johansson et al (136) have also sorted cells using an piezoelectric transducer on the microfluidic channel bottom. In this case, the acoustic radiation force acts on the interface between two fluids of different density, so that large particle displacements, up to 100 lm for a 3 MHz system can be achieved, although the switch time of 0.36 s corresponds to a sorting speed of 3 particles/s.…”

Section: Future Challengesmentioning

confidence: 99%

Microfluidic impedance‐based flow cytometry

Berardino²,

et al. 2010

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Microfabricated flow cytometers can detect, count, and analyze cells or particles using microfluidics and electronics to give impedance-based characterization. Such systems are being developed to provide simple, low-cost, label-free, and portable solutions for cell analysis. Recent work using microfabricated systems has demonstrated the capability to analyze micro-organisms, erythrocytes, leukocytes, and animal and human cell lines. Multifrequency impedance measurements can give multiparametric, high-content data that can be used to distinguish cell types. New combinations of microfluidic sample handling design and microscale flow phenomena have been used to focus and position cells within the channel for improved sensitivity. Robust designs will enable focusing at high flowrates while reducing requirements for control over multiple sample and sheath flows. Although microfluidic impedance-based flow cytometers have not yet or may never reach the extremely high throughput of conventional flow cytometers, the advantages of portability, simplicity, and ability to analyze single cells in small populations are, nevertheless, where chip-based cytometry can make a large impact. ' 2010International Society for Advancement of Cytometry Key terms microfluidics; impedance characterization; label free; single cell analysis; hydrodynamic focusing; sorting MICROFLUIDIC flow cytometers can bring many advantages to the field of flow cytometry (FCM). Compared to typical flow cytometry channel sizes, the miniaturized dimensions permit microfluidic systems to analyze single cells, to identify cellular variability in gene expression, or drug response within a cell population. Chipbased cytometers can have lower size and costs than conventional benchtop instruments, and may be portable. Today, the developmental aim for microfluidic systems is to reach the same sensitivity and capability for multiparametric analyses as delivered by conventional flow cytometers. Many efforts have been made to improve existing devices and to create new miniaturized high-end instruments. Microfluidic chips can incorporate on-chip cell preparation, cell culture, lysis, and modules for optical, electrophoretic, or genomic analysis (1). They are also suitable for analysis of cells in suspension as well as adherent cells.Miniaturized cytometry devices will have high impact in the development of point-of-care devices in developing countries. Accurate CD4 1 T-cell counts are used to monitor human immunodeficiency virus (HIV)-infected patients, and various thresholds of the number of CD4 1 T lymphocytes per ll of whole blood are used to start antiretroviral therapy (2-5). A simple, single-purpose CD4 cell counting device, which does not require standard laboratory equipment or trained laboratory personnel, could help some of the 33 million HIV-infected people worldwide monitor the stage of infection (6)(7)(8). In this application, increased analysis throughput is a secondary concern compared to increased sensitivity and specificity. Portable, miniatu...

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On-Chip Fluorescence-Activated Cell Sorting by an Integrated Miniaturized Ultrasonic Transducer

Abstract: An acoustic microfluidic system for miniaturized fluorescence activated cell sorting (µ FACS) is presented.

Cited by 70 publications

References 35 publications

Microfluidic sorting of microtissues

Microfluidic sorting of microtissues

Microfluidic bio-particle manipulation for biotechnology

Microfluidic impedance‐based flow cytometry

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