On-chip magneto-immunoassay for Alzheimer's biomarker electrochemical detection by using quantum dots as labels

Medina‐Sánchez, Mariana; Miserere, Sandrine; Morales‐Narváez, Eden; Merkoçi, Arben

doi:10.1016/j.bios.2013.10.069

Cited by 100 publications

(57 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The obtained results are in concordance with the above mentioned expected data and also with those previously obtained in our group using both microarray technology [50] and electrochemical detection [51] based on commercial Quantum Dot tags.…”

Section: Special Issue Diagnosticssupporting

confidence: 92%

Alzheimer Disease Biomarker Detection Through Electrocatalytic Water Oxidation Induced by Iridium Oxide Nanoparticles

et al. 2014

Self Cite

View full text Add to dashboard Cite

Iridium oxide nanoparticles (IrO2 NPs) synthesized following a previously reported chemical route are presented as novel tags for immunosensing taking advantage of their electrocatalytic activity towards water oxidation reaction (WOR). Cyclic voltammetry and chronoamperometry for the evaluation of the IrO2 NPs electrocatalytic activity towards WOR at neutral pH were used. The chronoamperometric current recorded at a fixed potential of +1.3 V constituted the analytical signal allowing the quantification of IrO2 NPs at nM levels. Modification of the surface of citrate‐capped IrO2 NPs with anti‐Apolipoprotein E antibodies (αApoE) was successfully achieved and the as‐prepared conjugates were used for the electrocatalytic detection of ApoE Alzheimer disease (AD) biomarker in a magnetosandwich immunoassay, reaching a detection limit of 68 ng/mL. Human plasma of a patient suffering AD was also evaluated, estimating an ApoE concentration of 20 µg/mL which is in concordance with the obtained in previously reported approaches. This novel IrO2 NPs based electrocatalytic assay presents the advantage of the signal generation in the same medium where the immunoassay takes place (PBS, pH 7.4) avoiding the use of additional reagents which also opens the way to future integrated biosensing systems and platforms with interest for other proteins as well as DNA and cells analysis.

show abstract

Section: Special Issue Diagnosticssupporting

confidence: 92%

Alzheimer Disease Biomarker Detection Through Electrocatalytic Water Oxidation Induced by Iridium Oxide Nanoparticles

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…[96][97][98] One particularly interesting example of the utility of QDs also took advantage of the separation/concentrating characteristics of MNPs to develop a method to detect Mycobacterium tuberculosis (Mtb) biomarkers in the sputum of infected patients. [99] The intent was to take advantage of the optical strengths of QDs -high quantum yields and low fluorescent NPs offer many sought-after advantages in expanding the fluorescent toolbox, such as robustness, versatility, and synthetic simplicity.…”

Section: Emission-based Detectionmentioning

confidence: 99%

Advantages and limitations of nanoparticle labeling for early diagnosis of infection

Laurentius

Owens

Park

et al. 2016

Expert Review of Molecular Diagnostics

View full text Add to dashboard Cite

This review examines the advantages and limitations of in vitro health care diagnostic tests that utilize nanoparticles (e.g. noble metal, quantum dot, and magnetic). It includes a brief overview of their unique properties, syntheses, and applicable readout strategies. This is followed by a brief synopsis of the obstacles faced when attempting to translate nanoparticle-based diagnostics from the R&D laboratory to the clinic and other arenas (i.e. the difficulties common to in vitro diagnostics), and then by a much more in-depth examination of the need to control and characterize a range of nanoparticle properties (e.g. size, shape, surface composition, and stability) when making this transition. Expert commentary: The review wraps up with a short commentary and perspective for the next five years, focusing on possible guidelines for nanoparticle characterization.

show abstract

“…Quantum dots coupled to anti-Aβ antibody were designed as lab-on-chip systems to allow the screening of potential anti-aggregating compounds in a micro-scale volume of body fluid [151]. Lab-on-chip devices using cadmium/selenide/zinc sulfide QDs in order to detect the plasma levels of ApoE ex vivo as a diagnosis biomarker [152]. Some other NP based systems were described for biomarker detection such as tau protein [153].…”

Section: Therapeutic Strategies Using Nanoparticles In Ndmentioning

confidence: 99%

The Ins and Outs of Nanoparticle Technology in Neurodegenerative Diseases and Cancer

Wilson¹,

Magnaudeix²,

Naves³

et al. 2015

CDM

View full text Add to dashboard Cite

On-chip magneto-immunoassay for Alzheimer's biomarker electrochemical detection by using quantum dots as labels

Cited by 100 publications

References 30 publications

Alzheimer Disease Biomarker Detection Through Electrocatalytic Water Oxidation Induced by Iridium Oxide Nanoparticles

Alzheimer Disease Biomarker Detection Through Electrocatalytic Water Oxidation Induced by Iridium Oxide Nanoparticles

Advantages and limitations of nanoparticle labeling for early diagnosis of infection

The Ins and Outs of Nanoparticle Technology in Neurodegenerative Diseases and Cancer

Contact Info

Product

Resources

About