On-chip microlasers for biomolecular detection via highly localized deposition of a multifunctional phospholipid ink

Bog, Uwe; Laue, Thomas M.; Großmann, Tobias; Beck, Torsten; Wienhold, Tobias; Richter, Benjamin; Hirtz, Michael; Fuchs, Harald; Kalt, H.; Mappes, Timo

doi:10.1039/c3lc50149c

Cited by 54 publications

(41 citation statements)

References 33 publications

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“…Examples of this work include phospholipid modification of microgoblet arrays for investigating lipid binding or other lipid-biomolecule interactions (80, 127). Lipid bilayer assembly dynamics were observed by monitoring bilayer formation in real time (128).…”

Section: Applications Of Optical Resonatorsmentioning

confidence: 99%

Applications of Optical Microcavity Resonators in Analytical Chemistry

Wade

Bailey

2016

Annual Rev. Anal. Chem.

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Optical resonator sensors are an emerging class of analytical technologies that use recirculating light confined within a microcavity to sensitively measure the surrounding environment. Bolstered by advances in microfabrication, these devices can be configured for a wide variety of chemical or biomolecular sensing applications. The review begins with a brief description of optical resonator sensor operation followed by discussions regarding sensor design, including different geometries, choices of material systems, methods of sensor interrogation, and new approaches to sensor operation. Throughout, key recent developments are highlighted, including advancements in biosensing and other applications of optical sensors. Alternative sensing mechanisms and hybrid sensing devices are then discussed in terms of their potential for more sensitive and rapid analyses. Brief concluding statements offer our perspective on the future of optical microcavity sensors and their promise as versatile detection elements within analytical chemistry.

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Section: Applications Of Optical Resonatorsmentioning

confidence: 99%

Applications of Optical Microcavity Resonators in Analytical Chemistry

Wade

Bailey

2016

Annual Rev. Anal. Chem.

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“…To mitigate this issue, biomaterials are typically applied to completed or nearly completed devices. New advances in fabrication processes, such as soft lithography [130,165,182], dip-pen lithography [122], inkjet printing [157,183], and development of ultraviolet-patternable biomaterials [157,182] will further enable fabrication of improved optical sensors with biomaterials.…”

Section: Hybrid Sensing Devicesmentioning

confidence: 99%

Hybrid Integrated Label-Free Chemical and Biological Sensors

Mehrabani

Maker

Armani

2014

Sensors

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Label-free sensors based on electrical, mechanical and optical transduction methods have potential applications in numerous areas of society, ranging from healthcare to environmental monitoring. Initial research in the field focused on the development and optimization of various sensor platforms fabricated from a single material system, such as fiber-based optical sensors and silicon nanowire-based electrical sensors. However, more recent research efforts have explored designing sensors fabricated from multiple materials. For example, synthetic materials and/or biomaterials can also be added to the sensor to improve its response toward analytes of interest. By leveraging the properties of the different material systems, these hybrid sensing devices can have significantly improved performance over their single-material counterparts (better sensitivity, specificity, signal to noise, and/or detection limits). This review will briefly discuss some of the methods for creating these multi-material sensor platforms and the advances enabled by this design approach.

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“…Advances in biolasers have demonstrated great potential in biosensing, biomedical research, and diagnosis 4,11,20-22,24 due to their capability to amplify subtle changes in the gain media caused by underlying biological processes, which, in combination of threshold behavior, narrow linewidth, strong lasing emission, and lasing mode spatial distribution, may lead to significant increase in detection sensitivity, multiplexibility, and imaging contrast 8,16,25,26 . Over the past few years, biolasers have been focused mainly on the molecular level and shown the significantly improved sensitivity in detecting biomolecules and their structural changes 3,9,27-30 . More recently, biolasers using single cells with fluorescent proteins inside or externally labelled dyes/beads as the gain medium have been applied to single cell analysis 4,12-15 .…”

Section: Introductionmentioning

confidence: 99%

Versatile tissue lasers based on high-Q Fabry–Pérot microcavities

Chen

Zhang

et al. 2017

Lab Chip

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Biolasers are an emerging technology for next generation biochemical detection and clinical applications. Progress has recently been made to achieve lasing from biomolecules and single living cells. Tissues, which consist of cells embedded in extracellular matrix, mimic more closely the actual complex biological environment in a living body and therefore are of more practical significance. Here, we developed a highly versatile tissue laser platform, in which tissues stained with fluorophores are sandwiched in a high-Q Fabry-Pérot microcavity. Distinct lasing emissions from muscle and adipose tissues stained respectively with fluorescein isothiocyanate (FITC) and boron-dipyrromethene (BODIPY), and hybrid muscle/adipose tissue with dual-staining were achieved with a threshold of only ~10 μJ/mm2. Additionally, we investigated how tissue structure/geometry, tissue thickness, and staining dye concentration affect the tissue laser. Lasing emission from FITC conjugates (FITC-phalloidin) that target specifically F-actin in muscle tissues was also realized. It is further found that, despite large fluorescence spectral overlap between FITC and BODIPY in tissues, their lasing emissions could be clearly distinguished and controlled due to their narrow lasing bands and different lasing thresholds, thus enabling highly multiplexed detection. Our tissue laser platform can be broadly applicable to various types of tissues/diseases. It provides a new tool for a wide range of biological and biomedical applications, such as diagnostics/screening of tissues and identification/monitoring of biological transformations in tissue engineering.

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On-chip microlasers for biomolecular detection via highly localized deposition of a multifunctional phospholipid ink

Cited by 54 publications

References 33 publications

Applications of Optical Microcavity Resonators in Analytical Chemistry

Applications of Optical Microcavity Resonators in Analytical Chemistry

Hybrid Integrated Label-Free Chemical and Biological Sensors

Versatile tissue lasers based on high-Q Fabry–Pérot microcavities

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