On-chip modeling of tumor evolution: Advances, challenges and opportunities

Li, Chengpan; Holman, Joseph Benjamin; Shi, Zhengdi; Qiu, Bensheng; Ding, Weiping

doi:10.1016/j.mtbio.2023.100724

Cited by 11 publications

(3 citation statements)

References 288 publications

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“…Regarding the simulation of mechanical stimuli on microchips, modeling shear stress conditions requires the use of microfluidic channels, which can be sensitive to clogging and fouling, while optimizing flow conditions or the level of solid stress to minimize their effect on cell survival and functionality is essential. 166 Regarding cell isolation, it should be noted that microfluidic devices and microchips usually require small sample volumes, making it challenging to extract enough cells for downstream analysis, such as proteomics or transcriptomics. Scaling up microfluidic processes for high-throughput applications or ensuring that the microfluidic device can handle larger sample volumes without compromising the experimental success should be considered.…”

Section: Conclusive Remarks and Remaining Challengesmentioning

confidence: 99%

Toward innovative approaches for exploring the mechanically regulated tumor-immune microenvironment

Kalli,

Stylianopoulos

2024

APL Bioengineering

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Within the complex tumor microenvironment, cells experience mechanical cues—such as extracellular matrix stiffening and elevation of solid stress, interstitial fluid pressure, and fluid shear stress—that significantly impact cancer cell behavior and immune responses. Recognizing the significance of these mechanical cues not only sheds light on cancer progression but also holds promise for identifying potential biomarkers that would predict therapeutic outcomes. However, standardizing methods for studying how mechanical cues affect tumor progression is challenging. This challenge stems from the limitations of traditional in vitro cell culture systems, which fail to encompass the critical contextual cues present in vivo. To address this, 3D tumor spheroids have been established as a preferred model, more closely mimicking cancer progression, but they usually lack reproduction of the mechanical microenvironment encountered in actual solid tumors. Here, we review the role of mechanical forces in modulating tumor- and immune-cell responses and discuss how grasping the importance of these mechanical cues could revolutionize in vitro tumor tissue engineering. The creation of more physiologically relevant environments that better replicate in vivo conditions will eventually increase the efficacy of currently available treatments, including immunotherapies.

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Section: Conclusive Remarks and Remaining Challengesmentioning

confidence: 99%

Toward innovative approaches for exploring the mechanically regulated tumor-immune microenvironment

Kalli,

Stylianopoulos

2024

APL Bioengineering

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“…Moreover, chemical gradients affect diverse cellular behaviors such as migration, proliferation, differentiation, inflammation, or tumor evolution. [17][18][19][20][21] For example, oxygen gradients are also related to cell growth, survival, and migration. [22][23][24] Thus, producing controlled gradients in OOC systems is essential to obtain biomimetic environments and to evaluate those cellular processes in the OOC technology.…”

Section: Introductionmentioning

confidence: 99%

Tuneable hydrogel patterns in pillarless microfluidic devices

Olaizola-Rodrigo,

Palma-Florez,

Ranđelović

et al. 2024

Lab Chip

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“…Tumors are malignant diseases that pose a serious threat to human health. Tumor cells have the ability to proliferate indefinitely and infiltrate, which can disrupt normal tissue structure and function and lead to organ failure or even death [ 1 ]. The early detection of tumors and their timely treatment can effectively improve the cure rate and survival rate.…”

Section: Introductionmentioning

confidence: 99%

Sensitive Electrochemical Detection of Carcinoembryonic Antigen Based on Biofunctionalized Nanochannel Modified Carbonaceous Electrode

Zhou,

Wang,

et al. 2024

Molecules

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The convenient construction of carbon-based electrochemical immunosensors with high performance is highly desirable for the efficient detection of tumor biomarkers. In this work, an electrochemical immunosensor was fabricated by integrating a biofunctionalized mesoporous silica nanochannel film with a carbon-based electrode, which can enable the sensitive determination of carcinoembryonic antigen (CEA) in serum. The commonly used carbonaceous electrode, glassy carbon electrode (GCE), was employed as the supporting electrode and was pre-treated through electrochemical polarization to achieve the stable binding of a vertically ordered mesoporous silica film with amino groups (NH2-VMSF) without the use of any adhesive layer. To fabricate the immunorecognition interface, antibodies were covalently immobilized after the amino groups on the outer surface of NH2-VMSF was derivatized to aldehyde groups. The presence of amino sites within the high-density nanochannels of NH2-VMSF can facilitate the migration of negatively charged redox probes (Fe(CN)63-/4-) to the supporting electrode through electrostatic adsorption, leading to the generation of electrochemical signals. In the presence of CEA, the formation of immunocomplexes on the recognitive interface can reduce the electrochemical signal of Fe(CN)63-/4- on the supporting electrode. Based on this principle, the sensitive electrochemical detection of CEA was achieved. CEA can be determined to range from 0.01 ng mL−1 to 100 ng mL−1 with a limit of detection of 6.3 pg mL−1. The fabricated immunosensor exhibited high selectivity, and the detection of CEA in fetal bovine serum was achieved.

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On-chip modeling of tumor evolution: Advances, challenges and opportunities

Cited by 11 publications

References 288 publications

Toward innovative approaches for exploring the mechanically regulated tumor-immune microenvironment

Toward innovative approaches for exploring the mechanically regulated tumor-immune microenvironment

Tuneable hydrogel patterns in pillarless microfluidic devices

Sensitive Electrochemical Detection of Carcinoembryonic Antigen Based on Biofunctionalized Nanochannel Modified Carbonaceous Electrode

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