On chip porous polymer membranes for integration of gastrointestinal tract epithelium with microfluidic ‘body-on-a-chip’ devices

Esch, Mandy B.; Sung, Jong Hwan; Yang, Jennifer H.; Chen, Yu; Yu, Jiajie; March, John C.; Shuler, Michael L.

doi:10.1007/s10544-012-9669-0

Cited by 161 publications

(128 citation statements)

References 29 publications

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“…In this way, a small cross section of gastrointestinal epithelium can be obtained as a model tissue. 129 Color images available online at www.liebertpub.com/teb 538 VRANA ET AL.…”

Section: Modular Epithelial Layers and Their Application In Organ-on-mentioning

confidence: 99%

“…128 By having porous micropillar structures, it is possible to imitate the architecture of gastrointestinal epithelium and thus obtain a reliable microscale model of drug absorption. 129 As shown in Figure 7, it is possible to obtain microvillimimicking architectures by using stereolithography techniques on membranes produced from SU-8 (an epoxy-based photoresist) on silicon micropillars. By controlling the spacing and the size of the micropillar, it is possible to control the distribution of intestinal epithelial cells.…”

Section: Modular Epithelial Layers and Their Application In Organ-on-mentioning

confidence: 99%

See 1 more Smart Citation

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Vrana

Lavalle²,

Dokmeci³

et al. 2013

Tissue Engineering Part B: Reviews

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Recent advances in the fields of microfabrication, biomaterials, and tissue engineering have provided new opportunities for developing biomimetic and functional tissues with potential applications in disease modeling, drug discovery, and replacing damaged tissues. An intact epithelium plays an indispensable role in the functionality of several organs such as the trachea, esophagus, and cornea. Furthermore, the integrity of the epithelial barrier and its degree of differentiation would define the level of success in tissue engineering of other organs such as the bladder and the skin. In this review, we focus on the challenges and requirements associated with engineering of epithelial layers in different tissues. Functional epithelial layers can be achieved by methods such as cell sheets, cell homing, and in situ epithelialization. However, for organs composed of several tissues, other important factors such as (1) in vivo epithelial cell migration, (2) multicell-type differentiation within the epithelium, and (3) epithelial cell interactions with the underlying mesenchymal cells should also be considered. Recent successful clinical trials in tissue engineering of the trachea have highlighted the importance of a functional epithelium for long-term success and survival of tissue replacements. Hence, using the trachea as a model tissue in clinical use, we describe the optimal structure of an artificial epithelium as well as challenges of obtaining a fully functional epithelium in macroscale. One of the possible remedies to address such challenges is the use of bottom-up fabrication methods to obtain a functional epithelium. Modular approaches for the generation of functional epithelial layers are reviewed and other emerging applications of microscale epithelial tissue models for studying epithelial/mesenchymal interactions in healthy and diseased (e.g., cancer) tissues are described. These models can elucidate the epithelial/mesenchymal tissue interactions at the microscale and provide the necessary tools for the next generation of multicellular engineered tissues and organ-on-a-chip systems.

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“…In this way, a small cross section of gastrointestinal epithelium can be obtained as a model tissue. 129 Color images available online at www.liebertpub.com/teb 538 VRANA ET AL.…”

Section: Modular Epithelial Layers and Their Application In Organ-on-mentioning

confidence: 99%

Section: Modular Epithelial Layers and Their Application In Organ-on-mentioning

confidence: 99%

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Vrana

Lavalle²,

Dokmeci³

et al. 2013

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

show abstract

“…Development of these micro engineering approaches has opened entirely new possibilities to create in vitro models that reconstitute more complex 3D organ level structures and to integrate crucial dynamic mechanical cues as well as chemical signals. There have been different kinds of tissue or organ chip models for specific research (Figure 6), such as liver-on-a-chip, [96] kidney-on-a-chip, [97,98] gut-on-a-chip, [99,100] lung-on-a-chip, [101,102] heart-on-a-chip [103] and vessel-on-a-chip [104].…”

Section: Microfluidic Applications In Cell Biologymentioning

confidence: 99%

Development and Applications of Microfluidic Devices for Cell Culture in Cell Biology

Yi¹,

Lin²

2016

Mol Biol

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Development of microfluidic culture technology combined with tissue engineering catalyzes the progress in study of cell biology. These tools will promote the understanding of physiological and pathological changes. Cancer cells and stem cells are sensitive to their surroundings, thus could be better explored by controllable microfluidic devices. In this review, we describe the ways to control cell microenvironment and explain how the influencing factors influence cellular behaviors, then present microfluidic-chip-based exemplary applications for cancer models and stem cell differentiation.

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“…In this context, many studies have focused on generating organ-on-a-chip models of the liver and kidney due to the importance of theses organs in drug metabolism and clearance [13][14][15]. Models of other important organs, e.g., lung [16,17], skin [18][19][20], and the gastrointestinal (GI) tract [21,22], have also been developed for toxicity testing, since those organs are closely related to allergic and immunogenic disorders caused by exogenous substances [23,24].…”

Section: Organ-on-a-chip: Towards Improved Toxicity Assessmentmentioning

confidence: 99%

Organ-On-A-Chip: Development and Clinical Prospects Toward Toxicity Assessment with an Emphasis on Bone Marrow

et al. 2015

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Conventional approaches for toxicity evaluation of drugs and chemicals, such as animal tests, can be impractical due to the large experimental scale and the immunological differences between species. Organ-on-a-chip models have recently been recognized as a prominent alternative to conventional toxicity tests aiming to simulate the human in vivo physiology. This review focuses on the organ-on-a-chip applications for high-throughput screening of candidate drugs against toxicity, with a particular emphasis on bone-marrow-on-a-chip. Studies in which organ-on-a-chip models have been developed and utilized to maximize the efficiency and predictability in toxicity assessment are introduced. The potential of these devices to replace tests of acute systemic toxicity in animals, and the challenges that are inherent in simulating the human immune system are also discussed. As a promising approach to overcome the limitations, we further focus on an in-depth analysis of the development of bone-marrow-on-a-chip that is capable of simulating human immune responses against external stimuli due to the key roles of marrow in immune systems with hematopoietic activities. Owing to the complex interactions between hematopoietic stem cells and marrow microenvironments, precise control of both biochemical and physical niches that are critical in maintenance of hematopoiesis remains a key challenge. Thus, recently developed bone-marrow-on-a-chip models support immunogenicity and immunotoxicity testing in long-term cultivation with repeated antigen stimulation. In this review, we provide an overview of clinical studies that have been carried out on bone marrow transplants in patients with immune-related diseases and future aspects of clinical and pharmaceutical application of bone-marrow-on-a-chip.

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On chip porous polymer membranes for integration of gastrointestinal tract epithelium with microfluidic ‘body-on-a-chip’ devices

Cited by 161 publications

References 29 publications

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Development and Applications of Microfluidic Devices for Cell Culture in Cell Biology

Organ-On-A-Chip: Development and Clinical Prospects Toward Toxicity Assessment with an Emphasis on Bone Marrow

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