On-chip selection of adenosine aptamer using graphene oxide-coated magnetic nanoparticles

Lim, Hosub; Chang, Junhyuck; Kim, Kyung‐Il; Moon, Youngkwang; Lee, Saebom; Lee, Byoungsang; Lee, Jung Heon; Lee, Jinkee

doi:10.1063/5.0095419

Cited by 7 publications

(3 citation statements)

References 49 publications

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Section: Comparison Of Sensing Response Of Cnt and Graphene Fet Aptas...contrasting

confidence: 57%

“…Recent work from literature using graphene oxide achieved a dissociation constant for an adenosine aptasensor (52-mer) of 18.6 ± 1.5 µM in eight cycles of aptamer selection from isothermal titration calorimetry regardless of its conformational change [241]. The results from this study are significantly lower compared to the previous research published in the literature for adenosine detection [221,222,241]. Hill coefficient value of n < 1 indicates negative cooperative for both CNT and graphene, which is consistent with literature using aptasensors on graphene FET [218,219], CNT FETs [220] and our work on CNT FETs using the same 35-mer aptamer discussed in Chapter 5.…”

Section: Comparison Of Sensing Response Of Cnt and Graphene Fet Aptas...mentioning

confidence: 99%

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Carbon nanomaterials as platforms for biosensors and atomic switch network devices

Nguyen

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Carbon nanomaterials, such as carbon nanotubes (CNTs) and graphene, have attracted growing attention as sensing platforms for field effect transistor (FET) aptasensors due to their large surface area and real-time detection capability. Moreover, carbon nanotubes offer high levels of sensitivity for various analytes detection and use as network material to increase the stability in atomic switch network (ASN) devices. This thesis investigated the detection of small molecules using different carbon nanomaterial platforms, as well as the use of CNT networks for atomic switch networks. First, CNT FET aptasensors have been investigated for the detection of adenosine using two different aptamer sequences, a 35-mer and a 27-mer. Limits of detection were found for adenosine of 100 pM and 320 nM for the 35-mer and 27-mer aptamers, with dissociation constants of 1.2 nM and 160 nM, respectively. Upon analyte recognition, the 35-mer adenosine aptamer adopts a compact G-quadruplex structure, while the 27-mer adenosine aptamer changes to a folded duplex. Using the CNT FET aptasensor platform, adenosine could be detected with high sensitivity over the range of 100 pM to 10 µM, highlighting the suitability of the CNT FET aptasensor platform for high-performance adenosine detection. The aptamer restructuring format is critical for high sensitivity with the G-quadruplex aptasensor having a 130-fold smaller dissociation constant than the duplex forming aptasensor. Second, a comparison between CNT and graphene FET aptasensors has been conducted. CNT and graphene both showed high sensitivity over the detection range of 1 nM to 10 mM, with dissociation constants of 7.7 nM for CNT and 5.7 nM for graphene. Compared to graphene FET aptasensors, CNT FET showed eight times higher sensitivity. CNT FETs have also performed for the study of dose-dependent sensing to evaluate the feasibility of realizing accurate biosensors. Regardless of the initial ligand concentration, the real-time current responses showed a similar signal on the first added concentration. These sensing signals could be caused by CNT platforms with sensing hotspots between CNT junctions, or by over-detecting sensing sites, rather than accurately responding to analyte levels. Overall, the sensing performance of CNT FET aptasensors is influenced by the initial concentrations, with the sensitivity increasing for lower initial concentrations near the detection limit. Finally, distribution of silver nanowires (AgNWs) in the ASN device results in Joule heating at nanowire contact points, resulting in failure of AgNWs in the network. To solve this issue, a combination of CNTs has been used to improve the electrical conductivity of ASN devices. A simple fabrication and measurement method for ASN-based CNT devices has been developed. This technique involves drop-casting silver nanowires onto a patterned carbon nanotube network and then exposing it to sulfur gas to activate the nanowires. Because of the combination of AgNWs and carbon nanotubes, the ASN system improved contact resistance and switching events. This work is the first to introduce an Ag/Ag2S/Ag carbon nanotube that can be potentially applied to neural computing applications. Plans for future work are presented at the end of this thesis.

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Section: Comparison Of Sensing Response Of Cnt and Graphene Fet Aptas...contrasting

confidence: 57%

Section: Comparison Of Sensing Response Of Cnt and Graphene Fet Aptas...mentioning

confidence: 99%

Carbon nanomaterials as platforms for biosensors and atomic switch network devices

Nguyen

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“…Recently, the magnetic particles that modified graphene oxide (GO) (MGO)-based SELEX have garnered substantial attention owing to its simplified separation procedure compared to conventional GO-SELEX [ 30 , 31 ]. Moreover, this nonimmobilized selection approach preserves the natural binding states of the targets and aptamer candidates, thereby enhancing its reliability in practical applications.…”

Section: Introductionmentioning

confidence: 99%

Single-Round Circular Aptamer Discovery Using Bioinspired Magnetosome-Like Magnetic Chain Cross-Linked Graphene Oxide

Yao,

Feng,

Zhou

et al. 2024

Research

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Circular aptamers are promising candidates for analytical and therapeutic applications due to their enhanced biological and structural stability. However, the process of circular aptamer selection remains a great challenge, as it requires multiple rounds of binding–separation–amplification that involves issues with nonspecific binding and amplification bias. Here, we develop a highly practical solution for reliable selection of circular aptamers in a single round based on magnetosome-like magnetic chain cross-linked graphene oxide (separation efficiency ≈ 10 5 ). High-affinity aptamer candidates can be rapidly selected from a preenriched circular DNA library, while low-affinity candidates are effectively adsorbed and separated by magnetosome-like magnetic chain cross-linked graphene oxide. With lipopolysaccharide as a representative model, the single-round selected lipopolysaccharide circular aptamer has been identified to have a high binding affinity with a K d value of low to nanomolar range. Using this method, circular aptamers for protein and small-molecule targets were also successfully generated. We envision that this approach will accelerate the discovery of various new circular aptamers and open up a new avenue for analytical and therapeutic studies.

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DNA-Wrapped CNT Sensor for Small Nucleic Acid Detection: Influence of Short Complementary Sequence

et al. 2022

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On-chip selection of adenosine aptamer using graphene oxide-coated magnetic nanoparticles

Cited by 7 publications

References 49 publications

Carbon nanomaterials as platforms for biosensors and atomic switch network devices

Carbon nanomaterials as platforms for biosensors and atomic switch network devices

Single-Round Circular Aptamer Discovery Using Bioinspired Magnetosome-Like Magnetic Chain Cross-Linked Graphene Oxide

DNA-Wrapped CNT Sensor for Small Nucleic Acid Detection: Influence of Short Complementary Sequence

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