On-chip stool liquefaction <i>via</i> acoustofluidics

Zhao, Shuaiguo; He, Weihua; Ma, Zhehan; Liu, Peiyao; Huang, Po‐Hsun; Bachman, Hunter; Wang, Lin; Yang, Shujie; Tian, Zhenhua; Wang, Zeyu; Gu, Yuyang; Xie, Zhemiao; Huang, Tony Jun

doi:10.1039/c8lc01310a

Cited by 44 publications

(35 citation statements)

References 59 publications

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“…Acoustofluidics, the field of microscale fluid manipulation using acoustics, has utility in many applications, including drug delivery, microorganism manipulation, analyte mixing, and lab‐on‐a‐chip fluid pumping . In a typical acoustofluidic system, either Rayleigh surface acoustic waves (SAWs) or bulk waves traveling on the surface or in the bulk of a microfluidic device, respectively, is the dominant wave propagation mode.…”

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confidence: 99%

“…In a typical acoustofluidic system, either Rayleigh surface acoustic waves (SAWs) or bulk waves traveling on the surface or in the bulk of a microfluidic device, respectively, is the dominant wave propagation mode. Such an acoustic actuation mechanism can force the microchannel walls, microbubbles, or sharp‐edge elastic geometries embedded inside the microfluidic device to oscillate. A result of this oscillation is a nonperiodic fluid motion generated near the oscillating object in its surrounding liquid.…”

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confidence: 99%

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Dancing with the Cells: Acoustic Microflows Generated by Oscillating Cells

Salari

Appak-Baskoy

Ezzo

et al. 2019

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The interaction of a sound or ultrasound wave with an elastic object, such as a microbubble, can give rise to a steady‐state microstreaming flow in its surrounding liquid. Many microfluidic strategies for cell and particle manipulation, and analyte mixing, are based on this type of flow. In addition, there are reports that acoustic streaming can be generated in biological systems, for instance, in a mammalian inner ear. Here, new observations are reported that individual cells are able to induce microstreaming flow, when they are excited by controlled acoustic waves in vitro. Single adherent cells are exposed to an acoustic field inside a microfluidic device. The cell‐induced microstreaming is then investigated by monitoring flow tracers around the cell, while the structure and extracellular environment of the cell are altered using different chemicals. The observations suggest that the maximum streaming flow induced by an MDA‐MB‐231 breast cancer cell can reach velocities on the order of mm s−1, and this maximum velocity is primarily governed by the overall cell stiffness. Therefore, such cell‐induced microstreaming measurements, including flow pattern and velocity magnitude, may be used as label‐free proxies of cellular mechanical properties, such as stiffness.

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confidence: 99%

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confidence: 99%

Dancing with the Cells: Acoustic Microflows Generated by Oscillating Cells

Salari

Appak-Baskoy

Ezzo

et al. 2019

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“…SAW-II, where an external flow in microchannels enables a flow-guided patterned segregation of different target agents . Lastly, we discuss acoustofluidic devices called streaming-driven tweezers, where acoustically driven flow enables both fluid manipulation and transport of agents trapped in streaming-induced fluid oscillations [77][78][79][80][81][82][83][84][85][86][87][88][89][90][91].…”

Section: Passive Agents and Actuation Strategiesmentioning

confidence: 99%

Contactless acoustic micro/nano manipulation: a paradigm for next generation applications in life sciences

2020

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Acoustic actuation techniques offer a promising tool for contactless manipulation of both synthetic and biological micro/nano agents that encompass different length scales. The traditional usage of sound waves has steadily progressed from mid-air manipulation of salt grains to sophisticated techniques that employ nanoparticle flow in microfluidic networks. State-of-the-art in microfabrication and instrumentation have further expanded the outreach of these actuation techniques to autonomous propulsion of micro-agents. In this review article, we provide a universal perspective of the known acoustic micromanipulation technologies in terms of their applications and governing physics. Hereby, we survey these technologies and classify them with regards to passive and active manipulation of agents. These manipulation methods account for both intelligent devices adept at dexterous non-contact handling of micro-agents, and acoustically induced mechanisms for self-propulsion of micro-robots. Moreover, owing to the clinical compliance of ultrasound, we provide future considerations of acoustic manipulation techniques to be fruitfully employed in biological applications that range from label-free drug testing to minimally invasive clinical interventions.

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“…One challenge with detecting pathogens or microbiome disturbances from clinical and field samples lies in the required pre-processing of the sample. In the case of detection from stool samples, for example, processing techniques are laborious and time-consuming, requiring highly trained personnel and advanced equipment (Zhao et al, 2019). Towards the goal of making this process portable and more efficient, Zhao et al created an on-chip microfluidic stool liquefaction device using acoustofluidics, which both filters out large debris and homogenizes the sample without damaging bacteria that could be analysed in a later step (Zhao et al, 2019).…”

Section: Advan Ce S and Challeng E S With De Tec Ti On From Clini Cmentioning

confidence: 99%

“…In the case of detection from stool samples, for example, processing techniques are laborious and time-consuming, requiring highly trained personnel and advanced equipment (Zhao et al, 2019). Towards the goal of making this process portable and more efficient, Zhao et al created an on-chip microfluidic stool liquefaction device using acoustofluidics, which both filters out large debris and homogenizes the sample without damaging bacteria that could be analysed in a later step (Zhao et al, 2019). Studies such as this one suggest that fully functional portable biosensors for direct use with clinical samples are realizable, although for the most part,…”

Section: Advan Ce S and Challeng E S With De Tec Ti On From Clini Cmentioning

confidence: 99%

The future of microbiome analysis: Biosensor methods for big data collection and clinical diagnostics

Akarapipad

Yoon

2020

Med Devices & Sens

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Worldwide interest in the human microbiome is rapidly expanding, as the microbiome is a potential key to understanding human health and improving diagnostic and treatment strategies. A wide array of microorganisms, including bacteria, archaea, unicellular/multicellular eukaryotes (i.e. fungi and helminths) and viruses, colonize on the human body. Their activity and interactions with each other impact their host (Rowan-Nash, Korry, Mylonakis, & Belenky, 2019). Although sometimes used interchangeably, the word 'microbiota' refers to the collective community of microorganisms residing in a particular environment (such as a region of the body), whereas the word 'microbiome' refers to the entirety of this community's genetic information (Allaband et al., 2019; Kuczynski et al., 2012). The different regions of the human body contain various combinations of microbes that could provide information not only about infectious diseases at specific sites, but also about the potential health conditions of a person (Mimee et al., 2018). It is remarkable to consider that, according to recently revised estimates, the human body houses approximately as many bacteria (the most abundant member of the microbiota) as human cells, with bacteria comprising 0.3% of total body mass (Sender, Fuchs, & Milo, 2016). According to the

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On-chip stool liquefaction via acoustofluidics

Abstract: An acoustofluidic chip can liquefy stool samples in a continuous flow.

Cited by 44 publications

References 59 publications

Dancing with the Cells: Acoustic Microflows Generated by Oscillating Cells

Dancing with the Cells: Acoustic Microflows Generated by Oscillating Cells

Contactless acoustic micro/nano manipulation: a paradigm for next generation applications in life sciences

The future of microbiome analysis: Biosensor methods for big data collection and clinical diagnostics

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