On-column radiometric detector for capillary isotachophoresis

Kaniansky, Dušan; Rajec, P.; Švec, A.; Marák, Jozef; Koval, M.; Lúčka, M.; Franko, Š.; Sabanoš, G.

doi:10.1007/bf02039828

Cited by 16 publications

(7 citation statements)

References 22 publications

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“…Radioisotope detection of 32P, 14C, and "Tc has previously been reported by Kaniansky et al for isotachophoresis (7,20).…”

mentioning

confidence: 67%

“…Equation 2suggests that the number of counts measured (the detector sensitivity) over a sample peak may be increased by lengthening the residence time of the sample in the detection volume. This is equivalent to increasing the counting time on a liquid scintillation counter and this concept has been recognized in both radio HPLC applications (see, for example, ref 15) and radioisotope detection in isotachophoresis (7,20). In capillary electrophoresis, the velocity of a sample zone may be reduced and its residence time increased by simply reducing the applied potential as the zone passes through the detection volume.…”

Section: Resultsmentioning

confidence: 99%

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On-line radioisotope detection for capillary electrophoresis

Pentoney¹,

Zare²,

Quint³

1989

Anal. Chem.

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“…Radioisotope detection of 32P, 14C, and "Tc has previously been reported by Kaniansky et al for isotachophoresis (7,20).…”

mentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

On-line radioisotope detection for capillary electrophoresis

Pentoney¹,

Zare²,

Quint³

1989

Anal. Chem.

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“…When a neuron is grown in an 3'S-methionine rich culturing media, the newly synthesized methionine-containing peptides will be radiolabeled. While several radionuclide detection systems have been described for CE [ 18,191 and isotachophoresis [20,21], these implementations do not work well for 35S because the beta particles lack sufficient energy to penetrate the capillary wall and reach the detector. Consequently, previous approaches lack the required sensitivity.…”

Section: Radionuclide Detectionmentioning

confidence: 98%

Novel detection schemes for the trace analysis of amino acids and peptides using capillary electrophoresis

Sweedler

Fuller

Timperman

et al. 1993

J Microcolumn Separations

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Abstract. The ability of the cellular biochemist to understand events occurring in small subsections of an individual cell depends on available analytical techniques. Two unique capillary electrophoresis (CE) detection schemes for trace level peptide analysis are characterized: an on-line, multichannel, laser-induced fluorescence system and a postcolumn radionuclide system. The fluorescence system has picomolar concentration detection limits (and zeptomole mass limits of detection) for tagged amino acids and peptides. Preliminary results are presented for a method to enhance the ability to fluorescently tag low concentrations of amino acids and peptides from neuronal subsections. The procedure takes advantage of the high local concentration of peptides within a synaptic vesicle to enhance the derivatization reaction. The other detection method described involves a post-column 35S detector to examine methionine or cysteine containing peptides. This system deposits the effluent from the separation capillary onto a membrane treated with a solid scintillator. After drying the membrane, the photon emission is imaged with a solid-state integrating detector. The sensitivity for 35S labeled components is in the attomole range.

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“…While there are numerous examples of MCE employed for pharmaceutical analysis [ 73 , 76 ], there have been very few examples of CE methods, and no examples of MCE methods, applied to the analysis of radiopharmaceuticals. Separation and detection of 99m Tc-labeled SPECT compounds has been performed with capillary zone electrophoresis and isotachophoresis [ 77 , 78 , 79 , 80 , 81 ], and use of CE has been suggested for analysis of PET tracers in a patent application [ 30 ]. Recently, using the PET tracers [ 18 F]FLT and [ 18 F]FAC as model systems, our group explored the feasibility of using micellar electrokinetic chromatography (MEKC) to separate neutral tracers from neutral impurities, and showed comparable separation and limit of detection as HPLC/UV [ 82 ].…”

Section: Miniaturization Of Quality Control Testsmentioning

confidence: 99%

Recent Progress toward Microfluidic Quality Control Testing of Radiopharmaceuticals

Sadeghi

Dam

2017

Micromachines

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Radiopharmaceuticals labeled with short-lived positron-emitting or gamma-emitting isotopes are injected into patients just prior to performing positron emission tomography (PET) or single photon emission tomography (SPECT) scans, respectively. These imaging modalities are widely used in clinical care, as well as in the development and evaluation of new therapies in clinical research. Prior to injection, these radiopharmaceuticals (tracers) must undergo quality control (QC) testing to ensure product purity, identity, and safety for human use. Quality tests can be broadly categorized as (i) pharmaceutical tests, needed to ensure molecular identity, physiological compatibility and that no microbiological, pyrogenic, chemical, or particulate contamination is present in the final preparation; and (ii) radioactive tests, needed to ensure proper dosing and that there are no radiochemical and radionuclidic impurities that could interfere with the biodistribution or imaging. Performing the required QC tests is cumbersome and time-consuming, and requires an array of expensive analytical chemistry equipment and significant dedicated lab space. Calibrations, day of use tests, and documentation create an additional burden. Furthermore, in contrast to ordinary pharmaceuticals, each batch of short-lived radiopharmaceuticals must be manufactured and tested within a short period of time to avoid significant losses due to radioactive decay. To meet these challenges, several efforts are underway to develop integrated QC testing instruments that automatically perform and document all of the required tests. More recently, microfluidic quality control systems have been gaining increasing attention due to vastly reduced sample and reagent consumption, shorter analysis times, higher detection sensitivity, increased multiplexing, and reduced instrumentation size. In this review, we describe each of the required QC tests and conventional testing methods, followed by a discussion of efforts to directly miniaturize the test or examples in the literature that could be implemented for miniaturized QC testing.

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On-column radiometric detector for capillary isotachophoresis

Cited by 16 publications

References 22 publications

On-line radioisotope detection for capillary electrophoresis

On-line radioisotope detection for capillary electrophoresis

Novel detection schemes for the trace analysis of amino acids and peptides using capillary electrophoresis

Recent Progress toward Microfluidic Quality Control Testing of Radiopharmaceuticals

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