On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity

Gao, Feng; Djordjević, Ivan; Pokholenko, Oleksandr; Zhang, Haobo; Zhang, Junying; Steele, Terry W. J.

doi:10.3390/molecules23040796

Cited by 24 publications

(21 citation statements)

References 47 publications

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“…Experiments show that the surface functional groups of PAMAM dendrimers increase exponentially with the next generation and the concentration of -NH2 groups may be related to their toxicity (31). The order of cytotoxicity is hydroxyl-terminated < carboxyl-terminated < aminoterminated PAMAM dendrimers (32).…”

Section: Discussionmentioning

confidence: 99%

Remineralization of dentin induced by a compound of polyamide-amine and chlorhexidine in a resin dentin bonding microenvironment

Xiang

Chen

et al. 2021

Ann Transl Med

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Background: The purpose of this study was to investigate the effect of a complex of polyamide-amine dendrimer (PAMAM) and chlorhexidine gluconate (CG) on remineralization of dentin in an artificial simulated resin dentin bonding microenvironment.Methods: The structure of this complex was characterized by FT-IR. Twelve standard dentin samples were randomly divided into four treatment fluid groups namely a PAMAM group, CG group, PAMAM + CG group, and deionized water group. A microenvironmental mineralization model was established in vitro with 50 μm gap width between resin and dentin. The dentin surface was observed by a scanning electron microscope (SEM), and the chemical structure of the surface was analyzed by X-ray energy spectrum (EDS), X-ray diffraction (XRD), and laser Raman spectroscopy.Results: SEM showed the density of dentinal tubules exposed in the PAMAM group decreased after a 14-day immersion, with corn rod-shaped crystal structures gathered around the tubules. In addition, visible mineralization occurred in partial areas of the CG group, rod-shaped crystals and in comparison, dentinal tubules in the PAMAM + CG group were almost completely covered by flaky crystal structures. Raman spectrum analysis showed that crystals formed by PAMAM, CG, and PAMAM + CG solution all had strong phosphate characteristic peaks, indicating the presence of hydroxyapatite (HA), that of the PAMAM + CG group was the strongest. The EDS results showed that the Ca and P levels of the PAMAM group and the CG group were slightly higher than those of the deionized water group, while PAMAM + CG group significantly higher than the others, Ca/P value approaching 1.67. The results of XRD showed the characteristic peaks of hydroxyapatite detected by the PAMAM + CG group at 2θ=26.0 (002), 2θ=32.0 (211), and 2θ=33.0 (112) were high and sharp, with a few diffraction line burrs indicating it had high crystallinity and purity. The Scherrer equation results showed that the appearance and size of the grains formed by the PAMAM + CG group were basically consistent with those of healthy dentin.Conclusions: Altogether, the compound of polyamide-amine dendrimer and chlorhexidine could induce the remineralization of human dentin in a resin dentin bonding microenvironment with a gap of 50 μm to form a crystal structure similar to dentin hydroxyapatite.

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Section: Discussionmentioning

confidence: 99%

Remineralization of dentin induced by a compound of polyamide-amine and chlorhexidine in a resin dentin bonding microenvironment

Xiang

Chen

et al. 2021

Ann Transl Med

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“…Gao F. et al (2018) synthesized UV light-responsive PAMAM dendrimer for tissue adhesive by conjugating 4-(3-(trifluoromethyl)-3H-diazirin-3-yl) benzyl bromide onto amine groups on the peripheral of dendrimer (PAMAM-g-diazirine) [87].…”

Section: Recent Trends Of Stimuli-responsive Dendrimers In Controlmentioning

confidence: 99%

Recent Progress and Advances of Multi-Stimuli-Responsive Dendrimers in Drug Delivery for Cancer Treatment

Nguyen

Cao

et al. 2019

Pharmaceutics

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Despite the fact that nanocarriers as drug delivery systems overcome the limitation of chemotherapy, the leakage of encapsulated drugs during the delivery process to the target site can still cause toxic effects to healthy cells in other tissues and organs in the body. Controlling drug release at the target site, responding to stimuli that originated from internal changes within the body, as well as stimuli manipulated by external sources has recently received significant attention. Owning to the spherical shape and porous structure, dendrimer is utilized as a material for drug delivery. Moreover, the surface region of dendrimer has various moieties facilitating the surface functionalization to develop the desired material. Therefore, multi-stimuli-responsive dendrimers or ‘smart’ dendrimers that respond to more than two stimuli will be an inspired attempt to achieve the site-specific release and reduce as much as possible the side effects of the drug. The aim of this review was to delve much deeper into the recent progress of multi-stimuli-responsive dendrimers in the delivery of anticancer drugs in addition to the major potential challenges.

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“…Previous studies have also focused on biocompatibility and cytotoxicity behavior of the primary components. Subcutaneous implants of PDz without PEG display a moderate immunological response, which can be tuned by removing the cationic charge or through electrostatic charge pairing …”

Section: Comparison Of Energy Generated Per Pulse Versus Required Actmentioning

confidence: 99%

“…[12] Previous studies have also focused on biocompatibility and cytotoxicity behavior of the primary components. Subcutaneous implants of PDz without PEG display a moderate immunological response, which can be tuned by removing the cationic charge [18] or through electrostatic charge pairing. [10] Utilizing PAMAM's miscibility with PEG 400 liquid polymers, non-aqueous adhesive blends offer faster gelation times and low viscosity formulations that satisfy syringe-adaptable criteria.…”

Section: Light-activated Tissue Adhesives Are Limited To Low Light Domentioning

confidence: 99%

Pulsed Laser Activation of Carbene Bioadhesive Boosts Bonding Strength

Shah

Kartheek

Gandhi

et al. 2019

Macro Materials & Eng

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Light‐activated tissue adhesives are limited to low light doses (50 J) and intensities (<1 W cm−2) due to photo‐to‐thermal heat generation. Low intensities have the disadvantage of limited penetration depths with retarded crosslinking kinetics, which impairs carbene‐based crosslinking strategies that compete with nitrogen evolution and gas nucleation. These limitations are circumvented by a trade‐off between high‐intensity activation while reducing the exposure surface area. Continuous or pulsed activation by line scanning the carbene precursor adhesive allows curing a higher surface area/volume ratio while preventing localized heat generation. By optimizing irradiation with a pulsed laser scan, the adhesion strength is improved by 17‐fold over ultraviolet A (UVA) light emitting diodes (LEDs) and is on par with bioadhesive gold standard of topical cyanoacrylates. Overall, this improved method of photo‐activation applies to other industrial and clinical photocuring adhesives where limits on UVA dose constrain exposure intensities.

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On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity

Cited by 24 publications

References 47 publications

Remineralization of dentin induced by a compound of polyamide-amine and chlorhexidine in a resin dentin bonding microenvironment

Remineralization of dentin induced by a compound of polyamide-amine and chlorhexidine in a resin dentin bonding microenvironment

Recent Progress and Advances of Multi-Stimuli-Responsive Dendrimers in Drug Delivery for Cancer Treatment

Pulsed Laser Activation of Carbene Bioadhesive Boosts Bonding Strength

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