On-Demand Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes

Huang, Jian‐An; Caprettini, Valeria; Zhao, Yingqi; Melle, Giovanni; Maccaferri, Nicolò; Deleye, Lieselot; Zambrana‐Puyalto, Xavier; Ardini, Matteo; Tantussi, Francesco; Dipalo, Michele; Angelis, Francesco De

doi:10.1021/acs.nanolett.8b03764

Cited by 65 publications

(64 citation statements)

References 75 publications

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“…In a report from the same group, Huang et al used gold‐coated nanostraws, coupled with electroporation to deliver Raman‐tagged gold nanorods into fibroblast cells (NIH‐3T3) with single‐particle precision ( Figure ) . Surface‐enhanced Raman scattering from the gold nanorods, plus shielding from the delivery reservoir by the gold‐coated nanostraw layer, means single nanorods could be visualized as a momentary increases in the Raman signal as they passed through the nanostraw.…”

Section: Biochemical Sensingmentioning

confidence: 99%

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High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

et al. 2020

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Materials patterned with high‐aspect‐ratio nanostructures have features on similar length scales to cellular components. These surfaces are an extreme topography on the cellular level and have become useful tools for perturbing and sensing the cellular environment. Motivation comes from the ability of high‐aspect‐ratio nanostructures to deliver cargoes into cells and tissues, access the intracellular environment, and control cell behavior. These structures directly perturb cells' ability to sense and respond to external forces, influencing cell fate, and enabling new mechanistic studies. Through careful design of their nanoscale structure, these systems act as biological metamaterials, eliciting unusual biological responses. While predominantly used to interface eukaryotic cells, there is growing interest in nonanimal and prokaryotic cell interfacing. Both experimental and theoretical studies have attempted to develop a mechanistic understanding for the observed behaviors, predominantly focusing on the cell–nanostructure interface. This review considers how high‐aspect‐ratio nanostructured surfaces are used to both stimulate and sense biological systems.

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Section: Biochemical Sensingmentioning

confidence: 99%

“…Raman correlation spectroscopy was used to track surface‐enhanced Raman scattering from single gold nanorods passing through the nanostraws. Reproduced with permission . Copyright 2019, American Chemical Society.…”

Section: Biochemical Sensingmentioning

confidence: 99%

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

et al. 2020

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“…The microfluidic embedded platform performs as nanoelectrodes for cell electroporation, nanochannels for electrophoretic delivery, as well as plasmonic antennae. [ 17 ]…”

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confidence: 99%

Silicon‐Nanotube‐Mediated Intracellular Delivery Enables Ex Vivo Gene Editing

Chen

Aslanoglou

Murayama³

et al. 2020

Advanced Materials

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Engineered nano–bio cellular interfaces driven by vertical nanostructured materials are set to spur transformative progress in modulating cellular processes and interrogations. In particular, the intracellular delivery—a core concept in fundamental and translational biomedical research—holds great promise for developing novel cell therapies based on gene modification. This study demonstrates the development of a mechanotransfection platform comprising vertically aligned silicon nanotube (VA‐SiNT) arrays for ex vivo gene editing. The internal hollow structure of SiNTs allows effective loading of various biomolecule cargoes; and SiNTs mediate delivery of those cargoes into GPE86 mouse embryonic fibroblasts without compromising their viability. Focused ion beam scanning electron microscopy (FIB‐SEM) and confocal microscopy results demonstrate localized membrane invaginations and accumulation of caveolin‐1 at the cell–NT interface, suggesting the presence of endocytic pits. Small‐molecule inhibition of endocytosis suggests that active endocytic process plays a role in the intracellular delivery of cargo from SiNTs. SiNT‐mediated siRNA intracellular delivery shows the capacity to reduce expression levels of F‐actin binding protein (Triobp) and alter the cellular morphology of GPE86. Finally, the successful delivery of Cas9 ribonucleoprotein (RNP) to specifically target mouse Hprt gene is achieved. This NT‐enhanced molecular delivery platform has strong potential to support gene editing technologies.

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“…With this strategy, Messina et al reported a hollow plasmonic gold nanotube‐based platform for delivery of a wide variety of cargo into the cell interior with prominent advantages such as single‐cell resolution and the potential of multiplexed applications (Figure 10b). [ 51 ] Moreover, single nanoparticles could also be delivered into single cells of choice using these optoporation‐enhanced hollow gold nanotubes, [ 169 ] thus achieving a new level of delivery precision.…”

Section: Assisted Penetrationmentioning

confidence: 99%

Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane

He¹,

Hu²,

et al. 2020

Adv Funct Materials

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Establishing techniques to efficiently and nondestructively access the intracellular milieu is essential for many biomedical and scientific applications, ranging from drug delivery, to electrical recording, to biochemical detection. Cell penetration using nanoneedle arrays is currently a research focus area because it not only meets the increasing therapeutic demands of cell modifications and genome editing, but also provides an ideal platform for tracking long-term intracellular information. Although the precise mechanism driving membrane penetration by nanoneedle arrays is still unclear, the low cytotoxicity, wide range of delivered materials, diverse cell type targets, and simple material structures of nanoneedle arrays make these splendid platforms for cell access. Here, the recent progress in this field is reviewed by examining device architectures and discussing mechanisms for nanoneedle penetration, and the major studies demonstrating the most general applicability of nanoneedle arrays, typical methodologies to access the intracellular environment using nanoneedles with spontaneous or assisted penetration modes, as well as biosafety aspects are presented. This review should be valuable for deeply understanding the materials fabrication principles, device designs, cell penetration methodologies, biosafety aspects, and application strategies of nanoneedle array-based systems that are of crucial importance for the development of future practical biomedical platforms.

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On-Demand Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes

Cited by 65 publications

References 75 publications

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

Silicon‐Nanotube‐Mediated Intracellular Delivery Enables Ex Vivo Gene Editing

Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane

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