On designing stable magnetic vectors as carriers for malaria DNA vaccine

Al-Deen, Fatin Muhammed Nawwab; Selomulya, Cordelia; Williams, Tim

doi:10.1016/j.colsurfb.2012.09.026

Cited by 25 publications

(22 citation statements)

References 47 publications

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“…Following mTBI the pattern and extent of neurodegeneration observed in these experiments were in accordance with the those reported previously [9]. mTBI causes neurodegeneration and the majority of FJ-positive cells were found within the cerebral cortex (Figure 3B), hippocampus (Figure 3C) and thalamus (data not shown).…”

Section: Resultssupporting

confidence: 91%

“…These low cost materials are biocompatible and can easily be surfacemodified with a variety of peptides, antibodies or small molecules for targeting and to increase their transfection efficiency. Moreover, it has been shown that ‘magnetofection’ the use of a magnet to draw magnetic nanoparticles to a specific area, can efficiently increase the speed and efficiency of the gene transfection in vitro and site specific targeting in vivo [9, 10]. …”

Section: Introductionmentioning

confidence: 99%

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Magnetic micelles for DNA delivery to rat brains after mild traumatic brain injury

Das

Wang

Bedi

et al. 2014

Nanomedicine: Nanotechnology, Biology and Medicine

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Traumatic brain injury (TBI) causes significant mortality, long term disability and psychological symptoms. Gene therapy is a promising approach for treatment of different pathological conditions. Here we tested chitosan and polyethyleneimine (PEI)-coated magnetic micelles (CPmag micelles or CPMMs), a potential MRI contrast agent, to deliver a reporter DNA to the brain after mild TBI (mTBI). CPMM - tomato plasmid (ptd) conjugate expressing a red-fluorescent protein (RFP) was administered intranasally immediately after mTBI or sham surgery in male SD rats. Evans blue extravasation following mTBI suggested CPMM-ptd entry into the brain via the compromised blood-brain barrier. Magnetofection increased the concentration of CPMMs in the brain. RFP expression was observed in the brain (cortex and hippocampus), lung and liver 48 hours after mTBI. CPMM did not evoke any inflammatory response by themselves and were excreted from the body. These results indicate the possibility of using intranasally administered CPMM as a theranostic vehicle for mTBI.

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Magnetic micelles for DNA delivery to rat brains after mild traumatic brain injury

Das

Wang

Bedi

et al. 2014

Nanomedicine: Nanotechnology, Biology and Medicine

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“…DNAse enzyme is responsible for degradation of pDNA and is the major obstacle for pDNA transfection in the cells. Our results are in concordance with previous reports which proved that complexation of pDNA with polymeric or lipidic delivery system protect pDNA from DNAse enzyme and enhance its stability in biological milieu (Jinturkar et al, 2012;Al-Deen et al, 2013). The polymethacrylate vectors could protect pDNA from DNAse enzyme very effectively which will improve in vivo transfection.…”

Section: Dnase I Sensitivity Testsupporting

confidence: 92%

Microbeads mediated oral plasmid DNA delivery using polymethacrylate vectors: an effectual groundwork for colorectal cancer

Bhatt¹,

Khatri²,

Kumar³

et al. 2014

Drug Delivery

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This study was aimed to develop and evaluate p53 polyplex-loaded enteric-coated calcium pectinate microbeads for oral gene delivery as an effective novel alternative for colorectal cancer therapy. Mutation in p53 is the key event in colorectal cancer (CRC) and an important target for the treatment of CRC through gene therapy. Polymethacrylates-based non-viral vectors were evaluated for their ability to complex, protect and transfect p53 (wt) into colon cancer cell line. Polyplexes were formulated by complexation of cationic polymer with anionic pDNA at different N/P ratios. p53 polyplex-loaded calcium pectinate (CP) microbeads were prepared by ionotropic gelation of pectin with calcium chloride and coated with Eudragit Õ S100. In vitro release studies showed that enteric-coated CP microbeads protected the release of p53 polyplex in upper GIT with less than 10% release. In-vitro cell line studies and in vivo studies in rat showed that polymethacrylate carrier could transfect the pDNA effectively. Results of in vivo gene expression study further confirmed the ability of enteric-coated calcium pectinate microbeads to deliver pDNA specifically to rat colon. Conclusively, enteric-coated calcium pectinate microbeads released p53 polyplex specifically in colon and could serve as an effective alternative for CRC therapy.

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“…14 We examined the effects of magnetofection using quaternary polyplexes on mouse bone marrow-derived DC transfection and subsequent maturation (assessed by expression of MHCI, MHCII and CD86) after 5 h incubation with 2 h application of an external magnetic field. As shown in Supplementary Figure S6, the typical purity of CD11c þ DCs, which is the transmembrane protein found at high levels on most DCs, was B70-80%.…”

Section: Resultsmentioning

confidence: 99%

Design of magnetic polyplexes taken up efficiently by dendritic cell for enhanced DNA vaccine delivery

et al. 2013

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Dendritic cells (DC) targeting vaccines require high efficiency for uptake, followed by DC activation and maturation. We used magnetic vectors comprising polyethylenimine (PEI)-coated superparamagnetic iron oxide nanoparticles, with hyaluronic acid (HA) of different molecular weights (<10 and 900 kDa) to reduce cytotoxicity and to facilitate endocytosis of particles into DCs via specific surface receptors. DNA encoding Plasmodium yoelii merozoite surface protein 1-19 and a plasmid encoding yellow fluorescent gene were added to the magnetic complexes with various % charge ratios of HA: PEI. The presence of magnetic fields significantly enhanced DC transfection and maturation. Vectors containing a high-molecular-weight HA with 100% charge ratio of HA: PEI yielded a better transfection efficiency than others. This phenomenon was attributed to their longer molecular chains and higher mucoadhesive properties aiding DNA condensation and stability. Insights gained should improve the design of more effective DNA vaccine delivery systems.

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On designing stable magnetic vectors as carriers for malaria DNA vaccine

Cited by 25 publications

References 47 publications

Magnetic micelles for DNA delivery to rat brains after mild traumatic brain injury

Magnetic micelles for DNA delivery to rat brains after mild traumatic brain injury

Microbeads mediated oral plasmid DNA delivery using polymethacrylate vectors: an effectual groundwork for colorectal cancer

Design of magnetic polyplexes taken up efficiently by dendritic cell for enhanced DNA vaccine delivery

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