The frame set conjecture for B-splines B n , n ≥ 2, states that the frame set is the maximal set that avoids the known obstructions. We show that any hyperbola of the form ab = r, where r is a rational number smaller than one and a and b denote the sampling and modulation rates, respectively, has infinitely many pieces, located around b = 2, 3, . . . , not belonging to the frame set of the nth order B-spline. This, in turn, disproves the frame set conjecture for B-splines. On the other hand, we uncover a new region belonging to the frame set for B-splines B n , n ≥ 2.2010 Mathematics Subject Classification. Primary 42C15. Secondary: 42A60