On-line calorimetry as a technique for process monitoring and control in biotechnology

Stockar, Urs von; Duboc, Philippe; Menoud, L.; Marison, I. W.

doi:10.1016/s0040-6031(97)00055-5

Cited by 44 publications

(22 citation statements)

References 19 publications

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“…A linear correlation was found between heat generation rate and oxygen uptake, cell growth, substrate uptake rates for bioprocesses under strict aerobic conditions. 13 Heat yield coefficients estimated for cell growth, substrate and oxygen uptake rates were proved to be constant for all aerobic biological reactions irrespective of the type of bioprocess. 14 This shows the feasibility of employing metabolic heat as a potential parameter to formulate kinetic equations for effective design and scale-up of bioreactors.…”

Section: Introductionmentioning

confidence: 91%

Bioenergetic studies on aerobic growth of Pseudomonas aeruginosa in a single‐substrate media

Surianarayanan¹,

Sivaprakasam²

2009

J of Chemical Tech & Biotech

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BACKGROUND: The large-scale development of various bioprocesses has posed a problem because of the inherent complexities of biological systems. This is because of the lack of discretion in the use of conventional chemical reactors to carry out complex bioreactions. Existing scale-up methods for bioprocesses are formulated employing offline parameters, i.e. growth rate, substrate uptake rate, oxygen transfer rate and stirring rate per unit volume. Metabolic heat generation is an online process variable portraying the instantaneous activity of any living organism and different types of calorimeters are employed to measure the exothermic heat in non-invasive way.

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Section: Introductionmentioning

confidence: 91%

Bioenergetic studies on aerobic growth of Pseudomonas aeruginosa in a single‐substrate media

Surianarayanan¹,

Sivaprakasam²

2009

J of Chemical Tech & Biotech

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“…Capacitance spectroscopy over a wide range of electric field can be also used to determine the average cell size and the number of cells [24]. Finally, heat generated during growth have been also presented in the past to measure the amount of active cells in the bioreactor [25].…”

Section: Monitoring Physicochemical Parametersmentioning

confidence: 99%

A Self-Contained System With CNTs-Based Biosensors for Cell Culture Monitoring

Boero

Olivo

Carrara

et al. 2012

IEEE J. Emerg. Sel. Topics Circuits Syst.

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Abstract-Biosensors have been applied to disparate fields, especially for endogenous compounds such as glucose and lactate. The main areas of application are certainly related to medical and diagnostic purposes. However, metabolic monitoring can be also of interest in cell analysis. Cells can be cultivated for several purposes, such as understanding and modeling some biological mechanisms, the development of new drugs and therapies, or in the field of regenerative medicine. All the aforementioned applications require a thorough knowledge of the biological system under study. In this paper, we propose the development of a self-contained system based on electrochemical biosensors for cell culture monitoring. The detection is based on oxidases immobilized onto carbon nanotubes. We also develop an architecture to record the signal generated by the biosensor and transmit it to a remote station by means of a Bluetooth module. We calibrate the system for glucose and lactate detection in phosphate buffer solution. We achieve a sensitivity of 55.5 and a detection limit of 2 for glucose, as well as a sensitivity of 25.0 and a detection limit of 11 for lactate. We finally validate the two biosensors for metabolic monitoring in culture medium and we detect lactate production in neuroblastoma cells after 72 h of cultivation. The integrated system proposed in the present work opens new opportunities towards the development of novel tools for cell analysis.

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“…The heat generation and its regulation accompanied by metabolic turnover is an inherent property of living systems [13,14]. …”

Section: Introductionmentioning

confidence: 99%

Cooling System Economy in Ethanol Production Using Thermotolerant Yeast Kluyveromyces Sp. IIPE453

Kumar¹,

Dheeran²,

Singh³

et al. 2013

AJMR

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The growth of thermotolerant/ thermophilic ethanol producing yeast and the fermentation processes using sugary substrates are exothermic processes. If the fermenters are heat insulated, the requirement of heat for maintaining the fermentation broth for ethanol production may be reduced considerably. The heat generated due to growth of thermotolerant yeast Kluyveromyces sp. IIPE453 was found to be 652kJ mol -1 at 50°C using glucose as a substrate. The heat generated due to ethanol formation by Kluyveromyces sp. IIPE453 was found to be 132.54kJ mol -1 of sugar consumed or 67.84kJ mol -1 of ethanol produced at 50°C using sugarcane molasses as substrate. This heat would be sufficient for maintaining the desired temperature, if insulated fermentation systems are used. Therefore, no additional heat would be required to maintain the temperature in fermentation process by thermotolerant yeast at 50°C .

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On-line calorimetry as a technique for process monitoring and control in biotechnology

Cited by 44 publications

References 19 publications

Bioenergetic studies on aerobic growth of Pseudomonas aeruginosa in a single‐substrate media

Bioenergetic studies on aerobic growth of Pseudomonas aeruginosa in a single‐substrate media

A Self-Contained System With CNTs-Based Biosensors for Cell Culture Monitoring

Cooling System Economy in Ethanol Production Using Thermotolerant Yeast Kluyveromyces Sp. IIPE453

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