On‐line capillary electrophoresis‐electrospray ionization mass spectrometry of nucleotides

Zhao, Zhiwei; Wahl, Jon H.; Udseth, Harold R.; Hofstadler, Steven A.; Fuciarelli, Alfred F.; Smith, Richard D.

doi:10.1002/elps.1150160165

Cited by 37 publications

(23 citation statements)

References 26 publications

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“…-modified guanosine monophosphate and adenosine monophosphate [72], anti-7,8,9,10-tetrahydrobenzo[a]pyrene-7,8-diol-9,10-epoxidemodified nucleotides [79], phenyl glycidyl ether-modified nucleotides, and phenyl glycidyl ether 5' phosphate-modified nucleotides [78], NAD 1 and FAD 1 dinucleotides [74], modified nucleosides including benzo[g]chrysene and 5,6-dimethylchrysene-modified deoxypurines [84], and styrene oxide-modified adenosine adducts [85]. By using sample stacking technique, CE-MS reached the detection limit of 6.3610…”

Section: Dna Adducts Damaged Dna and 8-hydroxydeoxyguanosinementioning

confidence: 99%

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Liu

et al. 2004

Electrophoresis

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Investigation of effective biomarkers for cancers is currently a popular area of study in clinical and cancer researches, because it can potentially lead to pre-cancer screening or pre-cancer diagnosis and may provide useful information on cancer type and the disease's stage of progression. More and more biochemical or chemical fluid components of the human body such as urine, blood, and cerebrospinal fluid have been considered to contain biomarkers, which are useful in cancer researches, pre-cancer diagnosis, and cancer follow-ups during or after cancer treatment. Several modern analytical techniques, such as gas chromatography (GC), high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and other separation techniques as well as hyphenated techniques, have been extensively used in study of cancer biomarkers. Among these techniques, CE is considered to be a highly efficient and practical analytical technique because of the small sample volume requirement and its wide separation versatility, ranging from small inorganic molecules to large biomolecules. This review discusses the latest developments involving biomarkers and their analysis by CE, including a discussion of instrumental conditions, method developments, and data analysis.

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Section: Dna Adducts Damaged Dna and 8-hydroxydeoxyguanosinementioning

confidence: 99%

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Liu

et al. 2004

Electrophoresis

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“…Nucleotides [55] and oligonucleotides [52, 55, Fig. 8 as the TIE for the CE/ES/MS analysis of an extract from the PheNodendron wilsonii bark.…”

Section: Biologicalmentioning

confidence: 99%

Recent advances in capillary electrophoresis/electrospray/mass spectrometry

Banks¹

1997

Electrophoresis

284

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Successful on-line interfacing of capillary electrophoresis (CE) with electrospray (ES) mass spectrometry (MS) has progressed substantially in recent years. Of particular note also is the development which has occurred in combining the more advanced capillary-based electromigration separation techniques, such as capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CIT), micellar electrokinetic chromatography (MEKC) and capillary electrochromatography (CEC), with ES/MS. The union of these electromigration schemes with MS detection provides a useful and sensitive analytical tool for the separation, quantitation and identification of biological, therapeutic, environmental and other important classes of chemical analytes. By making optimal use of the characteristics inherent with these separation mechanisms, greatly enhanced MS performance may be obtained. The following review summarizes the significant issues and challenges involved with CE/ES/MS analysis as well as results which have recently been obtained.

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“…In the case of nucleotides, a majority of papers only describe the development of a CE-MS procedure using standard solutions in water [29,[32][33][34]. Of course, in terms of sample cleanup, the many different procedures, developed for CE using UV detection in the field of nucleotide analysis, are equally suitable for CE-MS.…”

Section: Nucleotidesmentioning

confidence: 98%

“…Also enzyme assay, bioluminescence, 31 P nuclear magnetic resonance spectroscopy, and GC can be applied to the determination of nucleotides [20,26]. The high sensitivity and selectivity of MS and tandem MS (MS/MS) techniques provide great separation and detection power, making LC-MS and LC-MS/MS [12,27,28] as well as CE-MS and CE-MS/MS [11,[17][18][19][29][30][31][32][33][34] attractive alternatives for the analysis of nucleotides.…”

Section: Nucleotidesmentioning

confidence: 99%

Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry

et al. 2005

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This review is focused on the capillary electrophoresis-mass spectrometric (CE-MS) analysis of nucleic acid constituents in the broadest sense, going from nucleotides and adducted nucleotides over nucleoside analogues to oligonucleotides. These nucleic acid constituents play an important role in a variety of biochemical processes. Hence, their isolation, identification, and quantification will undoubtedly help reveal the process of life and disease mechanisms, such as carcinogenesis, and can also be useful for antitumor and antiviral drug research to provide valuable information about mechanism of action, pharmacokinetics, pharmacodynamics, toxicity, therapeutic drug level monitoring, and quality control related to this substance class. Fundamental investigations into their structure, the search for modifications, the occurrence and biochemical impact of structural variation amongst others, are therefore of great value. In view of the related bioanalytical procedures, the coupling of CE to MS has emerged as a powerful tool for the analysis of the complex mixtures of nucleic acid constituents: CE confers rapid analysis and efficient resolution, while MS provides high selectivity and sensitivity with structural characterization of minute amounts of compound. After an introduction about the biochemical and analytical perspectives on the nucleic acid constituents, the different modes of CE used in this field of research as well as the relevant CE-MS interfaces and the difficulties associated with quantitative CE-MS are briefly discussed. A large section is finally devoted to field-oriented applications.

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On‐line capillary electrophoresis‐electrospray ionization mass spectrometry of nucleotides

Cited by 37 publications

References 26 publications

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Recent advances in capillary electrophoresis/electrospray/mass spectrometry

Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry

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