On-line estimation of viable cells in a hybridoma culture at various DO levels using ATP balancing and redox potential measurement

Eyer, Kurt; Heinzle, Elmar

doi:10.1002/(sici)1097-0290(19960205)49:3<277::aid-bit5>3.0.co;2-h

Cited by 30 publications

(11 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the culture illustrated in Figure 4, CRP decreased from -50 to -81 mV during the exponential growth phase and then increased during the death phase to a maximum of +34 m V. Similar characteristic variations in CRP and its correlation with viable cell concentration have been reported previously for hybridoma cultures at constant pH and DO (Hwang and Sinskey, 1991;Eyer and Heinzle, 1996;Higareda et aI., 1997). Initiation of the exponential growth phase could be inferred from a decrease in the CRP.…”

Section: On-line Monitoring and Control Of Hhc Culturessupporting

confidence: 85%

Cell Culture Engineering VI

1998

Current Applications of Cell Culture Engineering

View full text Add to dashboard Cite

Section: On-line Monitoring and Control Of Hhc Culturessupporting

confidence: 85%

Cell Culture Engineering VI

1998

Current Applications of Cell Culture Engineering

View full text Add to dashboard Cite

“…Use of porous microcarriers as a cellular microenvironment for cell expansion and prominent protein production may cause oxygen diffusion resistance inside the beads. This influences adenosine triphosphate production and specific substrate uptake rates . Previous work showed that 3D constructs strongly influence cell growth and productivity; some expected that changing to 3D constructs would also influence oxygen consumption .…”

Section: Introductionmentioning

confidence: 99%

Growth, metabolic activity, and productivity of immobilized and freely suspended CHO cells in perfusion culture

Hilal‐Alnaqbi

Zhang

et al. 2013

Biotech and App Biochem

View full text Add to dashboard Cite

Chinese hamster ovary (CHO) cells producing β-galactosidase (β-gal) were successfully cultured on silicone-based porous microcarriers (ImmobaSil FS) in a 1 L stirred-tank perfusion bioreactor. We studied the growth, metabolism, and productivity of free and immobilized cells to understand cellular activity in immobilized conditions. CHO cells attached to ImmobaSil FS significantly better than to other microcarriers. Scanning electron microscope images showed that the CHO cells thoroughly colonized the porous surfaces of the ImmobaSil FS, exhibiting a spherical morphology with microvilli that extended to anchorage cells on the silicone surface. In perfusion culture, the concentration of the attached cells reached 8 × 10(8) cells/mL of carrier, whereas those that remained freely suspended reached 2 × 10(7) cells/mL medium. The β-gal concentration reached more than 5 unit/mL in perfusion culture, more than fivefold that of batch culture. The maximum concentration per microcarrier was proportional to the initial cell density. The specific growth rate, the specific β-gal production rate, the percentage of S phase, and the oxygen uptake rate were all relatively lower for immobilized cells than freely suspended cells in the same bioreactor, indicating that not only do cells survive and grow to a greater extent in a free suspension state, but they are also metabolically more active than viable cells inside the pores of the microcarriers.

show abstract

“…Indirect methods employed conventional sensors to estimate the cell density from oxygen uptake rate (OUR), carbon dioxide evolution rate (CER) or ATP production rate (APR) (Kamen et al 1996;Eyer and Heinzle 1996;Zeiser et al 2000;Konstantinov et al 1994). Nevertheless, they were based on the hypothesis of a constant correlation between cell density and OUR, CER or APR in the culture.…”

Section: Introductionmentioning

confidence: 99%

Real-time monitoring of adherent Vero cell density and apoptosis in bioreactor processes

Petiot¹,

El-Wajgali²,

Esteban³

et al. 2012

Cytotechnology

View full text Add to dashboard Cite

This study proposes an easy to use in situ device, based on multi-frequency permittivity measurements, to monitor the growth and death of attached Vero cells cultivated on microporous microcarriers, without any cell sampling. Vero cell densities were on-line quantified up to 10(6) cell mL(-1). Some parameters which could potentially impact Vero cell morphological and physiological states were assessed through different culture operating conditions, such as media formulation or medium feed-harvest during cell growth phase. A new method of in situ cell death detection with dielectric spectroscopy was also successfully implemented. Thus, through permittivity frequency scanning, major rises of the apoptotic cell population in bioreactor cultures were detected by monitoring the characteristic frequency of the cell population, f(c), which is one of the culture dielectric parameters. Both cell density quantification and cell apoptosis detection are strategic information in cell-based production processes as they are involved in major events of the process, such as scale-up or choice of the viral infection conditions. This new application of dielectric spectroscopy to adherent cell culture processes makes it a very promising tool for risk-mitigation strategy in industrial processes. Therefore, our results contribute to the development of Process Analytical Technology in cell-based industrial processes.

show abstract

On-line estimation of viable cells in a hybridoma culture at various DO levels using ATP balancing and redox potential measurement

Cited by 30 publications

References 15 publications

Cell Culture Engineering VI

Cell Culture Engineering VI

Growth, metabolic activity, and productivity of immobilized and freely suspended CHO cells in perfusion culture

Real-time monitoring of adherent Vero cell density and apoptosis in bioreactor processes

Contact Info

Product

Resources

About