On-line gas dialyzer for automated enzymatic analysis with potentiometric ammonia detection

Fraticelli, Yvonne M.; Meyerhoff, Mark E.

doi:10.1021/ac00253a039

Cited by 36 publications

(9 citation statements)

References 24 publications

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“…The constriction due to the small diameter tubing develops enough positive pressure in the porous PTFE tubing to force the gas bubbles through its wall. Similar applications of microporous PTFE have been previously reported (24,25).…”

Section: Discussionsupporting

confidence: 83%

Automated segmented flow electrochemical analyzer

Jayaweera¹,

Ramaley²

1989

Anal. Chem.

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Section: Discussionsupporting

confidence: 83%

Automated segmented flow electrochemical analyzer

Jayaweera¹,

Ramaley²

1989

Anal. Chem.

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“…as the sniffer. Such tubing had been previously used as an on-line gas dialyzer to remove endogenous ammonia from blood (21), and as a debubbler tube for air-segmented continuous flow analysis (22). While significant response to gas-phase ammonia was achieved with these sniffers, their response times, particularly in going from high to low levels of NH3, were exceedingly long.…”

Section: Resultsmentioning

confidence: 99%

Continuous monitoring of ambient ammonia with a membrane-electrode-based detector

Meyerhoff¹

1987

Anal. Chem.

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Section: Types and Applications Of L-asparaginase-based Biosensorsmentioning

confidence: 99%

“…In 1983, the first ASNase-based biosensor was developed for ammonia detection through the combination of an online gas dialyzer, which successfully removed the high levels of ammonia nitrogen background interference from physiological samples, plus a potentiometric ammonia gas detector [22]. This electrode-based, ammonia-liberating ASNase assay system enabled the quick and accurate concentration analysis of L-asparagine in blood serum samples up to 10 −4 M levels [22]. In 1995, an ASNase-based biosensor was designed for ammonia detection via the combination of ASNase in garlic tissue cells, responsible for L-asparagine transformation into ammonia, subsequently detected by an ammonia gas electrode.…”

Section: Types and Applications Of L-asparaginase-based Biosensorsmentioning

confidence: 99%

L-Asparaginase-Based Biosensors

et al. 2021

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L-asparaginase (ASNase) is an aminohydrolase enzyme widely used in the pharmaceutical and food industries. Although currently its main applications are focused on the treatment of lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) and acrylamide reduction in starch-rich foods cooked at temperatures above 100 °C, its use as a biosensor in the detection and monitoring of L-asparagine levels is of high relevance. ASNase-based biosensors are a promising and innovative technology, mostly based on colorimetric detection since the mechanism of action of ASNase is the catalysis of the L-asparagine hydrolysis, which releases L-aspartic acid and ammonium ions, promoting a medium pH value change followed by color variation. ASNase biosensing systems prove their potential for L-asparagine monitoring in ALL patients, along with L-asparagine concentration analysis in foods, due to their simplicity and fast response.

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On-line gas dialyzer for automated enzymatic analysis with potentiometric ammonia detection

Cited by 36 publications

References 24 publications

Automated segmented flow electrochemical analyzer

Automated segmented flow electrochemical analyzer

Continuous monitoring of ambient ammonia with a membrane-electrode-based detector

L-Asparaginase-Based Biosensors

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