On-Line Hollow-Fiber Flow Field-Flow Fractionation-Electrospray Ionization/Time-of-Flight Mass Spectrometry of Intact Proteins

Reschiglian, Pierluigi; Zattoni, Andrea; Roda, Barbara; Cinque, Leonardo; Parisi, Daniela; Roda, Aldo; Piaz, Fabrizio Dal; Moon, Myeong Hee; Min, Byung Ryul

doi:10.1021/ac048898o

Cited by 71 publications

(64 citation statements)

References 48 publications

(75 reference statements)

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“…Earlier studies of HF FlFFF were focused on the use of HF as a channel for FlFFF [1, 2,11]; the potential of the new technique was enlarged with applications to particles, cells, bacteria, and synthetic organic-soluble polymers [12 -17]. Recently, an attempt has been made to interface HF FlFFF with ESI/ MS online for protein separation and simultaneous characterization [7].…”

Section: Introductionmentioning

confidence: 99%

Performance of hollow‐fiber flow field‐flow fractionation in protein separation

Park

Paeng

Kang

et al. 2005

J of Separation Science

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Since hollow-fiber flow field-flow fractionation (HF FIFFF) utilizes a cylindrical channel made of a hollow-fiber membrane, which is inexpensive and simple in channel assembly and thus disposable, interests are increasing as a potential separation device in cells, proteins, and macromolecules. In this study, performance of HF FIFFF of proteins is described by examining the influence of flow rate conditions and length of fiber (polyacrylonitrile or PAN in this work) on sample recovery as well as experimental plate heights. The interfiber reproducibility in terms of separation time and recovery was also studied. Experiments showed that sample recovery was consistent regardless of the length of fiber when the effective field strength (equivalent to the mean flow velocity at the fiber wall) and the channel void time were adjusted to be equivalent for channels of various fiber lengths. This supported that the majority of sample loss in HF FIFFF separation of apoferritin and their aggregates may occur before the migration process. It is finally demonstrated that HF FIFFF can be applied for characterizing the reduction in Stokes' size of low density lipoproteins from blood plasma samples obtained from patients having coronary artery disease and from healthy donors.

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Section: Introductionmentioning

confidence: 99%

Performance of hollow‐fiber flow field‐flow fractionation in protein separation

Park

Paeng

Kang

et al. 2005

J of Separation Science

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“…FlFFF did not disrupt the noncovalent binding among subunits and between polypeptide chain and prosthetic group. This was clearly proved in the HF FlFFF analysis of apomyoglobin (Mb) with online detection by ESI-TOFMS [44]. …”

Section: Proteins Protein Aggregates and Dnamentioning

confidence: 82%

“…FlFFF systems are available through PostNova Analytics (www.postnova.com), ConSenxus (www.consenxus.com), and Wyatt Technologies (www.wyatt.com). A third configuration that is not commercially available uses a cylindrical tube such as a hollow fiber (HF) membrane [43][44][45] or a ceramic HF [46] (Fig. 2c).…”

Section: Crossflow Fffmentioning

confidence: 99%

Field‐flow fractionation of proteins, polysaccharides, synthetic polymers, and supramolecular assemblies

Williams¹,

Lee

2006

J of Separation Science

125

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This review summarizes developments and applications of flow and thermal field-flow fractionation (FFF) in the areas of macromolecules and supramolecular assemblies. In the past 10 years, the use of these FFF techniques has extended beyond determining diffusion coefficients, hydrodynamic diameters, and molecular weights of standards. Complex samples as diverse as polysaccharides, prion particles, and block copolymers have been characterized and processes such as aggregation, stability, and infectivity have been monitored. The open channel design used in FFF makes it a gentle separation technique for high- and ultrahigh-molecular weight macromolecules, aggregates, and self-assembled complexes. Coupling FFF with other techniques such as multiangle light scattering and MS provides additional invaluable information about conformation, branching, and identity.

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“…It was noted above that FFF optimally requires the field and/or field gradient to be uniform across the breadth of the channel, and that the channel also be of uniform thickness across its breadth. An exception to this arrangement of a thin channel of uniform thickness with a transverse field is the axisymmetrical symmetry of hollow fibre FFF, where a fraction of the fluid flows radially outward through the permeable fibre wall (Jönsson & Carlshaf 1989;Wijnhoven et al 1995;Reschiglian et al 2005). On the other hand, a tubular channel with transverse field is not a suitable design for FFF, as explained by Giddings (2000).…”

Section: Introductionmentioning

confidence: 99%

Characterization of magnetic nanoparticles using programmed quadrupole magnetic field-flow fractionation

Williams

Carpino

Zborowski

2010

Phil. Trans. R. Soc. A.

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Quadrupole magnetic field-flow fractionation is a relatively new technique for the separation and characterization of magnetic nanoparticles. Magnetic nanoparticles are often of composite nature having a magnetic component, which may be a very finely divided material, and a polymeric or other material coating that incorporates this magnetic material and stabilizes the particles in suspension. There may be other components such as antibodies on the surface for specific binding to biological cells, or chemotherapeutic drugs for magnetic drug delivery. Magnetic field-flow fractionation (MgFFF) has the potential for determining the distribution of the magnetic material among the particles in a given sample. MgFFF differs from most other forms of field-flow fractionation in that the magnetic field that brings about particle separation induces magnetic dipole moments in the nanoparticles, and these potentially can interact with one another and perturb the separation. This aspect is examined in the present work. Samples of magnetic nanoparticles were analysed under different experimental conditions to determine the sensitivity of the method to variation of conditions. The results are shown to be consistent and insensitive to conditions, although magnetite content appeared to be somewhat higher than expected.

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On-Line Hollow-Fiber Flow Field-Flow Fractionation-Electrospray Ionization/Time-of-Flight Mass Spectrometry of Intact Proteins

Cited by 71 publications

References 48 publications

Performance of hollow‐fiber flow field‐flow fractionation in protein separation

Performance of hollow‐fiber flow field‐flow fractionation in protein separation

Field‐flow fractionation of proteins, polysaccharides, synthetic polymers, and supramolecular assemblies

Characterization of magnetic nanoparticles using programmed quadrupole magnetic field-flow fractionation

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