On-line identification of secondary metabolites in freshwater microalgae and cyanobacteria by combined liquid chromatography–photodiode array detection-mass spectrometric techniques

Frassanito, Rita; Cantonati, Marco; Tardio, Massimiliano; Mancini, Ines; Guella, Graziano

doi:10.1016/j.chroma.2005.02.066

Cited by 30 publications

(26 citation statements)

References 20 publications

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“…Although it is difficult to tell from the lack of relative percent distribution data for MGDG and DGDG, the G. sanguineum examined by Guella et al (2003) would appear to fall into Cluster 1 because of the presence of predominantly C 18 /C 18 DGDG. The same is true for the alternative form of G. sanguineum examined by Frassanito et al (2005). It is difficult to place the K. veneficum examined by Adolf et al (2007) into either cluster because the data are not directly comparable.…”

Section: Discussionmentioning

confidence: 89%

“…Since the work of Guella et al (2003), ESI/MS and ESI/MS/MS have been used to a limited extent in the examination of a form of G. sanguineum with an alternative, non-peridinin-containing endosymbiont (Frassanito et al, 2005) and Karlodinium veneficum (Adolf et al, 2007). Although it is difficult to tell from the lack of relative percent distribution data for MGDG and DGDG, the G. sanguineum examined by Guella et al (2003) would appear to fall into Cluster 1 because of the presence of predominantly C 18 /C 18 DGDG.…”

Section: Discussionmentioning

confidence: 99%

“…It is only recently that facile exploration has been made into which acylglycerolipids contain which fatty acids (Guella et al, 2003;Frassanito et al, 2005;Adolf et al, 2007). The limitations of GC/MS can be overcome with electrospray ionization/mass spectrometry (ESI/MS), an essential component of lipidomic research (Han & Gross, 2003), and electrospray ionization/mass spectrometry/mass spectrometry (ESI/MS/MS).…”

Section: Introductionmentioning

confidence: 99%

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Mono- and digalactosyldiacylglycerol composition of dinoflagellates. I. Peridinin-containing taxa

Gray¹,

Lasiter²,

Leblond³

2009

European Journal of Phycology

108

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Two glycolipids, monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), are components of chloroplast membranes. This study addresses the lack of information regarding the major intact forms of these glycolipids in photosynthetic dinoflagellates through the use of a full-scan positive-ion electrospray ionization/mass spectrometry (ESI/ MS) survey of MGDG and DGDG in 35 peridinin-containing taxa from the class Dinophyceae. Further analysis using positive-ion electrospray ionization/mass spectrometry/mass spectrometry (ESI/MS/MS) was performed to determine the positional distribution of fatty acids associated with MGDG and DGDG. The dinoflagellates examined were divided into two clusters based on the forms of MGDG and DGDG present. The first possessed 18:5/18:4 MGDG (sn

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mono- and digalactosyldiacylglycerol composition of dinoflagellates. I. Peridinin-containing taxa

Gray¹,

Lasiter²,

Leblond³

2009

European Journal of Phycology

108

View full text Add to dashboard Cite

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“…Chromatographic peaks were identified by comparing retention times and online spectra (UV-Vis and positive and negative-ion ESI-MS spectra) against known standards (FraSSanito et al 2005). …”

Section: Molecular Data Generationmentioning

confidence: 99%

Multifaceted characterization of a Lemanea fluviatilis population (Batrachospermales, Rhodophyta) from a glacial stream in the south-eastern Alps

Saber¹,

Cantonati²,

Vis³

et al. 2016

Fottea

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Abstract:The aim of this study was a combined and multifaceted characterization (morphological, molecular, lipid, pigment, and ecological data) of a Lemanea (freshwater red alga) population from the south-eastern Alps, exploring its adaptive strategies to the montane habitat, (turbulent, very-cold glacial stream with extremely low-conductivity). Although the thalli were small (only up to 1 cm), the morphology was within the current circumscription of Lemanea fluviatilis. The molecular data placed this population within a clade of specimens identified as L. fluviatilis and L. fucina. This L. fluviatilis population was determined to possess lipid classes, especially phosphatidylcholine and monogalactosyldiacylglycerol with high unsaturation index (UI) and long acyl chains, which are typical adaptations for maintaining adequate membrane fluidity and consequently all the metabolic processes associated to the plasma membrane. The carotenoids profile revealed that, besides α /β-carotene, there are significant amounts of zeaxanthin and lutein. This study further demonstrated that red algae are a rich source of important food web w-3 fatty acids and may play an important role in the diets of grazers. L. fluviatilis is reported from one of the highest elevations (2,170 m a.s.l.) known for the genus Lemanea and this species. This study confirms the presence of L. fluviatilis in a cold, unpolluted, turbulent stream and this type of stream may be its preferred habitat.

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“…The extractions were combined, centrifuged (10 min, 2000 g), reduced to dryness by rotary evaporation, redissolved in a measured volume of acetone/methanol (1:1) and stored in amber glass vials under nitrogen until the time of analysis. HPLC/PDAD-MS was performed on the raw extracts according to Frassanito et al (2005) on a Hewlett-Packard Model 1100 Series liquid chromatograph coupled both to a PDA (Photo Diode-Array) detector (Agilent 1100 Series) and to a Bruker Esquire-LC quadrupole ion trap mass spectrometer equipped with atmospheric pressure ESI interface (negative-ion mode). Fucoxanthin can be used as a precise marker for epilithic diatoms, because other algal groups containing this accessory pigment (in particular chrysophytes) are virtually absent from the phytobenthos in freshwater lakes (e.g.…”

Section: Sampling Data Collection and Analysismentioning

confidence: 99%

Environmental controls of epilithic diatom depth-distribution in an oligotrophic lake characterized by marked water-level fluctuations

Cantonati

Scola

Angeli

et al. 2009

European Journal of Phycology

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The depth-distribution of epilithic diatoms in a carbonate meromictic lake (south-eastern Alps), characterized by marked water-level fluctuations, was investigated. Fixed stations were placed along a depth-profile at intervals of 2-3 m and sampled throughout the year using scuba diving. Diatom analysis included quantification of living cells (biovolume calculations) and the estimation of fucoxanthin concentration, which, coupled with the analysis of digested material, permitted taxonomic determination at specific or subspecific level. Multivariate analyses indicated the following variables to be significant: water-level fluctuations, photosynthetically active radiation, silica and nitrates. Diatoms had distinct depth-distributions. Quantification of densities, biovolumes and Chl a, and physiological (senescence index, fucoxanthin), functional (photosynthetic efficiency), and community structure (diversity) parameters allowed the definition of three depth-distribution zones (shallow, mid-depth and deep). The different diatom communities in these zones were confirmed by ANOSIM (analysis of similarity). The shallow, mid-depth, and deep zone were characterized by disturbance due to water-level fluctuations, high stability with favourable growth conditions and severe light limitation, respectively. Community composition and diversity depth-distribution features remained relatively stable throughout the year, while marked changes in benthic diatom biovolumes were modulated by competition for light with phytoplankton. Fucoxanthin was strongly correlated with epilithic diatom biovolumes. The results indicate that depth-distribution patterns should be considered when performing lake diatom biodiversity inventories and integrity evaluations because water-level fluctuations are likely to increase in many lakes due to increased withdrawal of water and climate change in the future.

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On-line identification of secondary metabolites in freshwater microalgae and cyanobacteria by combined liquid chromatography–photodiode array detection-mass spectrometric techniques

Cited by 30 publications

References 20 publications

Mono- and digalactosyldiacylglycerol composition of dinoflagellates. I. Peridinin-containing taxa

Mono- and digalactosyldiacylglycerol composition of dinoflagellates. I. Peridinin-containing taxa

Multifaceted characterization of a Lemanea fluviatilis population (Batrachospermales, Rhodophyta) from a glacial stream in the south-eastern Alps

Environmental controls of epilithic diatom depth-distribution in an oligotrophic lake characterized by marked water-level fluctuations

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