On-line measurement of extracellular enzymes during fermentation by using membrane techniques

Kroner, K. H.; Kula, Maria‐Regina

doi:10.1016/s0003-2670(00)81488-9

Cited by 53 publications

(3 citation statements)

References 14 publications

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“…The mobile phase was a 0.025 M phosphate buffer at pH 7.5, pumped with a flow rate of 0.6 mL/min. Data acquisition and processing were performed by a chromatography workstation (Baseline 810, Millipore; 12 in Figure 1). The signal from the detector was also monitored on a chart recorder [Model 2210, LKB (not shown in Figure 1)].…”

Section: Methodsmentioning

confidence: 99%

On-line fermentation process monitoring of carbohydrates and ethanol using tangential-flow filtration and column liquid chromatography

Buttler¹,

Johansson²,

Gorton³

et al. 1993

Anal. Chem.

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This paper describes a fully automated on-line system for the unattended monitoring of bioprocesses.Removal of solids and macromolecules from fermentation broth is achieved with a tangential flow filtration unit (Waters Filter/Acquisition Module). The obtained filtrate is transported in a flow system to a cleanup step, based on solid-phase extraction, followed by introduction into a column liquid chromatographic system.Carbohydrates are then detected by refractive index and ethanol content using a laboratory-made selective amperometric alcohol biosensor. Data acquisition and processing, as well as switching of the system valves, are performed by a computer. The on-line system was used for continuous monitoring of five carbohydrates and ethanol during 24-h ethanol fermentations. Waste water from the Swedish pulp industry was used as the technical substrate. Typical RSD values of the entire setup were on the order of 5-6% over a 24-h period of operation.

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Section: Methodsmentioning

confidence: 99%

On-line fermentation process monitoring of carbohydrates and ethanol using tangential-flow filtration and column liquid chromatography

Buttler¹,

Johansson²,

Gorton³

et al. 1993

Anal. Chem.

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“…It is therefore import ant to minimize both the residence time in the loop and the ratio of loop volume to working volume of the bioreactor. At Center for Process Biotechnology two external membrane modules have been tested for sampling from P. chrysogenum cultivations: 1) the Biopem (from B. Braun-Melsungen) [Kroner and Kula, 1984;Kroner and Papamichael, 1988] and 2) a spiral shaped cross flow module [Gam et al, 1989]. In the Biopem the medium is pumped through a chamber (volume 175 ml) containing a magnetically stirred rod, which ensures a tangential flow across the membrane which is placed in the bottom of the chamber [Kroner and Papamichael, 1988].…”

Section: On-line Flow Injection Analysismentioning

confidence: 99%

Physiological Engineering

1997

Physiological Engineering Aspects of Penicillium Chrysogenum

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“…Therefore, on-line sampling devices that can reliably take a representative cell-free sample are needed [1][2][3][4][5][6]. pharmaceutical, chemical and food industries), as well as in research, a broad variety of cell cultivation processes take place in bioreactors.…”

Section: Introductionmentioning

confidence: 99%

A new version of an in situ sampling system for bioprocess analysis

Hilmer

Scheper

1996

Acta Biotechnologica

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In this article, attention is focussed on an on-line sampling device for bioreactors and its characterization regarding sterility and response time. The integration of this device into analytical systems for biotechnology (FIA with biosensors) resulted in on-line analysis systems for bioprocess control. The new E S P version of an in situ sampling system for bioprocess analysis produced by the EPPENDORF-NETHELER-HINZ GmbH. Germany, had a short response time of 8 min (99%), which was determined by means of conductivity measurement after an increase of the medium conductivity due to the gradual addition of KCI. Effectiveness and reliability of the module were tested by bubble point measurement resulting in a.bubble point pressure of 2.1 bars. The sampling probe was tested successfully for use in a broad.variety of microorganisms and cultivations.

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On-line measurement of extracellular enzymes during fermentation by using membrane techniques

Cited by 53 publications

References 14 publications

On-line fermentation process monitoring of carbohydrates and ethanol using tangential-flow filtration and column liquid chromatography

On-line fermentation process monitoring of carbohydrates and ethanol using tangential-flow filtration and column liquid chromatography

Physiological Engineering

A new version of an in situ sampling system for bioprocess analysis

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