Demand for cleaner energy sources both as additive and alternative fuels that can substitute or contribute to the usage of conventional fuels is growing. Researchers have mainly focused on improving or finding a renewable fuel from vegetable oil or addition of chemicals and alcohols for IC engine. This study analyzed the effects of a high percentage of dimethyl ether (DME) combined with biodiesel in a diesel engine. Transesterification method was selected for conversion of pure safflower oil to biodiesel. DME was blended with biodiesel at concentrations of 50% and 25% on a volume basis, respectively. Engine performance and emissions tests demonstrated that the thermal efficiency values were increased at high load operation when the engine was fueled with high percentage of DME. Furthermore, compared to conventional diesel, there has been a notable decrease in NOx emissions. Nevertheless, the introduction of DME blend had negligible effects on CO2 emissions. However, when using a high ratio of DME blend, HC emissions were found to increase, whereas a low ratio of DME blend resulted in decreased HC emissions. Apart from these, some irregularities were observed both on heat release rate and cylinder pressure especially for 50% of DME usage. Finally, the values for both brake-specific fuel consumption and mass fuel of DME-blended fuels were deteriorated.