On-line monitoring of airborne bioaerosols released from a composting/green waste site

O’Connor, David J.; Daly, Shane; Sodeau, John R.

doi:10.1016/j.wasman.2015.04.015

Cited by 53 publications

(32 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Over the entire "light" working day, the average fluorescent proportion of total particles counted was~1% (FAP = 109.9 particles L −1 ). On the "heavy" working day, the average fluorescent proportion of total particles counted was~7% (FAP = 304.2 Particles L −1 ) and at the weekend, the average fluorescent proportion of total particles counted was~18% (FAP = 320.2 Particles L −1 ) [129]. It was noted that far fewer particles were collected at the weekend (particles sizes between 0.5-13 µm).…”

Section: Occupational Site Campaignsmentioning

confidence: 98%

“…The overall campaign was carried out for a seven-day period between 30th of September and 17th of October 2014 [129]. Three short term visits were undertaken, two during weekdays and one at the weekend when the site was formally closed.…”

Section: Occupational Site Campaignsmentioning

confidence: 99%

“…The weekday campaigns were categorised by the site manager in terms of workload: one as a "light" workload period and the second as a "heavy" workload period. Each campaign measured four different FAP profiles: (a) Size (µm) vs. Time (h) fluorescent number-concentration plots; (b) AF value ("shape") vs. time (h) fluorescent number-concentration plots; (c) Wind rose graphics for fluorescent number-concentrations with wind speed and direction; and, (d) Effect of relative humidity on fluorescent number counts [129]. The data collected showed that the FAP measurements for all three campaigns accumulated in one principle size regime (~0.5 µm to~3 µm).…”

Section: Occupational Site Campaignsmentioning

confidence: 99%

See 2 more Smart Citations

Review: The Use of Real-Time Fluorescence Instrumentation to Monitor Ambient Primary Biological Aerosol Particles (PBAP)

Fennelly

Sewell²,

Prentice

et al. 2017

Atmosphere

View full text Add to dashboard Cite

Primary biological aerosol particles (PBAP) encompass many particle types that are derived from several biological kingdoms. These aerosol particles can be composed of both whole living units such as pollen, bacteria, and fungi, as well as from mechanically formed particles, such as plant debris. They constitute a significant proportion of the overall atmospheric particle load and have been linked with adverse health issues and climatic effects on the environment. Traditional methods for their analysis have focused on the direct capture of PBAP before subsequent laboratory analysis. These analysis types have generally relied on direct optical microscopy or incubation on agar plates, followed by time-consuming microbiological investigation. In an effort to address some of these deficits, real-time fluorescence monitors have come to prominence in the analysis of PBAP. These instruments offer significant advantages over traditional methods, including the measurement of concentrations, as well as the potential to simultaneously identify individual analyte particles in real-time. Due to the automated nature of these measurements, large data sets can be collected and analyzed with relative ease. This review seeks to highlight and discuss the extensive literature pertaining to the most commonly used commercially available real-time fluorescence monitors (WIBS, UV-APS and BioScout). It discusses the instruments operating principles, their limitations and advantages, and the various environments in which they have been deployed. The review provides a detailed examination of the ambient fluorescent aerosol particle concentration profiles that are obtained by these studies, along with the various strategies adopted by researchers to analyze the substantial data sets the instruments generate. Finally, a brief reflection is presented on the role that future instrumentation may provide in revolutionizing this area of atmospheric research.

show abstract

Section: Occupational Site Campaignsmentioning

confidence: 98%

Section: Occupational Site Campaignsmentioning

confidence: 99%

Section: Occupational Site Campaignsmentioning

confidence: 99%

See 1 more Smart Citation

Review: The Use of Real-Time Fluorescence Instrumentation to Monitor Ambient Primary Biological Aerosol Particles (PBAP)

Fennelly

Sewell²,

Prentice

et al. 2017

Atmosphere

View full text Add to dashboard Cite

show abstract

“…Both the WIBS and UV-APS, in various version updates, have been applied to many types of studies regarding outdoor aerosol characterization. For example, they have been important instruments: in the study of ice nuclei Mason et al, 2015;Twohy et al, 2016), toward the understanding of outdoor fungal spore concentrations (Gosselin et al, 2016;Saari et al, 2015a;O'Connor et al, 2015b), to investigate the concentration and properties of bioaerosols from long-range transport (Hallar et al, 2011), in tropical aerosol Whitehead et al, 2010Whitehead et al, , 2016Huffman et al, 2012;Valsan et al, 2016), in urban aerosol Saari et al, 2015b;Yu et al, 2016), from composting centers (O'Connor et al, 2015b), at high altitude Gabey et al, 2013;Perring et al, 2015;Ziemba et al, 2016), and in many other environments (Healy et al, 2014;Li et al, 2016;O'Connor et al, 2015a). The same instrumentation has been utilized for a number of studies involving the built, or indoor, environment as well (Wu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Systematic characterization and fluorescence threshold strategies for the wideband integrated bioaerosol sensor (WIBS) using size-resolved biological and interfering particles

et al. 2017

View full text Add to dashboard Cite

Abstract. Atmospheric particles of biological origin, also referred to as bioaerosols or primary biological aerosol particles (PBAP), are important to various human health and environmental systems. There has been a recent steep increase in the frequency of published studies utilizing commercial instrumentation based on ultraviolet laser/light-induced fluorescence (UV-LIF), such as the WIBS (wideband integrated bioaerosol sensor) or UV-APS (ultraviolet aerodynamic particle sizer), for bioaerosol detection both outdoors and in the built environment. Significant work over several decades supported the development of the general technologies, but efforts to systematically characterize the operation of new commercial sensors have remained lacking. Specifically, there have been gaps in the understanding of how different classes of biological and non-biological particles can influence the detection ability of LIF instrumentation. Here we present a systematic characterization of the WIBS-4A instrument using 69 types of aerosol materials, including a representative list of pollen, fungal spores, and bacteria as well as the most important groups of non-biological materials reported to exhibit interfering fluorescent properties. Broad separation can be seen between the biological and non-biological particles directly using the five WIBS output parameters and by taking advantage of the particle classification analysis introduced by Perring et al. (2015). We highlight the importance that particle size plays on observed fluorescence properties and thus in the Perring-style particle classification. We also discuss several particle analysis strategies, including the commonly used fluorescence threshold defined as the mean instrument background (forced trigger; FT) plus 3 standard deviations (σ ) of the measurement. Changing the particle fluorescence threshold was shown to have a significant impact on fluorescence fraction and particle type classification. We conclude that raising the fluorescence threshold from FT + 3σ to FT + 9σ does little to reduce the relative fraction of biological material considered fluorescent but can significantly reduce the interference from mineral dust and other non-biological aerosols. We discuss examples of highly fluorescent interfering particles, such as brown carbon, diesel soot, and cotton fibers, and how these may impact WIBS analysis and data interpretation in various indoor and outdoor environments. The performance of the particle asymmetry factor (AF) reported by the instrument was assessed across particle types as a function of particle size, and comments on the reliability of this parameter are given. A comprehensive online supplement is provided, which includes size distributions broken down by fluorescent particle type for all 69 aerosol materials and comparing threshold strategies. Lastly, the study was designed to propose analysis strategies that may be useful to the broader community of UV-LIF instrumentation users in order to promote deeper discussions about how best to continue i...

show abstract

“…Occupational activities involving high levels of biological material such as wastewater treatment plants, biowaste facilities, animal farms and agriculture are of a great concern especially for workers. Recent studies have shown that respiratory diseases such allergies, asthma or chronic obstructive pulmonary disease among others can be linked to bioaerosols exposure [2].…”

Section: Introductionmentioning

confidence: 99%

Sampling Microbial Volatile Organic Compounds: Optimisation of Flow Rate and Sampling Time

Alcega

Tyrrel

Coulon

2017

WIT Transactions on Ecology and the Environment

View full text Add to dashboard Cite

The impact of bioaerosols emissions from urban, agricultural and industrial environments on local air quality is of growing policy concern. However, there is no standardised protocol established yet, despite a large number of bioaerosols sampling methods in use. Additionally, capturing sufficient amounts of material to allow reproducible separation and detection of molecular patterns is still difficult. Chemical fingerprint analysis of microbial volatile organic compounds (MVOC) is a potentially rapid and reproducible approach for the early detection and identification of outdoor contamination as it has been shown to be a successful approach for indoor environments and it can be done on a fine-scale, allowing the identification of species-specific volatiles that may serve as marker compounds for the selective detection of pathogens. In this study we have tested the number and concentration of MVOCs collected using different sampling conditions: 10 min sampling time with variable flow rate (100, 500 and 1000 ml min -1 ) and 100 ml min -1 flow rate during 10, 20 and 30 min using Tenax®-Carbotrap thermal desorption (TD) tubes attached to portable GilAir® air pumps. Our aim was to determine the best sampling conditions in order to get enough material allowing reproducible data of the microbial markers present in outdoor environments. Substantial loses (>50%) of MVOCs occurred when sampling at flow rates higher than 100 ml min -1. 10 min sampling time allowed the collection of most of the MVOCs present in the air (~96%). The optimal sampling settings that allowed the collection of higher concentrations of MVOCs without breakthrough was 10 min sampling at 100 ml min ).

show abstract

On-line monitoring of airborne bioaerosols released from a composting/green waste site

Cited by 53 publications

References 60 publications

Review: The Use of Real-Time Fluorescence Instrumentation to Monitor Ambient Primary Biological Aerosol Particles (PBAP)

Review: The Use of Real-Time Fluorescence Instrumentation to Monitor Ambient Primary Biological Aerosol Particles (PBAP)

Systematic characterization and fluorescence threshold strategies for the wideband integrated bioaerosol sensor (WIBS) using size-resolved biological and interfering particles

Sampling Microbial Volatile Organic Compounds: Optimisation of Flow Rate and Sampling Time

Contact Info

Product

Resources

About